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International Journal of Molecular Medicine
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Article

WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines

  • Authors:
    • Junjie Qu
    • Wen Lu
    • Bilan Li
    • Cong Lu
    • Xiaoping Wan
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The International Peace Maternity and Child Health Hospital of China Welfare Institute affiliated with Shanghai Jiao Tong University, Shanghai 200032, P.R. China, Shanghai First People's Hospital, Shanghai 200080, P.R. China
  • Pages: 1139-1147
    |
    Published online on: March 21, 2013
       https://doi.org/10.3892/ijmm.2013.1314
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Abstract

Cervical cancer is the second most common gynecological malignancy, but the molecular events involved in its development remain unclear. The tumor‑suppressor gene, WW domain-containing oxidoreductase (WWOX), has been found to be lost in various types of cancers. Few studies have been reported detailing the function of WWOX in human cervical cancer; therefore we aimed to investigate the role played by WWOX in human cervical cancer. Immunohistochemistry was used to study preinvasive and invasive primary cervical cancer. Full length cDNA was transfected into HeLa cells to overexpress WWOX, and short hairpin RNA (shRNA) was transfected into SiHa cells to deplete its expression, respectively. The cellular levels of WWOX RNA and protein were detected by real-time PCR and western immunoblotting. Proliferation rates were assessed by methyl thiazolyl tetrazolium (MTT), plate colony formation and soft agar colony assays. Cellular apoptosis was measured by flow cytometry and TdT-mediated dUTP nick-end labeling (TUNEL) assay. The activity of caspase-3 and its protein levels were determined by caspase-3 activity assay and western blot analysis. Xenografts were established by injecting cells into nude mice. The results showed that WWOX expression was decreased in human cervical cancer and cervical cancer cell lines. Reconstitution of WWOX in HeLa cells inhibited their proliferation and induced apoptosis, while knockdown of WWOX in SiHa cells promoted proliferation and inhibited apoptosis. Xenografts in groups of mice verified the effect in vivo. These data suggest that underexpression of WWOX is associated with cervical cancer development. Modulation of WWOX expression may be an effective and novel method for the treatment of cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Qu J, Lu W, Li B, Lu C and Wan X: WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines. Int J Mol Med 31: 1139-1147, 2013.
APA
Qu, J., Lu, W., Li, B., Lu, C., & Wan, X. (2013). WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines. International Journal of Molecular Medicine, 31, 1139-1147. https://doi.org/10.3892/ijmm.2013.1314
MLA
Qu, J., Lu, W., Li, B., Lu, C., Wan, X."WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines". International Journal of Molecular Medicine 31.5 (2013): 1139-1147.
Chicago
Qu, J., Lu, W., Li, B., Lu, C., Wan, X."WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines". International Journal of Molecular Medicine 31, no. 5 (2013): 1139-1147. https://doi.org/10.3892/ijmm.2013.1314
Copy and paste a formatted citation
x
Spandidos Publications style
Qu J, Lu W, Li B, Lu C and Wan X: WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines. Int J Mol Med 31: 1139-1147, 2013.
APA
Qu, J., Lu, W., Li, B., Lu, C., & Wan, X. (2013). WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines. International Journal of Molecular Medicine, 31, 1139-1147. https://doi.org/10.3892/ijmm.2013.1314
MLA
Qu, J., Lu, W., Li, B., Lu, C., Wan, X."WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines". International Journal of Molecular Medicine 31.5 (2013): 1139-1147.
Chicago
Qu, J., Lu, W., Li, B., Lu, C., Wan, X."WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines". International Journal of Molecular Medicine 31, no. 5 (2013): 1139-1147. https://doi.org/10.3892/ijmm.2013.1314
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