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International Journal of Molecular Medicine
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Article

Wasf2: A novel target of intermittent parathyroid hormone administration

  • Authors:
    • Maki Uyama
    • Masamitsu Kawanami
    • Masato Tamura
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan, Department of Periodontology and Endodontology, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan
  • Pages: 1243-1247
    |
    Published online on: March 22, 2013
       https://doi.org/10.3892/ijmm.2013.1315
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Abstract

Systemic intermittent administration of parathyroid hormone (PTH) stimulates bone formation in animals and humans, and recombinant human PTH1-34 (teriparatide) is used clinically for the treatment of osteoporosis. In this study, we investigated the regulation of gene expression by intermittent PTH administration in MC3T3-E1 osteoblastic cells. We found that intermittent PTH1-34 administration downregulated Wiskott-Aldrich syndrome protein family member (Wasf) 2 mRNA expression. Wnt inhibitor, IWP-2, and protein kinase C inhibitor, Go6976, inhibited this downregulation. However, continuous PTH did not affect Wasf2 expression. Transfection of Wasf2 siRNA reduced bone sialoprotein (BSP) mRNA expression in a similar manner following intermittent PTH administration in MC3T3-E1 cells. These results identify Wasf2 as a novel target of intermittent PTH administration via the Wnt and phosphoinositide-dependent protein kinase signaling pathways, and the resulting regulation of BSP expression may contribute to the anabolic effects of PTH.
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Copy and paste a formatted citation
Spandidos Publications style
Uyama M, Kawanami M and Tamura M: Wasf2: A novel target of intermittent parathyroid hormone administration. Int J Mol Med 31: 1243-1247, 2013.
APA
Uyama, M., Kawanami, M., & Tamura, M. (2013). Wasf2: A novel target of intermittent parathyroid hormone administration. International Journal of Molecular Medicine, 31, 1243-1247. https://doi.org/10.3892/ijmm.2013.1315
MLA
Uyama, M., Kawanami, M., Tamura, M."Wasf2: A novel target of intermittent parathyroid hormone administration". International Journal of Molecular Medicine 31.5 (2013): 1243-1247.
Chicago
Uyama, M., Kawanami, M., Tamura, M."Wasf2: A novel target of intermittent parathyroid hormone administration". International Journal of Molecular Medicine 31, no. 5 (2013): 1243-1247. https://doi.org/10.3892/ijmm.2013.1315
Copy and paste a formatted citation
x
Spandidos Publications style
Uyama M, Kawanami M and Tamura M: Wasf2: A novel target of intermittent parathyroid hormone administration. Int J Mol Med 31: 1243-1247, 2013.
APA
Uyama, M., Kawanami, M., & Tamura, M. (2013). Wasf2: A novel target of intermittent parathyroid hormone administration. International Journal of Molecular Medicine, 31, 1243-1247. https://doi.org/10.3892/ijmm.2013.1315
MLA
Uyama, M., Kawanami, M., Tamura, M."Wasf2: A novel target of intermittent parathyroid hormone administration". International Journal of Molecular Medicine 31.5 (2013): 1243-1247.
Chicago
Uyama, M., Kawanami, M., Tamura, M."Wasf2: A novel target of intermittent parathyroid hormone administration". International Journal of Molecular Medicine 31, no. 5 (2013): 1243-1247. https://doi.org/10.3892/ijmm.2013.1315
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