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International Journal of Molecular Medicine
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Article

Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells

  • Authors:
    • Yun-Hee Sung
    • Mal-Soon Shin
    • Il-Gyu Ko
    • Sung-Eun Kim
    • Chang-Ju Kim
    • Hyun-Jong Ahn
    • Hye-Sun Yoon
    • Bong-Jae Lee
  • View Affiliations / Copyright

    Affiliations: Department of Physical Therapy, Kyungnam University, Changwon 631-701, Republic of Korea, Department of Physiology, Kyung Hee University College of Medicine, Seoul 130-701, Republic of Korea, Department of Microbiology, Kyung Hee University College of Medicine, Seoul 130-701, Republic of Korea, Department of Pediatrics, Eulji General Hospital, Eulji University School of Medicine, Seoul 139-872, Republic of Korea, Department of Anesthesiology and Pain Medicine, Kang Dong Kyung Hee Hospital, Kyung Hee University College of Medicine, Seoul 134-890, Republic of Korea
  • Pages: 1030-1036
    |
    Published online on: March 27, 2013
       https://doi.org/10.3892/ijmm.2013.1322
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Abstract

Ulinastatin is an intrinsic serine-protease urinary trypsin inhibitor that can be extracted and purified from human urine. Urinary trypsin inhibitors are widely used to treat patients with acute inflammatory disorders, such as shock and pancreatitis. However, although the anti-inflammatory activities of urinary trypsin inhibitors have been investigated, the mechanisms underlying their actions are not yet fully understood. In the present study, we evaluated the effect of ulinastatin on lipopolysaccharide (LPS)-induced inflammation in relation with nuclear factor-κB (NF-κB) activation using BV2 mouse microglial cells. To accomplish this, we performed a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis, electrophoretic mobility gel shift assay (EMSA), prostaglandin E2 (PGE2) immunoassay and nitric oxide (NO) detection. The results demonstrated that ulinastatin suppressed PGE2 synthesis and NO production by inhibiting the LPS-induced mRNA and protein expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) in BV2 mouse microglial cells. Ulinastatin suppressed the activation of NF-κB in the nucleus. These findings demonstrate that ulinastatin exerts analgesic and anti-inflammatory effects that possibly occur via the suppression of COX-2 and iNOS expression through the downregulation of NF-κB activity.
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Copy and paste a formatted citation
Spandidos Publications style
Sung Y, Shin M, Ko I, Kim S, Kim C, Ahn H, Yoon H and Lee B: Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. Int J Mol Med 31: 1030-1036, 2013.
APA
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H. ... Lee, B. (2013). Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. International Journal of Molecular Medicine, 31, 1030-1036. https://doi.org/10.3892/ijmm.2013.1322
MLA
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H., Yoon, H., Lee, B."Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells". International Journal of Molecular Medicine 31.5 (2013): 1030-1036.
Chicago
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H., Yoon, H., Lee, B."Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells". International Journal of Molecular Medicine 31, no. 5 (2013): 1030-1036. https://doi.org/10.3892/ijmm.2013.1322
Copy and paste a formatted citation
x
Spandidos Publications style
Sung Y, Shin M, Ko I, Kim S, Kim C, Ahn H, Yoon H and Lee B: Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. Int J Mol Med 31: 1030-1036, 2013.
APA
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H. ... Lee, B. (2013). Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. International Journal of Molecular Medicine, 31, 1030-1036. https://doi.org/10.3892/ijmm.2013.1322
MLA
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H., Yoon, H., Lee, B."Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells". International Journal of Molecular Medicine 31.5 (2013): 1030-1036.
Chicago
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H., Yoon, H., Lee, B."Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells". International Journal of Molecular Medicine 31, no. 5 (2013): 1030-1036. https://doi.org/10.3892/ijmm.2013.1322
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