Proteomic analysis of NGF-induced transdifferentiation of adrenal medullary cells

Hu,Cheng-Ping; Wu,Xiao-Rong; Li,Qiu-Gen; Sun,Zuo-Wei; Wang,Ai-Ping; Feng,Jun-Tao; Wang,Jun

doi:10.3892/ijmm.2013.1387

August 2013 Volume 32 Issue 2

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August 2013 Volume 32 Issue 2

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Article

Proteomic analysis of NGF-induced transdifferentiation of adrenal medullary cells

Authors:
- Cheng-Ping Hu
- Xiao-Rong Wu
- Qiu-Gen Li
- Zuo-Wei Sun
- Ai-Ping Wang
- Jun-Tao Feng
- Jun Wang
View Affiliations / Copyright

Affiliations: Department of Respiratory Medicine, Central South University, Xiangya Hospital, Changsha, Hunan, P.R. China, Department of Ophthalmology, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, P.R. China, Second Department of Respiratory Disease, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi, P.R. China, Second Department of Respiratory Disease, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi, P.R. China
Pages: 347-354
|
Published online on: May 21, 2013

https://doi.org/10.3892/ijmm.2013.1387
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Abstract

Nerve growth factor (NGF) is a polypeptide growth factor with specific trophic function in nerve cells and was initially investigated for its role as a key player in the regulation of peripheral innervations. The aim of this study was to examine the NGF-induced transdifferentiation of adrenal medullary cells, and to screen the major candidate differentially expressed proteins involved in the transdifferentiation. NGF was used to treat primary cultures of neonatal calf adrenal medullary cells and the effects of transdifferentiation were determined in association with cellular morphology, ultrastructure and changes in endocrine function. Differentially expressed proteins were screened and identified through two-dimensional gel electrophoresis and mass spectrometry. The protein spots showing differential expression were verified by western blot analysis. We observed neurite outgrowth in the adrenal medullary cells treated with NGF under a phase contrast microscope. Ultrastructure analysis revealed that there were rich drumstick-like and villiform processes on the cell membranes and vesicles were formed near the cell membranes. The cytoplasm was rich in mitochondria and the secretion of epinephrine was decreased. Two-dimensional gel electrophoresis revealed that among the differentially expressed proteins, 48 protein spots showed an upregulated expression and 37 protein spots showed a downregulated expression, and no ‘all-or-none’ spots with significant differences in expression were found. Fourteen protein spots with an upregulated expression and 6 with a downregulated expression were randomly selected for identification by mass spectrometry. Western blot analysis revealed that ras homologus oncogene (Rho) GDP dissociation inhibitor α (RhoGDIα) protein expression was significantly downregulated and peripherin protein expression was significantly upregulated. In brief, our data demonstrate that NGF can induce the differentiation of adrenal medullary cells into neurons, and that RhoGDIα and peripherin may play important roles in this process.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Proteomic analysis of NGF-induced transdifferentiation of adrenal medullary cells

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