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International Journal of Molecular Medicine
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August 2013 Volume 32 Issue 2

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Article

Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease

  • Authors:
    • Roman Gardlik
    • Anastazie Bartonova
    • Peter Celec
  • View Affiliations / Copyright

    Affiliations: Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, 81108 Bratislava, Slovakia, Clinical and Transplant Pathology Department, Institute for Clinical and Experimental Medicine, 14021 Prague, Czech Republic
  • Pages: 492-496
    |
    Published online on: May 23, 2013
       https://doi.org/10.3892/ijmm.2013.1388
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Abstract

Angiogenesis plays a significant role in numerous diseases. Recently, dysbalanced angiogenesis was found to be a component of pathogenesis of inflammatory bowel disease (IBD). Therefore, inhibition of angiogenesis is a novel therapeutic strategy that alleviates the inflammation and clinical outcomes of IBD. Bacteria act as vectors for the delivery of therapeutic sequences, a process that is particularly suitable in IBD, due to the natural occurrence of bacteria in gut. The main focus of the present study was the application of bacterial gene therapy in the modulation of angiogenesis in IBD. As a target molecule we used the main proangiogenic factor, vascular endothelial growth factor (VEGF). Bacterial strain Salmonella typhimurium SL7207 was used as a gene delivery vector for oral application. DNA vaccination and RNA interference were examined and their efficiency in improving the course of the disease in dextran sulfate sodium-induced colitis in mice was compared. The two approaches yielded similar beneficial results in evaluation of the disease activity parameters (stool consistency, weight loss and colon length) as well as VEGF expression and tumor necrosis factor-α (TNF-α). Improvement in all of the parameters compared with the control groups not treated by therapeutic bacterial strains was also observed. All the bacterial groups showed similar improvement in histopathological scoring. Results of this study are consistent with the literature and provide a basis for additional studies on the modulation of angiogenesis in IBD.
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1 

Kanazawa S, Tsunoda T, Onuma E, Majima T, Kagiyama M and Kikuchi K: VEGF, basic-FGF, and TGF-beta in Crohn’s disease and ulcerative colitis: a novel mechanism of chronic intestinal inflammation. Am J Gastroenterol. 96:822–828. 2001.PubMed/NCBI

2 

Bousvaros A, Leichtner A, Zurakowski D, Kwon J, Law T, Keough K and Fishman S: Elevated serum vascular endothelial growth factor in children and young adults with Crohn’s disease. Dig Dis Sci. 44:424–430. 1999.

3 

Danese S, Sans M, de la Motte C, et al: Angiogenesis as a novel component of inflammatory bowel disease pathogenesis. Gastroenterology. 130:2060–2073. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Goebel S, Huang M, Davis WC, Jennings M, Siahaan TJ, Alexander JS and Kevil CG: VEGF-A stimulation of leukocyte adhesion to colonic microvascular endothelium: implications for inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol. 290:G648–G654. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Mori M, Stokes KY, Vowinkel T, et al: Colonic blood flow responses in experimental colitis: time course and underlying mechanisms. Am J Physiol Gastrointest Liver Physiol. 289:G1024–G1029. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Chidlow JH Jr, Langston W, Greer JJ, et al: Differential angiogenic regulation of experimental colitis. Am J Pathol. 169:2014–2030. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Scaldaferri F, Vetrano S, Sans M, et al: VEGF-A links angiogenesis and inflammation in inflammatory bowel disease pathogenesis. Gastroenterology. 136:585–595. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Chernoguz A, Crawford K, Vandersall A, Rao M, Willson T, Denson LA and Frischer JS: Pretreatment with anti-VEGF therapy may exacerbate inflammation in experimental acute colitis. J Pediatr Surg. 47:347–354. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Palffy R, Behuliak M, Gardlik R, Jáni P, Kadasi L, Turna J and Celec P: Oral in vivo bactofection in dextran sulfate sodium treated female Wistar rats. Folia Biol (Krakow). 58:171–176. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Palffy R, Gardlik R, Behuliak M, et al: Salmonella-mediated gene therapy in experimental colitis in mice. Exp Biol Med (Maywood). 236:177–183. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Gardlik R, Behuliak M, Palffy R, Celec P and Li CJ: Gene therapy for cancer: bacteria-mediated anti-angiogenesis therapy. Gene Ther. 18:425–431. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Darji A, Guzman CA, Gerstel B, et al: Oral somatic transgene vaccination using attenuated S. typhimurium. Cell. 91:765–775. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Dieleman LA, Palmen MJ, Akol H, Bloemena E, Pena AS, Meuwissen SG and Van Rees EP: Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol. 114:385–391. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Lowry OH, Rosebrough NJ, Farr AL and Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem. 193:265–275. 1951.PubMed/NCBI

15 

Paglia P, Medina E, Arioli I, Guzman CA and Colombo MP: Gene transfer in dendritic cells, induced by oral DNA vaccination with Salmonella typhimurium, results in protective immunity against a murine fibrosarcoma. Blood. 92:3172–3176. 1998.PubMed/NCBI

16 

Tolstanova G, Deng X, Khomenko T, et al: Role of anti-angiogenic factor endostatin in the pathogenesis of experimental ulcerative colitis. Life Sci. 88:74–81. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gardlik R, Bartonova A and Celec P: Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease . Int J Mol Med 32: 492-496, 2013.
APA
Gardlik, R., Bartonova, A., & Celec, P. (2013). Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease . International Journal of Molecular Medicine, 32, 492-496. https://doi.org/10.3892/ijmm.2013.1388
MLA
Gardlik, R., Bartonova, A., Celec, P."Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease ". International Journal of Molecular Medicine 32.2 (2013): 492-496.
Chicago
Gardlik, R., Bartonova, A., Celec, P."Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease ". International Journal of Molecular Medicine 32, no. 2 (2013): 492-496. https://doi.org/10.3892/ijmm.2013.1388
Copy and paste a formatted citation
x
Spandidos Publications style
Gardlik R, Bartonova A and Celec P: Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease . Int J Mol Med 32: 492-496, 2013.
APA
Gardlik, R., Bartonova, A., & Celec, P. (2013). Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease . International Journal of Molecular Medicine, 32, 492-496. https://doi.org/10.3892/ijmm.2013.1388
MLA
Gardlik, R., Bartonova, A., Celec, P."Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease ". International Journal of Molecular Medicine 32.2 (2013): 492-496.
Chicago
Gardlik, R., Bartonova, A., Celec, P."Therapeutic DNA vaccination and RNA interference in inflammatory bowel disease ". International Journal of Molecular Medicine 32, no. 2 (2013): 492-496. https://doi.org/10.3892/ijmm.2013.1388
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