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Article

Allosteric regulation of the calcium-sensing receptor in obese individuals

  • Authors:
    • Yonghan He
    • Ben Perry
    • Mingxin Bi
    • Hui Sun
    • Tiantian Zhao
    • Ying Li
    • Changhao Sun
  • View Affiliations / Copyright

    Affiliations: National Key Discipline of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, Prince Edward Island C1A 4P3, Canada, Emergency Department, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Psychology, University of Toronto, Toronto, Ontario M1C 1A4, Canada
  • Pages: 511-518
    |
    Published online on: June 5, 2013
       https://doi.org/10.3892/ijmm.2013.1408
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Abstract

We have previously reported that the calcium-sensing receptor (CaSR) plays an important role in modulating lipid metabolism under low calcium conditions. The aim of this study was to identify possible regulators of CaSR and the mechanisms of action of CaSR in obese individuals. Subcutaneous fat samples were obtained from 10 obese and 10 non-obese males undergoing elective abdominal surgery. Visceral fat pads were obtained from 12 obese and 12 non‑obese male rats. Serum lipid, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations, as well as the gene and protein expression of CaSR in the white adipose tissue of obese subjects and rats were determined. Serum total calcium, vitamin D, parathyroid hormone (PTH) and amino acid levels in human subjects were measured. Intracellular calcium and cyclic adenosine monophosphate (cAMP) levels in rat adipocytes were measured by laser scanning confocal microscopy and ELISA, respectively. The results revealed that serum triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), TNF-α, IL-6 and PTH levels were significantly higher in the obese individuals versus the controls. By contrast, serum vitamin D and amino acid concentrations were lower in the obese individuals versus the controls. In addition, intracellular calcium levels were higher, while cAMP levels were lower in the obese rat adipocytes compared with the control group. However, the gene and protein expression of CaSR in white adipose tissue did not differ between the obese groups and the controls. Thus, these results suggest that CaSR functions not through its expression, but rather through allosteric regulation in obese individuals.
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Copy and paste a formatted citation
Spandidos Publications style
He Y, Perry B, Bi M, Sun H, Zhao T, Li Y and Sun C: Allosteric regulation of the calcium-sensing receptor in obese individuals. Int J Mol Med 32: 511-518, 2013.
APA
He, Y., Perry, B., Bi, M., Sun, H., Zhao, T., Li, Y., & Sun, C. (2013). Allosteric regulation of the calcium-sensing receptor in obese individuals. International Journal of Molecular Medicine, 32, 511-518. https://doi.org/10.3892/ijmm.2013.1408
MLA
He, Y., Perry, B., Bi, M., Sun, H., Zhao, T., Li, Y., Sun, C."Allosteric regulation of the calcium-sensing receptor in obese individuals". International Journal of Molecular Medicine 32.2 (2013): 511-518.
Chicago
He, Y., Perry, B., Bi, M., Sun, H., Zhao, T., Li, Y., Sun, C."Allosteric regulation of the calcium-sensing receptor in obese individuals". International Journal of Molecular Medicine 32, no. 2 (2013): 511-518. https://doi.org/10.3892/ijmm.2013.1408
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Perry B, Bi M, Sun H, Zhao T, Li Y and Sun C: Allosteric regulation of the calcium-sensing receptor in obese individuals. Int J Mol Med 32: 511-518, 2013.
APA
He, Y., Perry, B., Bi, M., Sun, H., Zhao, T., Li, Y., & Sun, C. (2013). Allosteric regulation of the calcium-sensing receptor in obese individuals. International Journal of Molecular Medicine, 32, 511-518. https://doi.org/10.3892/ijmm.2013.1408
MLA
He, Y., Perry, B., Bi, M., Sun, H., Zhao, T., Li, Y., Sun, C."Allosteric regulation of the calcium-sensing receptor in obese individuals". International Journal of Molecular Medicine 32.2 (2013): 511-518.
Chicago
He, Y., Perry, B., Bi, M., Sun, H., Zhao, T., Li, Y., Sun, C."Allosteric regulation of the calcium-sensing receptor in obese individuals". International Journal of Molecular Medicine 32, no. 2 (2013): 511-518. https://doi.org/10.3892/ijmm.2013.1408
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