Induction of apoptosis and the regulation of ErbB signaling by laminarin in HT-29 human colon cancer cells

Park,Hee-Kyoung; Kim,In-Hye; Kim,Joongkyun; Nam,Taek-Jeong

doi:10.3892/ijmm.2013.1409

Induction of apoptosis and the regulation of ErbB signaling by laminarin in HT-29 human colon cancer cells

Authors:
- Hee-Kyoung Park
- In-Hye Kim
- Joongkyun Kim
- Taek-Jeong Nam
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Affiliations: Department of Food and Life Science, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea, Department of Biotechnology, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea
Published online on: June 5, 2013 https://doi.org/10.3892/ijmm.2013.1409
Pages: 291-295
Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it is also used in traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which laminarin from Laminaria digitata induces apoptosis in HT-29 colon cancer cells, as well as the involvement of the ErbB signaling pathway. Cell viability assay revealed that laminarin induced cell death in a dose-dependent manner. Cell cycle analysis revealed that laminarin increased the percentage of cells in the sub-G1 and G2-M phase. Western blot analysis demonstrated that laminarin inhibited the heregulin-stimulated phosphorylation of ErbB2. A decrease in cellular proliferation was also observed; this was found to be dependent on ErbB, which activates c-Jun N-terminal kinase. These findings demonstrate the important role of the epidermal growth factor receptor in colon cancer tumorigenesis, and suggest the potential of laminarin as a bio-functional food with anticancer effects on human colon cancer.

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