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Article

Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis

  • Authors:
    • Shuang Liang
    • Wei-Wei Su
    • Yong-Gang Wang
    • Wei Peng
    • Yi-Chu Nie
    • Pei-Bo Li
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Gene Engineering of the Ministry of Education, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, P.R. China
  • Pages: 323-330
    |
    Published online on: June 11, 2013
       https://doi.org/10.3892/ijmm.2013.1414
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Abstract

Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of Hypericum japonicum. However, whether Q7R is one of the active ingredients responsible for the hepatopreventive effects of Hypericum japonicum has not yet been ascertained. Thus, the aim of the present study was to elucidate whether Q7R attenuates apoptosis induced by glycochenodeoxycholic acid (GCDC) in vitro, and to elucidate the mechanisms involved. L-02 human normal liver cells were pre-incubated with 0, 50, 100 and 200 µM Q7R for 30 min and then exposed to 100 µM GCDC for the indicated periods of time. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was performed to examine cell viability. Apoptosis was evaluated by Hoechst 33258 staining and Annexin V-FITC/PI double staining. Intracellular reactive oxygen species (ROS) were detected by flow cytometry using the oxidation-sensitive fluorescent probe, DCFH-DA. The assay for glutathione (GSH) was performed using a GSH detection kit. Intracellular Ca2+ concentration was evaluated using a confocal laser scanning microscope with Fluo-3 as the Ca2+ probe and mitochondrial membrane potential (Δψm) was measured by rhodamine 123 (Rh123) fluorescence. Q7R attenuated the GCDC-induced reduction in cell viability and the high apoptotic rate. Moreover, Q7R protected the L-02 cells from ROS overproduction, GSH depletion, intracellular Ca2+ accumulation and Δψm decrease induced by GCDC. These results suggest that Q7R attenuates L-02 cell injury induced by GCDC, possibly by inhibiting the overproduction of ROS, GSH depletion, intracellular Ca2+ accumulation and Δψm decrease, thereby minimizing L-02 cell apoptosis.
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1 

Jones BA and Gores GJ: Physiology and pathophysiology of apoptosis in epithelial cells of the liver, pancreas, and intestine. Am J Physiol. 273:G1174–G1188. 1997.PubMed/NCBI

2 

Oh SH, Yun KJ, Nan JX, Sohn DH and Lee BH: Changes in expression and immunolocalization of protein associated with toxic bile salts-induced apoptosis in rat hepatocytes. Arch Toxicol. 77:110–115. 2003.PubMed/NCBI

3 

Rodrigues CM and Steer CJ: Mitochondrial membrane perturbations in cholestasis. J Hepatol. 32:135–141. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Yerushalmi B, Dahl R, Devereaux MW, Gumpricht E and Sokol RJ: Bile acid-induced rat hepatocyte apoptosis is inhibited by antioxidants and blockers of the mitochondrial permeability transition. Hepatology. 33:616–626. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Guicciardi ME and Gores GJ: Apoptosis: a mechanism of acute and chronic liver injury. Gut. 54:1024–1033. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Palmeira CM and Rolo AP: Mitochondrially-mediated toxicity of bile acids. Toxicology. 203:1–15. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Galle PR, Theilmann L, Raedsch R, Otto G and Stiehl A: Ursodeoxycholate reduces hepatotoxicity of bile salts in primary human hepatocytes. Hepatology. 12:486–491. 1990. View Article : Google Scholar : PubMed/NCBI

8 

Perez MJ and Briz O: Bile-acid-induced cell injury and protection. World J Gastroenterol. 15:1677–1689. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Patel T, Bronk SF and Gores GJ: Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes. J Clin Invest. 94:2183–2192. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Kwo P, Patel T, Bronk SF and Gores GJ: Nuclear serine protease activity contributes to bile acid-induced apoptosis in hepatocytes. Am J Physiol. 268:G613–G621. 1995.PubMed/NCBI

11 

Billington D, Evans CE, Godfrey PP and Coleman R: Effects of bile salts on the plasma membranes of isolated rat hepatocytes. Biochem J. 188:321–327. 1980.PubMed/NCBI

12 

Sokol RJ, Straka MS, Dahl R, et al: Role of oxidant stress in the permeability transition induced in rat hepatic mitochondria by hydrophobic bile acids. Pediatr Res. 49:519–531. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Maillette de Buy Wenniger L and Beuers U: Bile salts and cholestasis. Dig Liver Dis. 42:409–418. 2010.PubMed/NCBI

14 

Perez MJ, Macias RI and Marin JJ: Maternal cholestasis induces placental oxidative stress and apoptosis. Protective effect of ursodeoxycholic acid. Placenta. 27:34–41. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Wang N, Li P, Wang Y, et al: Hepatoprotective effect of Hypericum japonicum extract and its fractions. J Ethnopharmacol. 116:1–6. 2008.

16 

Su J, Fu P, Shen Y, et al: Simultaneous analysis of flavonoids from Hypericum japonicum Thunb. ex Murray (Hypericaceae) by HPLC-DAD-ESI/MS. J Pharm Biomed Anal. 46:342–348. 2008.

17 

Choi HJ, Kim JH, Lee CH, Ahn YJ, Song JH, Baek SH and Kwon DH: Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus. Antiviral Res. 81:77–81. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Song JH, Shim JK and Choi HJ: Quercetin 7-rhamnoside reduces porcine epidemic diarrhea virus replication via independent pathway of viral induced reactive oxygen species. Virol J. 8:4602011. View Article : Google Scholar

19 

Awaad AS, Maitland DJ and Soliman GA: Hepatoprotective activity of Schouwia thebica webb. Bioorg Med Chem Lett. 16:4624–4628. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Rathee P, Rathee D, Rathee D and Rathee S: In-vitro cytotoxic activity of β-Sitosterol triacontenate isolated from Capparis decidua (Forsk.) Edgew. Asian Pac J Trop Med. 5:225–230. 2012.

21 

Vermes I, Haanen C, Steffens-Nakken H and Reutelingsperger C: A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 184:39–51. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Hofmann AF: Cholestatic liver disease: pathophysiology and therapeutic options. Liver. 22:14–19. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Rust C, Wild N, Bernt C, Vennegeerts T, Wimmer R and Beuers U: Bile acid-induced apoptosis in hepatocytes is caspase-6-dependent. J Biol Chem. 284:2908–2916. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Lee TY, Chen FY, Chang HH and Lin HC: The effect of capillarisin on glycochenodeoxycholic acid-induced apoptosis and heme oxygenase-1 in rat primary hepatocytes. Mol Cell Biochem. 325:53–59. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Wang K, Brems JJ, Gamelli RL and Holterman AX: Survivin signaling is regulated through nuclear factor-kappa B pathway during glycochenodeoxycholate-induced hepatocyte apoptosis. Biochim Biophys Acta. 1803:1368–1375. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Graf D, Kurz AK, Reinehr R, Fischer R, Kircheis G and Häussinger D: Prevention of bile acid-induced apoptosis by betaine in rat liver. Hepatology. 36:829–839. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Kaplowitz N: Mechanisms of liver cell injury. J Hepatol. 32(Suppl 1): S39–S47. 2000. View Article : Google Scholar

28 

Kim JS, He L and Lemasters JJ: Mitochondrial permeability transition: a common pathway to necrosis and apoptosis. Biochem Biophys Res Commun. 304:463–470. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Sokol RJ, Dahl R, Devereaux MW, Yerushalmi B, Kobak GE and Gumpricht E: Human hepatic mitochondria generate reactive oxygen species and undergo the permeability transition in response to hydrophobic bile acids. J Pediatr Gastroenterol Nutr. 41:235–243. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Sokol RJ, Winklhofer-Roob BM, Devereaux MW and McKim JM Jr: Generation of hydroperoxides in isolated rat hepatocytes and hepatic mitochondria exposed to hydrophobic bile acids. Gastroenterology. 109:1249–1256. 1995. View Article : Google Scholar : PubMed/NCBI

31 

Meister A: Glutathione metabolism and its selective modification. J Biol Chem. 263:17205–17208. 1988.PubMed/NCBI

32 

Fernandes RS and Cotter TG: Apoptosis and necrosis: intracellular levels of glutathione influence mode of cell death. Biochem Pharmacol. 48:675–681. 1994. View Article : Google Scholar : PubMed/NCBI

33 

Ben-Yoseph O, Boxer PA and Ross BD: Assessment of the role of the glutathione and pentose phosphate pathways in the protection of primary cerebrocortical cultures from oxidative stress. J Neurochem. 66:2329–2337. 1996. View Article : Google Scholar : PubMed/NCBI

34 

Scaffidi C, Fulda S, Srinivasan A, et al: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 17:1675–1687. 1998. View Article : Google Scholar : PubMed/NCBI

35 

Venglovecz V, Rakonczay Z Jr, Ozsvári B, et al: Effects of bile acids on pancreatic ductal bicarbonate secretion in guinea pig. Gut. 57:1102–1112. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Anwer MS, Engelking LR, Nolan K, Sullivan D, Zimniak P and Lester R: Hepatotoxic bile acids increase cytosolic Ca2+ activity of isolated rat hepatocytes. Hepatology. 8:887–891. 1988. View Article : Google Scholar : PubMed/NCBI

37 

Berridge MJ: Inositol trisphosphate and calcium signalling mechanisms. Biochim Biophys Acta. 1793:933–940. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liang S, Su W, Wang Y, Peng W, Nie Y and Li P: Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. Int J Mol Med 32: 323-330, 2013.
APA
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., & Li, P. (2013). Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. International Journal of Molecular Medicine, 32, 323-330. https://doi.org/10.3892/ijmm.2013.1414
MLA
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., Li, P."Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis". International Journal of Molecular Medicine 32.2 (2013): 323-330.
Chicago
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., Li, P."Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis". International Journal of Molecular Medicine 32, no. 2 (2013): 323-330. https://doi.org/10.3892/ijmm.2013.1414
Copy and paste a formatted citation
x
Spandidos Publications style
Liang S, Su W, Wang Y, Peng W, Nie Y and Li P: Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. Int J Mol Med 32: 323-330, 2013.
APA
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., & Li, P. (2013). Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. International Journal of Molecular Medicine, 32, 323-330. https://doi.org/10.3892/ijmm.2013.1414
MLA
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., Li, P."Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis". International Journal of Molecular Medicine 32.2 (2013): 323-330.
Chicago
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., Li, P."Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis". International Journal of Molecular Medicine 32, no. 2 (2013): 323-330. https://doi.org/10.3892/ijmm.2013.1414
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