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Article Open Access

Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure

Retraction in: /10.3892/ijmm.2025.5536
  • Authors:
    • Hui Pang
    • Bing Han
    • Tao Yu
    • Zhenkun Zong
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, Central Hospital of Xuzhou, Xuzhou Clinical School of Xuzhou Medical College, Affiliated Hospital of Southeast University, Xuzhou, Jiangsu, P.R. China, Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China
    Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 756-764
    |
    Published online on: July 2, 2014
       https://doi.org/10.3892/ijmm.2014.1829
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Abstract

It is known that apelin has definite protective effects on various cardiovascular diseases; however, the mechanism through which hypertension with heart failure (H-HF) is affected by pyroglutamylated apelin-13 (Pyr-AP13) remain unclear. Thus, in the present study, we investigated the effects of apelin on the cardiac hemodynamics in rats with hypertension and heart failure. In our study, cardiac function, dimensions and histological determination of the fibrosis of rats with two-kidney, one-clip induced hypertension and sham-operated rats were assessed using an echocardiography system and Masson's trichrome. The infusion of either 5% glucose injection (GS) alone or 5% GS containing Pyr-AP13 as a dose, time-matched design on the cardiac hemodynamics in H-HF rats and sham-operated rats was recorded. For the determination of the effects of potential related proteins on cardiac hemodynamics in the H-HF rats, the animals were divided into 5 groups: i) the sham-operated group (n=8); ii) H-HF (n=8); iii) H-HF with infusion of 0.1 µg dose of Pyr-AP13 (n=8) or 5% glucose (GS) (n=8); iv) H-HF with infusion of 1 µg dose of Pyr-AP13 (n=8) or 5% GS (n=8); and v) H-HF with infusion of 10 µg dose of Pyr-AP13 (n=8) or 5% GS (n=8). The concentration of cyclic adenosine 3',5'-monophosphate (cAMP) was determined by ELISA. The expression of membrane and cytosolic proteins was evaluated by western blot analysis. Significant cardiac and perivascular fibrosis was observed in the H-HF rats. Following the infusion of Pyr-AP13, the systolic and diastolic function was significantly improved in the cardiac hemodynamic parameters in the H-HF rats treated with Pyr-AP13. The apelin receptor (APJ), which was activated by the exogenous infusion of Pyr-AP13, was partially recycled from the cytoplasm back to the plasma membrane; however, membrane APJ was eventually downregulated in the H-HF rats treated with Pyr-AP13 compared with the sham-operated group rats. Our findings suggested that a complex was formed after Pyr-AP13 combined with cellular membrane APJ receptor. However, the endogenous downregulation of the APJ receptor results in benefits from the exogenous administration of apelin.
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Copy and paste a formatted citation
Spandidos Publications style
Pang H, Han B, Yu T and Zong Z: Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536. Int J Mol Med 34: 756-764, 2014.
APA
Pang, H., Han, B., Yu, T., & Zong, Z. (2014). Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536. International Journal of Molecular Medicine, 34, 756-764. https://doi.org/10.3892/ijmm.2014.1829
MLA
Pang, H., Han, B., Yu, T., Zong, Z."Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536". International Journal of Molecular Medicine 34.3 (2014): 756-764.
Chicago
Pang, H., Han, B., Yu, T., Zong, Z."Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536". International Journal of Molecular Medicine 34, no. 3 (2014): 756-764. https://doi.org/10.3892/ijmm.2014.1829
Copy and paste a formatted citation
x
Spandidos Publications style
Pang H, Han B, Yu T and Zong Z: Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536. Int J Mol Med 34: 756-764, 2014.
APA
Pang, H., Han, B., Yu, T., & Zong, Z. (2014). Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536. International Journal of Molecular Medicine, 34, 756-764. https://doi.org/10.3892/ijmm.2014.1829
MLA
Pang, H., Han, B., Yu, T., Zong, Z."Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536". International Journal of Molecular Medicine 34.3 (2014): 756-764.
Chicago
Pang, H., Han, B., Yu, T., Zong, Z."Effect of apelin on the cardiac hemodynamics in hypertensive rats with heart failure Retraction in /10.3892/ijmm.2025.5536". International Journal of Molecular Medicine 34, no. 3 (2014): 756-764. https://doi.org/10.3892/ijmm.2014.1829
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