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Article Open Access

Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia

  • Authors:
    • Xueyan Xiong
    • Xiujuan Shi
    • Fengshan Chen
  • View Affiliations / Copyright

    Affiliations: Laboratory of Oral Biomedical Science and Translational Medicine, Department of Orthodontics, School of Stomatology, Tongji University, Shanghai 200072, P.R. China, School of Medicine, Tongji University, Shanghai 200092, P.R. China
    Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 749-755
    |
    Published online on: July 8, 2014
       https://doi.org/10.3892/ijmm.2014.1837
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Abstract

Hyposalivation is an intractable side‑effect of radiotherapy for head and neck cancer. It is caused by the irreversible loss of acinar cells and decreased saliva secretion. However, this situation severely compromises the quality of life of affected patients. Currently, there is no effective treatment for this condition. In the present study, we developed a novel approach to regenerate the function of the irradiation‑damaged salivary glands using human adipose tissue‑derived stem cell (hADSC) intraglandular transplantation. ZsGreen‑labeled hADSCs were adoptively transferred into Sprague‑Dawley (SD) rat submandibular glands immediately following exposure to 18 Gy irradiation. A higher salivary flow rate (SFR) was observed in the hADSC‑treated group. Tissue improvement, including angiogenesis, anti‑apoptosis and anti‑fibrosis, was detected in the hADSC‑treated glands as compared to the untreated glands. Quantitative reverse transcription PCR (RT-qPCR) revealed a significantly higher expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), cyclooxygenase‑2 (COX‑2) and matrix metalloproteinase‑2 (MMP‑2) in the hADSC‑treated rats. Furthermore, immunohistochemical analysis indicated that the hADSCs had differentiated into acinar and ductal cells in the rat submandibular glands. Thus, our results suggest that hADSCs are able to regenerate irradiation‑damaged salivary glands through glandular transplantation.
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1 

Baum BJ and Tran SD: Synergy between genetic and tissue engineering: creating an artificial salivary gland. Periodontol 2000. 41:218–223. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Vissink A, Jansma J, Spijkervet FK, Burlage FR and Coppes RP: Oral sequelae of head and neck radiotherapy. Crit Rev Oral Biol Med. 14:199–212. 2003. View Article : Google Scholar

3 

Konings AW, Coppes RP and Vissink A: On the mechanism of salivary gland radiosensitivity. Int J Radiat Oncol Biol Phys. 62:1187–1194. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Nieuw Amerongen AV and Veerman EC: Current therapies for xerostomia and salivary gland hypofunction associated with cancer therapies. Support Care Cancer. 11:226–231. 2003.PubMed/NCBI

5 

Lombaert IM, Wierenga PK, Kok T, Kampinga HH, deHaan G and Coppes RP: Mobilization of bone marrow stem cells by granulocyte colony-stimulating factor ameliorates radiation-induced damage to salivary glands. Clin Cancer Res. 12:1804–1812. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Sumita Y, Liu Y, Khalili S, Maria OM, Xia D, Key S, Cotrim AP, Mezey E and Tran SD: Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation. Int J Biochem Cell Biol. 43:80–87. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Lombaert IM, Brunsting JF, Wierenga PK, Faber H, Stokman MA, Kok T, Visser WH, Kampinga HH, de Haan G and Coppes RP: Rescue of salivary gland function after stem cell transplantation in irradiated glands. PloS One. 3:e20632008. View Article : Google Scholar : PubMed/NCBI

8 

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP and Hedrick MH: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Schäffler A and Büchler C: Concise review: adipose tissue-derived stromal cells-basic and clinical implications for novel cell-based therapies. Stem Cells. 25:818–827. 2007.PubMed/NCBI

10 

Wen Z, Liao Q, Hu Y, Liu S, You L and Zhao Y: Human adipose-derived stromal/stem cells: a novel approach to inhibiting acute pancreatitis. Med Hypotheses. 80:598–600. 2013. View Article : Google Scholar

11 

Kim JM, Lee ST, Chu K, Jung KH, Song EC, Kim SJ, Sinn DI, Kim JH, Park DK, Kang KM, et al: Systemic transplantation of human adipose stem cells attenuated cerebral inflammation and degeneration in a hemorrhagic stroke model. Brain Res. 1183:43–50. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Ra JC, Shin IS, Kim SH, Kang SK, Kang BC, Lee HY, Kim YJ, Jo JY, Yoon EJ, Choi HJ and Kwon E: Safety of intravenous infusion of human adipose tissue-derived mesenchymal stem cells in animals and humans. Stem Cells Dev. 20:1297–1308. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Chen G, Shi X, Sun C, Li M, Zhou Q, Zhang C, Huang J, Qiu Y, Wen X, Zhang Y, et al: VEGF-mediated proliferation of human adipose tissue-derived stem cells. PloS One. 8:e736732013. View Article : Google Scholar : PubMed/NCBI

14 

Labat-Moleur F, Guillermet C, Lorimier P, Robert C, Lantuejoul S, Brambilla E and Negoescu A: TUNEL apoptotic cell detection in tissue sections: critical evaluation and improvement. J Histochem Cytochem. 46:327–334. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, Pell CL, Johnstone BH, Considine RV and March KL: Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 109:1292–1298. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Friedenstein AJ, Chailakhjan RK and Lalykina KS: The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 3:393–403. 1970.PubMed/NCBI

17 

Erices A, Conget P and Minguell JJ: Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 109:235–242. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Zuk PA, Zhu M, Ashjian P, et al: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002.PubMed/NCBI

19 

Karp JM and Leng Teo GS: Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell. 4:206–216. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Freyman T, Polin G, Osman H, Crary J, Lu M, Cheng L, Palasis M and Wilensky RL: A quantitative, randomized study evaluating three methods of mesenchymal stem cell delivery following myocardial infarction. Eur Heart J. 27:1114–1122. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Furlani D, Ugurlucan M, Ong L, Bieback K, Pittermann E, Westien I, Wang W, Yerebakan C, Li W, Gaebel R, et al: Is the intravascular administration of mesenchymal stem cells safe? Mesenchymal stem cells and intravital microscopy. Microvasc Res. 77:370–376. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Rodriguez AM, Pisani D, Dechesne CA, Turc-Carel C, Kurzenne JY, Wdziekonski B, Villageois A, Bagnis C, Breittmayer JP, Groux H, Ailhaud G and Dani C: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse. J Exp Med. 201:1397–1405. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Saarilahti K, Kouri M, Collan J, Kangasmäki A, Atula T, Joensuu H and Tenhunen M: Sparing of the submandibular glands by intensity modulated radiotherapy in the treatment of head and neck cancer. Radiother Oncol. 78:270–275. 2006. View Article : Google Scholar

24 

Nakagami H, Morishita R, Maeda K, Kikuchi Y, Ogihara T and Kaneda Y: Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy. J Atheroscler Thromb. 13:77–81. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Lim J-Y, Ra JC, Choi J-S, Jang YH, Lee S, Kim WC and Kim Y-M: OP061: Adipose tissue-derived mesenchymal stem cells for regeneration of irradiation-induced salivary gland damage. Oral Oncology. 49(Suppl 1): S28–S29. 2013. View Article : Google Scholar

26 

Baum BJ: Principles of saliva secretion. Ann N Y Acad Sci. 694:17–23. 1993. View Article : Google Scholar : PubMed/NCBI

27 

Saito T, Kuang JQ, Bittira B, Al-Khaldi A and Chiu RC: Xenotransplant cardiac chimera: immune tolerance of adult stem cells. Ann Thorac Surg. 74:19–24. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Ryan JM, Barry FP, Murphy JM and Mahon BP: Mesenchymal stem cells avoid allogeneic rejection. J Inflamm (Lond). 2:82005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xiong X, Shi X and Chen F: Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia. Int J Mol Med 34: 749-755, 2014.
APA
Xiong, X., Shi, X., & Chen, F. (2014). Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia. International Journal of Molecular Medicine, 34, 749-755. https://doi.org/10.3892/ijmm.2014.1837
MLA
Xiong, X., Shi, X., Chen, F."Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia". International Journal of Molecular Medicine 34.3 (2014): 749-755.
Chicago
Xiong, X., Shi, X., Chen, F."Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia". International Journal of Molecular Medicine 34, no. 3 (2014): 749-755. https://doi.org/10.3892/ijmm.2014.1837
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong X, Shi X and Chen F: Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia. Int J Mol Med 34: 749-755, 2014.
APA
Xiong, X., Shi, X., & Chen, F. (2014). Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia. International Journal of Molecular Medicine, 34, 749-755. https://doi.org/10.3892/ijmm.2014.1837
MLA
Xiong, X., Shi, X., Chen, F."Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia". International Journal of Molecular Medicine 34.3 (2014): 749-755.
Chicago
Xiong, X., Shi, X., Chen, F."Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia". International Journal of Molecular Medicine 34, no. 3 (2014): 749-755. https://doi.org/10.3892/ijmm.2014.1837
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