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Article

Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production

  • Authors:
    • Jian‑Zhe Li
    • Shu‑Yi Yu
    • Dan Mo
    • Xiu‑Neng Tang
    • Qing‑Rui Shao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning, Guangxi 530011, P.R. China, Modern Analysis and Testing Center, Central South University, Changsha, Hunan 410078, P.R. China, Department of Surgery, Maternal and Child Health Hospital of the Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530003, P.R. China
  • Pages: 446-452
    |
    Published online on: November 25, 2014
       https://doi.org/10.3892/ijmm.2014.2009
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Abstract

Reactive oxygen species (ROS)‑induced mitochondrial dysfunction plays an important role in cardiomyocyte apoptosis during myocardial ischemia/reperfusion (I/R) injury. Picroside Ⅱ, isolated from Picrorhiza scrophulariiflora Pennell (Scrophulariaceae), has been reported to protect cardiomyocytes from hypoxia/reoxygenation (H/R)‑induced apoptosis, but the exact mechanism is not fully clear. The aim of the present study was to explore the protective effects of picroside Ⅱ on H/R‑induced cardiomyocyte apoptosis and the underlying mechanism. In the H9c2 rat cardiomyocyte cell line, picroside Ⅱ (100 µg/ml) was added for 48 h prior to H/R. The results showed that picroside Ⅱ markedly inhibited H/R‑induced cardiomyocyte apoptosis. In addition, picroside Ⅱ was also able to decrease the opening degree of mitochondrial permeability transition pore (mPTP), increase the mitochondrial membrane potential, inhibit cytochrome c release from mitochondria to cytosol and downregulate caspase‑3 expression and activity concomitantly with the decreased ROS production. These results suggested that picroside Ⅱ inhibited H/R‑induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in ROS production.
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Copy and paste a formatted citation
Spandidos Publications style
Li JZ, Yu SY, Mo D, Tang XN and Shao QR: Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production. Int J Mol Med 35: 446-452, 2015.
APA
Li, J., Yu, S., Mo, D., Tang, X., & Shao, Q. (2015). Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production. International Journal of Molecular Medicine, 35, 446-452. https://doi.org/10.3892/ijmm.2014.2009
MLA
Li, J., Yu, S., Mo, D., Tang, X., Shao, Q."Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production". International Journal of Molecular Medicine 35.2 (2015): 446-452.
Chicago
Li, J., Yu, S., Mo, D., Tang, X., Shao, Q."Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production". International Journal of Molecular Medicine 35, no. 2 (2015): 446-452. https://doi.org/10.3892/ijmm.2014.2009
Copy and paste a formatted citation
x
Spandidos Publications style
Li JZ, Yu SY, Mo D, Tang XN and Shao QR: Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production. Int J Mol Med 35: 446-452, 2015.
APA
Li, J., Yu, S., Mo, D., Tang, X., & Shao, Q. (2015). Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production. International Journal of Molecular Medicine, 35, 446-452. https://doi.org/10.3892/ijmm.2014.2009
MLA
Li, J., Yu, S., Mo, D., Tang, X., Shao, Q."Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production". International Journal of Molecular Medicine 35.2 (2015): 446-452.
Chicago
Li, J., Yu, S., Mo, D., Tang, X., Shao, Q."Picroside Ⅱ inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production". International Journal of Molecular Medicine 35, no. 2 (2015): 446-452. https://doi.org/10.3892/ijmm.2014.2009
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