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Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy

  • Authors:
    • Lei Zhang
    • Shen Qu
    • Aibin Liang
    • Hong Jiang
    • Hao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Special Needs Medical Branch, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, P.R. China, Department of Endocrinology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China, Department of Hematology, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, P.R. China, Department of Radiology, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 333-339
    |
    Published online on: November 26, 2014
       https://doi.org/10.3892/ijmm.2014.2011
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Abstract

The present study aimed to explore novel target genes that regulate the development of diabetic neuropathy (DN) by analyzing gene expression profiles in the sciatic nerve of infected mice. The GSE11343 microarray dataset, which was downloaded from Gene Expression Omnibus, included data on 4 control samples and 5 samples from mice with diabetes induced by streptozotocin (STZ), 5 samples from normal mice treated with rosiglitazone (Rosi) and 5 samples from mice with diabetes induced by STZ and treated with Rosi. Differentially expressed genes (DEGs) between the different groups were identified using the substitution augmentation modification redefinition (SAMR) model. The Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID). Regulatory and protein‑protein interaction networks were searched using BioCarta and STRING, respectively. The protein structures of potential regulatory genes were predicted using the SYBYL program. Compared with the controls, 1,384 DEGs were identified in the mice with STZ-induced diabetes and 7 DEGs were identified in the mice treated with Rosi. There were 518 DEGs identified between the mice in the STZ + Rosi and STZ groups. We identified 45 GO items, and the calmodulin nerve phosphatase and chemokine signaling pathways were identified as the main pathways. Three genes [myristoylated alanine-rich protein kinase C substrate (Marcks), GLI pathogenesis-related 2 (Glipr2) and centrosomal protein 170 kDa (Cep170)] were found to be co-regulated by both STZ and Rosi, the protein structure of which was predicted and certain binding activity to Rosi was docked. Our study demonstrates that the Marcks, Glipr2 and Cep170 genes may be underlying drug targets in the treatment of DN.
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1 

Workowski KA and Berman SM: Centers for Disease Control and Prevention sexually transmitted diseases treatment guidelines. Clin Infect Dis. 44(Suppl 3): S73–S76. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Skljarevski V and Malik RA: Clinical diagnosis of diabetic neuropathy. Diabetic Neuropathy. Veves A and Malik R: 2nd edition. Springer; New Jersey: pp. 275–292. 2007, View Article : Google Scholar

3 

Said G, Goulon-Goeau C, Slama G and Tchobroutsky G: Severe early-onset polyneuropathy in insulin-dependent diabetes mellitus. A clinical and pathological study. N Engl J Med. 326:1257–1263. 1992. View Article : Google Scholar : PubMed/NCBI

4 

Soumyanath A, Dimitrova D, Arnold G, et al: P02.186. CAST (Centella asiatica selected triterpenes): stability, safety, and effect on diabetic neuropathy (DN). BMC Complement Altern Med. 12(Suppl 1): P2422012. View Article : Google Scholar :

5 

Diep QN, El Mabrouk M, Cohn JS, et al: Structure, endothelial function, cell growth, and inflammation in blood vessels of angiotensin II-infused rats role of peroxisome proliferator-activated receptor-gamma. Circulation. 105:2296–2302. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Lygate CA, Hulbert K, Monfared M, Cole MA, Clarke K and Neubauer S: The PPARγ-activator rosiglitazone does not alter remodeling but increases mortality in rats post-myocardial infarction. Cardiovasc Res. 58:632–637. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Szkudelski T: The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 50:537–546. 2001.

8 

Yamamoto H, Shimoshige Y, Yamaji T, Murai N, Aoki T and Matsuoka N: Pharmacological characterization of standard analgesics on mechanical allodynia in streptozotocin-induced diabetic rats. Neuropharmacology. 57:403–408. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Edgar R, Domrachev M and Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 30:207–210. 2002. View Article : Google Scholar :

10 

Tusher VG, Tibshirani R and Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA. 98:5116–5121. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Kanehisa M: The KEGG database. Novartis Found Symp. 247:91–103. 119–128. 244–252. 2002. View Article : Google Scholar

12 

Dennis G Jr, Sherman BT, Hosack DA, et al: DAVID: database for annotation, visualization, and integrated discovery. Genome Biol. 4:P32003. View Article : Google Scholar : PubMed/NCBI

13 

Nishimura D: BioCarta. Biotech Software and Internet Report. 2:117–120. 2001. View Article : Google Scholar

14 

Franceschini A, Szklarczyk D, Frankild S, et al: STRING v9.1: protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res. 41:D808–D815. 2013. View Article : Google Scholar :

15 

Berman HM, Westbrook J, Feng Z, et al: The Protein Data Bank. Nucleic Acids Res. 28:235–242. 2000. View Article : Google Scholar

16 

Bairoch A, Apweiler R, Wu CH, et al: The universal protein resource (UniProt). Nucleic Acids Res. 33:D154–D159. 2005. View Article : Google Scholar :

17 

Altschul SF, Madden TL, Schäffer AA, et al: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389–3402. 1997. View Article : Google Scholar : PubMed/NCBI

18 

Yue TI TL, Chen J, Bao W, et al: In vivo myocardial protection from ischemia/reperfusion injury by the peroxisome proliferator-activated receptor-γ agonist rosiglitazone. Circulation. 104:2588–2594. 2001. View Article : Google Scholar

19 

Hussein Z, Wentworth JM, Nankervis AJ, et al: Effectiveness and side effects of thiazolidinediones for type 2 diabetes: real-life experience from a tertiary hospital. Med J Aust. 181:536–539. 2004.PubMed/NCBI

20 

Vanopdenbosch N, Cramer R and Giarrusso FF: Sybyl, the Integrated Molecular Modeling System. J Mol Graph. 3:110–111. 1985.

21 

Morris GM, Huey R, Lindstrom W, et al: AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. J Comput Chem. 30:2785–2791. 2009. View Article : Google Scholar : PubMed/NCBI

22 

De Groot M, Anderson R, Freedland KE, Clouse RE and Lustman PJ: Association of depression and diabetes complications: a meta-analysis. Psychosom Med. 63:619–630. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Price SA, Zeef LaH, Wardleworth L, Hayes A and Tomlinson DR: Identification of changes in gene expression in dorsal root ganglia in diabetic neuropathy: correlation with functional deficits. J Neuropathol Exp Neur. 65:722–732. 2006. View Article : Google Scholar

24 

Guarguaglini G, Duncan PI, Stierhof YD, Holmström T, Duensing S and Nigg EA: The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles. Mol Biol Cell. 16:1095–1107. 2005. View Article : Google Scholar :

25 

Laux T, Fukami K, Thelen M, Golub T, Frey D and Caroni P: GAP43, MARCKS, and CAP23 modulate PI(4,5)P(2) at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism. J Cell Biol. 149:1455–1472. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Hooi LS, Wong HS and Morad Z: Prevention of renal failure: The Malaysian experience. Kidney Int Suppl. 67:S70–S74. 2005. View Article : Google Scholar

27 

Lokman FE, Seman NA, Al-Safi Ismail A, et al: Gene expression profiling in ethnic Malays with type 2 diabetes mellitus, with and without diabetic nephropathy. J Nephrol. 24:778–789. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Huang S, Liu F, Niu Q, et al: GLIPR-2 overexpression in HK-2 cells promotes cell EMT and migration through ERK1/2 activation. PLoS One. 8:e585742013. View Article : Google Scholar : PubMed/NCBI

29 

Krentz A: Thiazolidinediones: effects on the development and progression of type 2 diabetes and associated vascular complications. Diabetes Metab Res Rev. 25:112–126. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Qu S, Liang A, Jiang H and Wang H: Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy. Int J Mol Med 35: 333-339, 2015.
APA
Zhang, L., Qu, S., Liang, A., Jiang, H., & Wang, H. (2015). Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy. International Journal of Molecular Medicine, 35, 333-339. https://doi.org/10.3892/ijmm.2014.2011
MLA
Zhang, L., Qu, S., Liang, A., Jiang, H., Wang, H."Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy". International Journal of Molecular Medicine 35.2 (2015): 333-339.
Chicago
Zhang, L., Qu, S., Liang, A., Jiang, H., Wang, H."Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy". International Journal of Molecular Medicine 35, no. 2 (2015): 333-339. https://doi.org/10.3892/ijmm.2014.2011
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Qu S, Liang A, Jiang H and Wang H: Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy. Int J Mol Med 35: 333-339, 2015.
APA
Zhang, L., Qu, S., Liang, A., Jiang, H., & Wang, H. (2015). Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy. International Journal of Molecular Medicine, 35, 333-339. https://doi.org/10.3892/ijmm.2014.2011
MLA
Zhang, L., Qu, S., Liang, A., Jiang, H., Wang, H."Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy". International Journal of Molecular Medicine 35.2 (2015): 333-339.
Chicago
Zhang, L., Qu, S., Liang, A., Jiang, H., Wang, H."Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy". International Journal of Molecular Medicine 35, no. 2 (2015): 333-339. https://doi.org/10.3892/ijmm.2014.2011
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