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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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February-2015 Volume 35 Issue 2

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Article

Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1

  • Authors:
    • Ji Sook Kang
    • Min Ho Han
    • Gi‑Young Kim
    • Cheol Min Kim
    • Hae Young Chung
    • Hye Jin Hwang
    • Byung Woo Kim
    • Yung Hyun Choi
  • View Affiliations / Copyright

    Affiliations: Blue‑Bio Industry RIC and Anti‑Aging Research Center, Dongeui University, Busan 614-714, Republic of Korea, Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 614-052, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Department of Biochemistry, Busan National University College of Medicine, Yangsan 626-870, Republic of Korea, Molecular Inflammation Research Center for Aging Intervention (MRCA), Department of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea
  • Pages: 453-459
    |
    Published online on: December 8, 2014
       https://doi.org/10.3892/ijmm.2014.2028
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Abstract

The aim of the present study was to examine the cytoprotective effects of Schisandrae semen essential oil (SSeo), purified from Schisandrae fructus, against oxidative stress‑induced cell damage in C2C12 myoblasts. SSeo attenuated hydrogen peroxide (H2O2)‑induced growth inhibition and exhibited scavenging activity against the intracellular reactive oxygen species (ROS) that were induced by H2O2. SSeo also inhibited comet tail formation, chromatin condensation and phospho‑histone γH2A.X expression, suggesting that it prevents H2O2‑induced cellular DNA damage and apoptotic cell death. Furthermore, SSeo significantly enhanced the expression of heme oxygenase‑1 (HO‑1) associated with the induction of nuclear factor erythroid‑2‑related factor 2 (Nrf2) in a time‑ and concentration‑dependent manner. In addition, the protective effect of SSeo on H2O2‑induced C2C12 cell damage was significantly inhibited by zinc protoporphyrin IX, an HO‑1 competitive inhibitor, in C2C12 cells. These findings suggest that SSeo augments the cellular antioxidant defense capacity through intrinsic free radical scavenging activity and activation of the Nrf2/HO‑1 pathway, thereby protecting the C2C12 cells from H2O2‑induced oxidative cytotoxicity. As a result, SSeo may have therapeutic potential in the development of functional foods and as the raw material for medicines to protect against oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Kang JS, Han MH, Kim GY, Kim CM, Chung HY, Hwang HJ, Kim BW and Choi YH: Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1. Int J Mol Med 35: 453-459, 2015.
APA
Kang, J.S., Han, M.H., Kim, G., Kim, C.M., Chung, H.Y., Hwang, H.J. ... Choi, Y.H. (2015). Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1. International Journal of Molecular Medicine, 35, 453-459. https://doi.org/10.3892/ijmm.2014.2028
MLA
Kang, J. S., Han, M. H., Kim, G., Kim, C. M., Chung, H. Y., Hwang, H. J., Kim, B. W., Choi, Y. H."Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1". International Journal of Molecular Medicine 35.2 (2015): 453-459.
Chicago
Kang, J. S., Han, M. H., Kim, G., Kim, C. M., Chung, H. Y., Hwang, H. J., Kim, B. W., Choi, Y. H."Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1". International Journal of Molecular Medicine 35, no. 2 (2015): 453-459. https://doi.org/10.3892/ijmm.2014.2028
Copy and paste a formatted citation
x
Spandidos Publications style
Kang JS, Han MH, Kim GY, Kim CM, Chung HY, Hwang HJ, Kim BW and Choi YH: Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1. Int J Mol Med 35: 453-459, 2015.
APA
Kang, J.S., Han, M.H., Kim, G., Kim, C.M., Chung, H.Y., Hwang, H.J. ... Choi, Y.H. (2015). Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1. International Journal of Molecular Medicine, 35, 453-459. https://doi.org/10.3892/ijmm.2014.2028
MLA
Kang, J. S., Han, M. H., Kim, G., Kim, C. M., Chung, H. Y., Hwang, H. J., Kim, B. W., Choi, Y. H."Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1". International Journal of Molecular Medicine 35.2 (2015): 453-459.
Chicago
Kang, J. S., Han, M. H., Kim, G., Kim, C. M., Chung, H. Y., Hwang, H. J., Kim, B. W., Choi, Y. H."Schisandrae semen essential oil attenuates oxidative stress-induced cell damage in C2C12 murine skeletal muscle cells through Nrf2‑mediated upregulation of HO‑1". International Journal of Molecular Medicine 35, no. 2 (2015): 453-459. https://doi.org/10.3892/ijmm.2014.2028
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