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International Journal of Molecular Medicine
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Article

Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri)

  • Authors:
    • Yu Zheng
    • Chenxia Yun
    • Qihui Wang
    • Wanli W. Smith
    • Jing Leng
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology and Immunology, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China, Department of Immunology, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD 21201, USA
  • Pages: 519-524
    |
    Published online on: December 15, 2014
       https://doi.org/10.3892/ijmm.2014.2040
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Abstract

The tree shrew (Tupaia belangeri) diverges from the primate order (Primates) and is classified as a separate taxonomic group of mammals - Scandentia. It has been suggested that the tree shrew can be used as an animal model for studying human diseases; however, the genomic sequence of the tree shrew is largely unidentified. In the present study, we reported the full-length cDNA sequence of the housekeeping gene, β-actin, in the tree shrew. The amino acid sequence of β-actin in the tree shrew was compared to that of humans and other species; a simple phylogenetic relationship was discovered. Quantitative polymerase chain reaction (qPCR) and western blot analysis further demonstrated that the expression profiles of β-actin, as a general conservative housekeeping gene, in the tree shrew were similar to those in humans, although the expression levels varied among different types of tissue in the tree shrew. Our data provide evidence that the tree shrew has a close phylogenetic association with humans. These findings further enhance the potential that the tree shrew, as a species, may be used as an animal model for studying human disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng Y, Yun C, Wang Q, Smith WW and Leng J: Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri). Int J Mol Med 35: 519-524, 2015.
APA
Zheng, Y., Yun, C., Wang, Q., Smith, W.W., & Leng, J. (2015). Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri). International Journal of Molecular Medicine, 35, 519-524. https://doi.org/10.3892/ijmm.2014.2040
MLA
Zheng, Y., Yun, C., Wang, Q., Smith, W. W., Leng, J."Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri)". International Journal of Molecular Medicine 35.2 (2015): 519-524.
Chicago
Zheng, Y., Yun, C., Wang, Q., Smith, W. W., Leng, J."Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri)". International Journal of Molecular Medicine 35, no. 2 (2015): 519-524. https://doi.org/10.3892/ijmm.2014.2040
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Y, Yun C, Wang Q, Smith WW and Leng J: Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri). Int J Mol Med 35: 519-524, 2015.
APA
Zheng, Y., Yun, C., Wang, Q., Smith, W.W., & Leng, J. (2015). Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri). International Journal of Molecular Medicine, 35, 519-524. https://doi.org/10.3892/ijmm.2014.2040
MLA
Zheng, Y., Yun, C., Wang, Q., Smith, W. W., Leng, J."Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri)". International Journal of Molecular Medicine 35.2 (2015): 519-524.
Chicago
Zheng, Y., Yun, C., Wang, Q., Smith, W. W., Leng, J."Identification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri)". International Journal of Molecular Medicine 35, no. 2 (2015): 519-524. https://doi.org/10.3892/ijmm.2014.2040
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