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MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells

  • Authors:
    • Shanshan Deng
    • Yanli Zhang
    • Chundi Xu
    • Duan Ma
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 355-362
    |
    Published online on: June 8, 2015
       https://doi.org/10.3892/ijmm.2015.2238
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Abstract

microRNAs (miRNAs or miRS) have been demonstrated to be essential for neural development. miR-125b-2, presented on human chromosome 21, is overexpressed in neurons of individuals with Down syndrome (DS) with cognitive impairments. It has been reported that miR-125b-2 promotes specific types of neuronal differentiation; however, the function of miR-125b-2 in the early development of the embryo has remained to be fully elucidated. In the present study, a mouse embryonic stem cell (mESC) line was stably transfected with a miR-125b-2 lentiviral expression vector and found that miR-125b-2 overexpression did not affect the self-renewal or proliferation of mESCs. However, miR-125b-2 overexpression inhibited the differentiation of mESCs into endoderm and ectoderm. Finally, miR-125b-2 overexpression was found to impair all-trans-retinoic acid-induced neuron development in embryoid bodies. The findings of the present study implied that miR-125b-2 overexpression suppressed the differentiation of mESCs into neurons, which highlights that miR‑125b-2 is important in the regulation of ESC differentiation. The present study provided a basis for the further identification of novel targets of miR-125b-2, which may contribute to an enhanced understanding of the molecular mechanisms of ESC differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Deng S, Zhang Y, Xu C and Ma D: MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells. Int J Mol Med 36: 355-362, 2015.
APA
Deng, S., Zhang, Y., Xu, C., & Ma, D. (2015). MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells. International Journal of Molecular Medicine, 36, 355-362. https://doi.org/10.3892/ijmm.2015.2238
MLA
Deng, S., Zhang, Y., Xu, C., Ma, D."MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells". International Journal of Molecular Medicine 36.2 (2015): 355-362.
Chicago
Deng, S., Zhang, Y., Xu, C., Ma, D."MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells". International Journal of Molecular Medicine 36, no. 2 (2015): 355-362. https://doi.org/10.3892/ijmm.2015.2238
Copy and paste a formatted citation
x
Spandidos Publications style
Deng S, Zhang Y, Xu C and Ma D: MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells. Int J Mol Med 36: 355-362, 2015.
APA
Deng, S., Zhang, Y., Xu, C., & Ma, D. (2015). MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells. International Journal of Molecular Medicine, 36, 355-362. https://doi.org/10.3892/ijmm.2015.2238
MLA
Deng, S., Zhang, Y., Xu, C., Ma, D."MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells". International Journal of Molecular Medicine 36.2 (2015): 355-362.
Chicago
Deng, S., Zhang, Y., Xu, C., Ma, D."MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells". International Journal of Molecular Medicine 36, no. 2 (2015): 355-362. https://doi.org/10.3892/ijmm.2015.2238
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