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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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August-2015 Volume 36 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin

  • Authors:
    • Haipeng Zhu
    • Feng Yun
    • Xiaoxue Shi
    • Dong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China
  • Pages: 595-601
    |
    Published online on: June 17, 2015
       https://doi.org/10.3892/ijmm.2015.2250
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Abstract

The present study aimed to reveal the association between insulin-like growth factor binding protein-2 (IGFBP-2) and the sensitivity of bladder cancer cells to cisplatin, and determine the underlying mechanism involving maspin. A total of 32 bladder cancer tissue samples were collected for analysis. Cells of the BIU87 human bladder cancer cell line were cultured and a cisplatin‑resistant subline (BIU87‑CisR) was established by continuous exposure of the cells to cisplatin. Targeted inhibition of IGFBP‑2 in the BIU87‑CisR cells was performed using small interfering RNA technology. The expression levels of IGFBP‑2 and maspin in the tissue samples and cells were analyzed using reverse transcription‑quantitative polymerase chain reaction and western blot analyses. Cell viability following treatment in each group was evaluated using a Cell Counting Kit‑8 assay subsequent to treatment with 3 µM cisplatin. The cell cycle and apoptotic rate of the BIU87‑CisR cells were analyzed using flow cytometry. Finally, maspin‑overexpressing BIU87‑CisR cells were used to confirm the effect of maspin on the sensitivity of the cells to cisplatin. The expression levels of IGFBP‑2 in chemoresistant patients and BIU87‑CisR cells were significantly increased, compared with those in the chemosensitive patients and BIU87 cells, respectively. However, the expression levels of maspin were lower in the cisplatin‑resistant tissue and cells, and was enhanced by IGFBP‑2 inhibition. Cisplatin (3 µM) caused marked proliferation inhibition, cell cycle arrest and apoptosis of the BIU87‑CisR cells, the effect of which was enhanced by IGFBP‑2 silencing. Overexpression of maspin also improved the sensitivity of the BIU87‑CisR cells to cisplatin. In conclusion, inhibition of IGFBP‑2 improved the sensitivity of bladder cancer cells to cisplatin by elevating the expression of maspin.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu H, Yun F, Shi X and Wang D: Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin. Int J Mol Med 36: 595-601, 2015.
APA
Zhu, H., Yun, F., Shi, X., & Wang, D. (2015). Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin. International Journal of Molecular Medicine, 36, 595-601. https://doi.org/10.3892/ijmm.2015.2250
MLA
Zhu, H., Yun, F., Shi, X., Wang, D."Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin". International Journal of Molecular Medicine 36.2 (2015): 595-601.
Chicago
Zhu, H., Yun, F., Shi, X., Wang, D."Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin". International Journal of Molecular Medicine 36, no. 2 (2015): 595-601. https://doi.org/10.3892/ijmm.2015.2250
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu H, Yun F, Shi X and Wang D: Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin. Int J Mol Med 36: 595-601, 2015.
APA
Zhu, H., Yun, F., Shi, X., & Wang, D. (2015). Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin. International Journal of Molecular Medicine, 36, 595-601. https://doi.org/10.3892/ijmm.2015.2250
MLA
Zhu, H., Yun, F., Shi, X., Wang, D."Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin". International Journal of Molecular Medicine 36.2 (2015): 595-601.
Chicago
Zhu, H., Yun, F., Shi, X., Wang, D."Inhibition of IGFBP-2 improves the sensitivity of bladder cancer cells to cisplatin via upregulating the expression of maspin". International Journal of Molecular Medicine 36, no. 2 (2015): 595-601. https://doi.org/10.3892/ijmm.2015.2250
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