Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections

  • Authors:
    • Xingxing Li
    • Qinlu Zhang
    • Peng Hou
    • Mingwei Chen
    • Wenli Hui
    • Alphons Vermorken
    • Zhiyi Luo
    • Hong Li
    • Qin Li
    • Yali Cui
  • View Affiliations

  • Published online on: September 2, 2015     https://doi.org/10.3892/ijmm.2015.2333
  • Pages: 1319-1326
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Abstract

In this study, a lateral flow immunochromatographic assay (LFIA) system for the detection of immunoglobulin M (IgM) antibodies, related to TORCH [(T)oxoplasmosis, (O)ther agents, (R)ubella (also known as German Measles), (C)ytomegalovirus, and (H)erpes simplex virus infections], based on gold magnetic nanoparticles, was established. Following modification with poly(methacrylic acid), the gold magnetic nanoparticles conjugated with an anti‑human IgM antibody (μ‑chain specific) to construct a probe. A lateral flow assay device was constructed based on these conjugates. IgM antibodies to four types of pathogens, notably toxoplasmosis, rubella virus, cytomegalovirus and herpes simplex virus type 2, were detected using this device. Compared with commercial colloidal gold‑based LFIA strips, our method exhibited higher sensitivity. No interference with triglycerides, hemoglobin and bilirubin occurred, and no cross‑reactivity was noted among the four pathogens. The gold magnetic nanoparticle‑LFIA strips were used to assess 41 seropositive and 121 seronegative serum samples. The sensitivity was 100% (162/162) and the specificity was 100% (162/162). This method cannot only be used for the detection of TORCH IgM-specific antibodies, but it can potentially be developed for use in the diagnosis of other acute or recently identified autoimmune diseases.
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November-2015
Volume 36 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li X, Zhang Q, Hou P, Chen M, Hui W, Vermorken A, Luo Z, Li H, Li Q, Cui Y, Cui Y, et al: Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections. Int J Mol Med 36: 1319-1326, 2015
APA
Li, X., Zhang, Q., Hou, P., Chen, M., Hui, W., Vermorken, A. ... Cui, Y. (2015). Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections. International Journal of Molecular Medicine, 36, 1319-1326. https://doi.org/10.3892/ijmm.2015.2333
MLA
Li, X., Zhang, Q., Hou, P., Chen, M., Hui, W., Vermorken, A., Luo, Z., Li, H., Li, Q., Cui, Y."Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections". International Journal of Molecular Medicine 36.5 (2015): 1319-1326.
Chicago
Li, X., Zhang, Q., Hou, P., Chen, M., Hui, W., Vermorken, A., Luo, Z., Li, H., Li, Q., Cui, Y."Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections". International Journal of Molecular Medicine 36, no. 5 (2015): 1319-1326. https://doi.org/10.3892/ijmm.2015.2333