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MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia

  • Authors:
    • Yujiao Xing
    • Jianhua Fu
    • Haiping Yang
    • Li Yao
    • Lin Qiao
    • Yanna Du
    • Xindong Xue
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1253-1263
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    Published online on: September 17, 2015
       https://doi.org/10.3892/ijmm.2015.2347
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Abstract

In this study, we investigated the mechanisms through which microRNAs (miRNAs or miRs) regulate lung development after birth, as well as the role of miRNAs in the development of bronchopulmonary dysplasia (BPD). For this purpose, a total of 90 neonatal Wistar rats were randomly and equally assigned to either a model group or a control group. On postnatal days 3, 7 and 14, the lung tissues were collected for histological analysis to determine morphological changes. The expression levels of proliferating cell nuclear antigen (PCNA) and platelet endothelial cell adhesion molecule-1 (PECAM-1, also known as CD31) were measured by RT-qPCR and western blot analysis. A miRCURY™ LNA array was employed to screen for differentially expressed miRNAs, and the possible target genes of those miRNAs were predicted. Our results revealed that, compared with the control group, the following changes induced by hyperoxia were observed in the model group over time: a decrease in the number, but an increase in the size of the alveoli, and a decrease in the number of secondary septa formed. In the model group, from postnatal days 3-14, the mRNA and protein expression levels of PCNA and CD31 were significantly lower than those in the control group. The differentially expressed miRNAs between the 2 groups were identified on days 3, 7 and 14 after birth. Possible target genes were identified for 32 differentially expressed miRNAs. Taken together, these findings suggest that during the development of BPD, an alveolarization disorder with microvascular dysplasia co-exists with the differential expression of certain miRNAs during the different stages of alveolar development in a neonatal rat model of hyperoxia‑induced BPD. This indicates that miRNAs may participate in the occurrence and development of BPD.
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Copy and paste a formatted citation
Spandidos Publications style
Xing Y, Fu J, Yang H, Yao L, Qiao L, Du Y and Xue X: MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia. Int J Mol Med 36: 1253-1263, 2015.
APA
Xing, Y., Fu, J., Yang, H., Yao, L., Qiao, L., Du, Y., & Xue, X. (2015). MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia. International Journal of Molecular Medicine, 36, 1253-1263. https://doi.org/10.3892/ijmm.2015.2347
MLA
Xing, Y., Fu, J., Yang, H., Yao, L., Qiao, L., Du, Y., Xue, X."MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia". International Journal of Molecular Medicine 36.5 (2015): 1253-1263.
Chicago
Xing, Y., Fu, J., Yang, H., Yao, L., Qiao, L., Du, Y., Xue, X."MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia". International Journal of Molecular Medicine 36, no. 5 (2015): 1253-1263. https://doi.org/10.3892/ijmm.2015.2347
Copy and paste a formatted citation
x
Spandidos Publications style
Xing Y, Fu J, Yang H, Yao L, Qiao L, Du Y and Xue X: MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia. Int J Mol Med 36: 1253-1263, 2015.
APA
Xing, Y., Fu, J., Yang, H., Yao, L., Qiao, L., Du, Y., & Xue, X. (2015). MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia. International Journal of Molecular Medicine, 36, 1253-1263. https://doi.org/10.3892/ijmm.2015.2347
MLA
Xing, Y., Fu, J., Yang, H., Yao, L., Qiao, L., Du, Y., Xue, X."MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia". International Journal of Molecular Medicine 36.5 (2015): 1253-1263.
Chicago
Xing, Y., Fu, J., Yang, H., Yao, L., Qiao, L., Du, Y., Xue, X."MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia". International Journal of Molecular Medicine 36, no. 5 (2015): 1253-1263. https://doi.org/10.3892/ijmm.2015.2347
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