Open Access

Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation

  • Authors:
    • Yuechun Zhong
    • Liyi Zou
    • Zonggui Wang
    • Yaqiong Pan
    • Zhong Dai
    • Xinguang Liu
    • Liao Cui
    • Changqing Zuo
  • View Affiliations

  • Published online on: September 15, 2016     https://doi.org/10.3892/ijmm.2016.2738
  • Pages: 1411-1418
  • Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Many transcription factors and signaling molecules involved in the guidance of myogenic differentiation have been investigated in previous studies. However, the precise molecular mechanisms of myogenic differentiation remain largely unknown. In the present study, by performing a meta-analysis of C2C12 myogenic differentiation microarray data, we found that leucine-rich repeat-containing 75B (Lrrc75b), also known as AI646023, a molecule of unknown biological function, was downregulated during C2C12 myogenic differentiation. The knockdown of Lrrc75b using specific siRNA in C2C12 myoblasts markedly enhanced the expression of muscle-specific myogenin and increased myoblast fusion and the myotube diameter. By contrast, the adenovirus-mediated overexpression of Lrrc75b in C2C12 cells markedly inhibited myoblast differentiation accompanied by a decrease in myogenin expression. In addition, the phosphorylation of extracellular signal-regulated kinase 1/2 (Erk1/2) was suppressed in the cells in which Lrrc75b was silenced. Taken together, our results demonstrate that Lrrc75b is a novel suppressor of C2C12 myogenic differentiation by modulating myogenin and Erk1/2 signaling.
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November-2016
Volume 38 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhong Y, Zou L, Wang Z, Pan Y, Dai Z, Liu X, Cui L and Zuo C: Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation. Int J Mol Med 38: 1411-1418, 2016
APA
Zhong, Y., Zou, L., Wang, Z., Pan, Y., Dai, Z., Liu, X. ... Zuo, C. (2016). Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation. International Journal of Molecular Medicine, 38, 1411-1418. https://doi.org/10.3892/ijmm.2016.2738
MLA
Zhong, Y., Zou, L., Wang, Z., Pan, Y., Dai, Z., Liu, X., Cui, L., Zuo, C."Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation". International Journal of Molecular Medicine 38.5 (2016): 1411-1418.
Chicago
Zhong, Y., Zou, L., Wang, Z., Pan, Y., Dai, Z., Liu, X., Cui, L., Zuo, C."Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation". International Journal of Molecular Medicine 38, no. 5 (2016): 1411-1418. https://doi.org/10.3892/ijmm.2016.2738