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Article

Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages

  • Authors:
    • Young-Chang Cho
    • Ye Rang Kim
    • Ba Reum Kim
    • Tran The Bach
    • Sayeon Cho
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea, Vietnam Academy of Science and Technology (VAST), Hanoi 10307, Vietnam
  • Pages: 1596-1604
    |
    Published online on: September 21, 2016
       https://doi.org/10.3892/ijmm.2016.2746
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Abstract

Thunbergia alata (Acanthaceae) has been used traditionally to treat various inflammatory diseases such as fever, cough and diarrhea in East African countries including Uganda and Kenya. However, systemic studies elucidating the anti-inflammatory effects and precise mechanisms of action of T. alata have not been conducted, to the best of our knowledge. To address these concerns, we explored the anti-inflammatory effects of a methanol extract of T. alata (MTA) in macrophages. Non-cytotoxic concentrations of MTA (≤300 µg/ml) inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)‑stimulated RAW 264.7 macrophages by transcriptional regulation of inducible NO synthase in a dose-dependent manner. The expression of cyclooxygenase-2, the enzyme responsible for the production of prostaglandin E2, was unchanged by MTA at the mRNA and protein levels. MTA treatment inhibited interleukin (IL)-6 production and decreased the mRNA expression of pro‑inflammatory cytokines, including IL-6 and IL-1β. Tumor necrosis factor-α production and mRNA expression were not regulated by MTA treatment. The decreased production of inflammatory mediators by MTA was followed by the reduced phosphorylation of extracellular signal‑regulated kinase (ERK) and signal transducer and activator of transcription 3 (STAT3). MTA treatment had no effect on activity of other mitogen‑activated protein kinases (MAPKs), p38, c-Jun N-terminal kinase (JNK), and nuclear factor-κB (NF-κB). These results indicate that MTA selectively inhibits the excessive production of inflammatory mediators in LPS-stimulated murine macrophages by reducing the activity of ERK and STAT3, suggesting that MTA plays an important inhibitory role in the modulation of severe inflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Cho Y, Kim YR, Kim BR, Bach TT and Cho S: Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages. Int J Mol Med 38: 1596-1604, 2016.
APA
Cho, Y., Kim, Y.R., Kim, B.R., Bach, T.T., & Cho, S. (2016). Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages. International Journal of Molecular Medicine, 38, 1596-1604. https://doi.org/10.3892/ijmm.2016.2746
MLA
Cho, Y., Kim, Y. R., Kim, B. R., Bach, T. T., Cho, S."Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages". International Journal of Molecular Medicine 38.5 (2016): 1596-1604.
Chicago
Cho, Y., Kim, Y. R., Kim, B. R., Bach, T. T., Cho, S."Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages". International Journal of Molecular Medicine 38, no. 5 (2016): 1596-1604. https://doi.org/10.3892/ijmm.2016.2746
Copy and paste a formatted citation
x
Spandidos Publications style
Cho Y, Kim YR, Kim BR, Bach TT and Cho S: Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages. Int J Mol Med 38: 1596-1604, 2016.
APA
Cho, Y., Kim, Y.R., Kim, B.R., Bach, T.T., & Cho, S. (2016). Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages. International Journal of Molecular Medicine, 38, 1596-1604. https://doi.org/10.3892/ijmm.2016.2746
MLA
Cho, Y., Kim, Y. R., Kim, B. R., Bach, T. T., Cho, S."Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages". International Journal of Molecular Medicine 38.5 (2016): 1596-1604.
Chicago
Cho, Y., Kim, Y. R., Kim, B. R., Bach, T. T., Cho, S."Thunbergia alata inhibits inflammatory responses through the inactivation of ERK and STAT3 in macrophages". International Journal of Molecular Medicine 38, no. 5 (2016): 1596-1604. https://doi.org/10.3892/ijmm.2016.2746
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