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Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy

  • Authors:
    • Wen Zhou
    • Liu-Qing Zhou
    • Hong Shi
    • Yang-Ming Leng
    • Bo Liu
    • Su-Lin Zhang
    • Wei-Jia Kong
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1481-1489
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    Published online on: September 27, 2016
       https://doi.org/10.3892/ijmm.2016.2753
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Abstract

The medial vestibular nucleus (MVN) and the cerebellar flocculus have been known to be the key areas involved in vestibular compensation (VC) following unilateral labyrinthectomy (UL). In this study, we examined the role of gephyrin and glycine receptor (GlyR) in VC using Sprague-Dawley rats, in an aim to gain deeper insight into the mechanisms responsible for VC. The expression of the α1 and β subunits of GlyR and gephyrin was immunohistochemically localized in rat MVN and flocculi. The mRNA and protein expression of GlyR (α1 and β subunits) and gephyrin was quantitatively determined by RT-qPCR and western blot analysis at 8 h, and at 1, 3 and 7 days following UL. It was found that in the ipsilateral MVN, the mRNA and protein expression of the β subunit of GlyR was significantly increased in comparison to the sham-operated (P<0.01) rats, and in comparison to the contralateral side (P<0.01) at 8 h following UL. In the ipsilateral flocculi, GlyR β protein expression was significantly elevated (P<0.01 for all), as compared to the sham-operated rats at 8 h, and at 1 and 3 days and to the contralateral side 8 h, 1 and 3 days following UL. No significant differences were observed in the mRNA and protein expression of GlyR α1 and gephyrin in the MVN or flocculi between the two sides (ipsilateral and contralateral) in the UL group, and between the sham-operated group and the UL group at any time point. The findings of our study thus suggest that GlyR plays a major role in the recovery of the resting discharge of the deafferented MVN neurons in the central vestibular system.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou W, Zhou L, Shi H, Leng Y, Liu B, Zhang S and Kong W: Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy. Int J Mol Med 38: 1481-1489, 2016.
APA
Zhou, W., Zhou, L., Shi, H., Leng, Y., Liu, B., Zhang, S., & Kong, W. (2016). Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy. International Journal of Molecular Medicine, 38, 1481-1489. https://doi.org/10.3892/ijmm.2016.2753
MLA
Zhou, W., Zhou, L., Shi, H., Leng, Y., Liu, B., Zhang, S., Kong, W."Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy". International Journal of Molecular Medicine 38.5 (2016): 1481-1489.
Chicago
Zhou, W., Zhou, L., Shi, H., Leng, Y., Liu, B., Zhang, S., Kong, W."Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy". International Journal of Molecular Medicine 38, no. 5 (2016): 1481-1489. https://doi.org/10.3892/ijmm.2016.2753
Copy and paste a formatted citation
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Spandidos Publications style
Zhou W, Zhou L, Shi H, Leng Y, Liu B, Zhang S and Kong W: Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy. Int J Mol Med 38: 1481-1489, 2016.
APA
Zhou, W., Zhou, L., Shi, H., Leng, Y., Liu, B., Zhang, S., & Kong, W. (2016). Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy. International Journal of Molecular Medicine, 38, 1481-1489. https://doi.org/10.3892/ijmm.2016.2753
MLA
Zhou, W., Zhou, L., Shi, H., Leng, Y., Liu, B., Zhang, S., Kong, W."Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy". International Journal of Molecular Medicine 38.5 (2016): 1481-1489.
Chicago
Zhou, W., Zhou, L., Shi, H., Leng, Y., Liu, B., Zhang, S., Kong, W."Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy". International Journal of Molecular Medicine 38, no. 5 (2016): 1481-1489. https://doi.org/10.3892/ijmm.2016.2753
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