Open Access

Prediction of key genes and miRNAs responsible for loss of muscle force in patients during an acute exacerbation of chronic obstructive pulmonary disease

  • Authors:
    • Yanhong Duan
    • Min Zhou
    • Jian Xiao
    • Chaomin Wu
    • Lei Zhou
    • Feng Zhou
    • Chunling Du
    • Yuanlin Song
  • View Affiliations

  • Published online on: September 30, 2016     https://doi.org/10.3892/ijmm.2016.2761
  • Pages: 1450-1462
  • Copyright: © Duan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to identify genes and microRNAs (miRNAs or miRs) that were abnormally expressed in the vastus lateralis muscle of patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD). The gene expression profile of GSE10828 was downloaded from the Gene Expression Omnibus database, and this dataset was comprised of 4 samples from patients with AECOPD and 5 samples from patients with stable COPD. Differentially expressed genes (DEGs) were screened using the Limma package in R. A protein‑protein interaction (PPI) network of DEGs was built based on the STRING database. Module analysis of the PPI network was performed using the ClusterONE plugin and functional analysis of DEGs was conducted using DAVID. Additionally, key miRNAs were enriched using gene set enrichment analysis (GSEA) software and a miR-gene regulatory network was constructed using Cytoscape software. In total, 166 up- and 129 downregulated DEGs associated with muscle weakness in AECOPD were screened. Among them, NCL, GOT1, TMOD1, TSPO, SOD2, NCL and PA2G4 were observed in the modules consisting of upregulated or downregulated genes. The upregulated DEGs in modules (including KLF6 and XRCC5) were enriched in GO terms associated with immune system development, whereas the downregulated DEGs were enriched in GO terms associated with cell death and muscle contraction. Additionally, 39 key AECOPD‑related miRNAs were also predicted, including miR-1, miR-9 and miR-23a, miR-16 and miR-15a. In conclusion, DEGs (NCL, GOT1, SOD2, KLF6, XRCC5, TSPO and TMOD1) and miRNAs (such as miR-1, miR-9 and miR-23a) may be associated with the loss of muscle force in patients during an acute exacerbation of COPD which also may act as therapeutic targets in the treatment of AECOPD.
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November-2016
Volume 38 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Duan Y, Zhou M, Xiao J, Wu C, Zhou L, Zhou F, Du C and Song Y: Prediction of key genes and miRNAs responsible for loss of muscle force in patients during an acute exacerbation of chronic obstructive pulmonary disease. Int J Mol Med 38: 1450-1462, 2016
APA
Duan, Y., Zhou, M., Xiao, J., Wu, C., Zhou, L., Zhou, F. ... Song, Y. (2016). Prediction of key genes and miRNAs responsible for loss of muscle force in patients during an acute exacerbation of chronic obstructive pulmonary disease. International Journal of Molecular Medicine, 38, 1450-1462. https://doi.org/10.3892/ijmm.2016.2761
MLA
Duan, Y., Zhou, M., Xiao, J., Wu, C., Zhou, L., Zhou, F., Du, C., Song, Y."Prediction of key genes and miRNAs responsible for loss of muscle force in patients during an acute exacerbation of chronic obstructive pulmonary disease". International Journal of Molecular Medicine 38.5 (2016): 1450-1462.
Chicago
Duan, Y., Zhou, M., Xiao, J., Wu, C., Zhou, L., Zhou, F., Du, C., Song, Y."Prediction of key genes and miRNAs responsible for loss of muscle force in patients during an acute exacerbation of chronic obstructive pulmonary disease". International Journal of Molecular Medicine 38, no. 5 (2016): 1450-1462. https://doi.org/10.3892/ijmm.2016.2761