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The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells

  • Authors:
    • Hsiu-Chin Huang
    • Hsuan Lin
    • Min-Chuan Huang
  • View Affiliations / Copyright

    Affiliations: Renorigin Innovation Institute, Taipei 11560, Taiwan, R.O.C., Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei 100, Taiwan, R.O.C.
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 595-602
    |
    Published online on: February 9, 2017
       https://doi.org/10.3892/ijmm.2017.2884
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Abstract

Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immunomodulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this study, a set of five LfcinB-related peptides was examined. We found that LfB17‑34, an 18-mer LfcinB-derived peptide, increased melanogenesis in B16F10 melanoma cells without significantly affecting cell viability. LfB17‑34 increased in vitro tyrosinase activity and melanin content in a dose-dependent manner. The results of RT-qPCR and western blot analyses showed that LfB17‑34 increased the mRNA and protein expression of tyrosinase and tyrosinase-related protein 1 (Trp1). Moreover, LfB17‑34 inhibited the phosphorylation of MAPK/Erk, but not p38 and Akt, and constitutively active MEK was able to reverse the LfB17-34-enhanced pigmentation, melanin content, and tyrosinase activity, suggesting a role of Erk signaling in the process of LfB17‑34-mediated pigmentation. Taken together, these results suggest that LfB17‑34 induces melanogenesis in B16F10 cells primarily through increased tyrosinase expression and activity and that LfB17‑34 could be further developed for the treatment of hypopigmentation disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Huang H, Lin H and Huang M: The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells. Int J Mol Med 39: 595-602, 2017.
APA
Huang, H., Lin, H., & Huang, M. (2017). The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells. International Journal of Molecular Medicine, 39, 595-602. https://doi.org/10.3892/ijmm.2017.2884
MLA
Huang, H., Lin, H., Huang, M."The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells". International Journal of Molecular Medicine 39.3 (2017): 595-602.
Chicago
Huang, H., Lin, H., Huang, M."The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells". International Journal of Molecular Medicine 39, no. 3 (2017): 595-602. https://doi.org/10.3892/ijmm.2017.2884
Copy and paste a formatted citation
x
Spandidos Publications style
Huang H, Lin H and Huang M: The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells. Int J Mol Med 39: 595-602, 2017.
APA
Huang, H., Lin, H., & Huang, M. (2017). The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells. International Journal of Molecular Medicine, 39, 595-602. https://doi.org/10.3892/ijmm.2017.2884
MLA
Huang, H., Lin, H., Huang, M."The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells". International Journal of Molecular Medicine 39.3 (2017): 595-602.
Chicago
Huang, H., Lin, H., Huang, M."The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells". International Journal of Molecular Medicine 39, no. 3 (2017): 595-602. https://doi.org/10.3892/ijmm.2017.2884
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