Difficulty in obtaining the complete mRNA coding sequence at 5' region (5' end mRNA artifact): Causes, consequences in biology and medicine and possible solutions for obtaining the actual amino acid sequence of proteins (Review)

  • Authors:
    • Lorenza Vitale
    • Maria Caracausi
    • Raffaella Casadei
    • Maria Chiara Pelleri
    • Allison Piovesan
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  • Published online on: April 6, 2017     https://doi.org/10.3892/ijmm.2017.2942
  • Pages: 1063-1071
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Abstract

The known difficulty in obtaining the actual full length, complete sequence of a messenger RNA (mRNA) may lead to the erroneous determination of its coding sequence at the 5' region (5' end mRNA artifact), and consequently to the wrong assignment of the translation start codon, leading to the inaccurate prediction of the encoded polypeptide at its amino terminus. Among the known human genes whose study was affected by this artifact, we can include disco interacting protein 2 homolog A (DIP2A; KIAA0184), Down syndrome critical region 1 (DSCR1), SON DNA binding protein (SON), trefoil factor 3 (TFF3) and URB1 ribosome biogenesis 1 homolog (URB1; KIAA0539) on chromosome 21, as well as receptor for activated C kinase 1 (RACK1, also known as GNB2L1), glutaminyl‑tRNA synthetase (QARS) and tyrosyl-DNA phosphodiesterase 2 (TDP2) along with another 474 loci, including interleukin 16 (IL16). In this review, we discuss the causes of this issue, its quantitative incidence in biomedical research, the consequences in biology and medicine, and the possible solutions for obtaining the actual amino acid sequence of proteins in the post-genomics era.
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May-2017
Volume 39 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Vitale L, Caracausi M, Casadei R, Pelleri MC and Piovesan A: Difficulty in obtaining the complete mRNA coding sequence at 5' region (5' end mRNA artifact): Causes, consequences in biology and medicine and possible solutions for obtaining the actual amino acid sequence of proteins (Review). Int J Mol Med 39: 1063-1071, 2017
APA
Vitale, L., Caracausi, M., Casadei, R., Pelleri, M.C., & Piovesan, A. (2017). Difficulty in obtaining the complete mRNA coding sequence at 5' region (5' end mRNA artifact): Causes, consequences in biology and medicine and possible solutions for obtaining the actual amino acid sequence of proteins (Review). International Journal of Molecular Medicine, 39, 1063-1071. https://doi.org/10.3892/ijmm.2017.2942
MLA
Vitale, L., Caracausi, M., Casadei, R., Pelleri, M. C., Piovesan, A."Difficulty in obtaining the complete mRNA coding sequence at 5' region (5' end mRNA artifact): Causes, consequences in biology and medicine and possible solutions for obtaining the actual amino acid sequence of proteins (Review)". International Journal of Molecular Medicine 39.5 (2017): 1063-1071.
Chicago
Vitale, L., Caracausi, M., Casadei, R., Pelleri, M. C., Piovesan, A."Difficulty in obtaining the complete mRNA coding sequence at 5' region (5' end mRNA artifact): Causes, consequences in biology and medicine and possible solutions for obtaining the actual amino acid sequence of proteins (Review)". International Journal of Molecular Medicine 39, no. 5 (2017): 1063-1071. https://doi.org/10.3892/ijmm.2017.2942