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Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium

  • Authors:
    • Zhe Quan
    • Qiang-Li Wang
    • Pei Zhou
    • Guo-Dong Wang
    • Yu-Zhen Tan
    • Hai-Jie Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Histology and Embryology, Shanghai Medical School, Fudan University, Shanghai 200032, P.R. China, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China
    Copyright: © Quan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1347-1356
    |
    Published online on: April 11, 2017
       https://doi.org/10.3892/ijmm.2017.2950
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Abstract

The survival of transplanted stem cells in ischemic tissue is poor. In the present study, the effects of thymosin β4 (Tβ4) on the survival and angiogenesis of endothelial progenitor cells (EPCs) and improvement in cardiac functions after transplantation of Tβ4-treated EPCs in the infarcted myocardium were investigated. EPCs were isolated from bone marrow of adult male rats and incubated in Endothelial Basal Medium-2. Then the cells were treated with Tβ4 at different concentrations (0.05, 0.1 and 0.2 µM), and cells incubated with DMEM were set as controls. MTT assay, Transwell assay and tube formation in Matrigel were used to detect cell viability, migration and angiogenesis, respectively. For examining the protective effect of Tβ4 on EPCs, the cells were also incubated in the condition of hypoxia and serum deprivation. p-Akt expression was also examined using western blot analysis. Rat models of myocardial infarction (MI) were established by ligation of the anterior descending branch of the left coronary artery. At four weeks after intramyocardial injection of Tβ4-treated EPCs, the changes in cardiac functions, size of the scar tissue and density of microvessels were examined by echocardiography, Masson's trichrome staining, immunohistochemistry and fluorescence in situ hybridization (FISH) for the Y-chromosome. Tβ4 enhanced EPC viability, protected the cells from apoptosis in hypoxia and serum deprivation, and promoted the proliferation and migration of the cells and formation of capillary-like structures in the cells. Moreover, Tβ4 increased p-Akt expression in the cells. The cytoprotective and proangiogenic effects of Tβ4 were in a dose-dependent manner. Tβ4-treated EPCs improved cardiac function, enhanced the repair of the infarcted myocardium, and promoted angiogenesis after transplantation in the infarcted myocardium. In conclusion, pretreatment of EPCs with Tβ4 is a novel strategy for the repair of ischemic tissue after transplantation in MI.
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View References

1 

Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, Das SR, de Ferranti S, Després JP, Fullerton HJ, et al: Heart Disease and Stroke Statistics-2016 Update: A report from the American Heart Association. Circulation. 133:e38–e360. 2016. View Article : Google Scholar

2 

Diseases NCFC Report on cardiovascular disease in China 2014. Encyclopedia of China Publishing House; 2015

3 

White HD and Chew DP: Acute myocardial infarction. Lancet. 372:570–584. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Hochman JS, Lamas GA, Buller CE, Dzavik V, Reynolds HR, Abramsky SJ, Forman S, Ruzyllo W, Maggioni AP, White H, et al Occluded Artery Trial Investigators: Coronary intervention for persistent occlusion after myocardial infarction. N Engl J Med. 355:2395–2407. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Keeley EC, Boura JA and Grines CL: Primary angioplasty versus intravenous thrombolytic therapy for acute myocardial infarction: A quantitative review of 23 randomised trials. Lancet. 361:13–20. 2003. View Article : Google Scholar : PubMed/NCBI

6 

White H; Hirulog and Early Reperfusion or Occlusion (HERO)-2 Trial Investigators: Thrombin-specific anticoagulation with bivalirudin versus heparin in patients receiving fibrinolytic therapy for acute myocardial infarction: The HERO-2 randomised trial. Lancet. 358:1855–1863. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Freemantle N, Cleland J, Young P, Mason J and Harrison J: beta Blockade after myocardial infarction: systematic review and meta regression analysis. BMJ. 318:1730–1737. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Verma S and Strauss M: Angiotensin receptor blockers and myocardial infarction. BMJ. 329:1248–1249. 2004. View Article : Google Scholar : PubMed/NCBI

9 

McMurray J, Køber L, Robertson M, Dargie H, Colucci W, Lopez-Sendon J, Remme W, Sharpe DN and Ford I: Anti-arrhythmic effect of carvedilol after acute myocardial infarction: Results of the Carvedilol Post-Infarct Survival Control in Left Ventricular Dysfunction (CAPRICORN) trial. J Am Coll Cardiol. 45:525–530. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Yang X, Cohen MV and Downey JM: Mechanism of cardioprotection by early ischemic preconditioning. Cardiovasc Drugs Ther. 24:225–234. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Bergmann O, Bhardwaj RD, Bernard S, Zdunek S, Barnabé-Heider F, Walsh S, Zupicich J, Alkass K, Buchholz BA, Druid H, et al: Evidence for cardiomyocyte renewal in humans. Science. 324:98–102. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Werner N, Kosiol S, Schiegl T, Ahlers P, Walenta K, Link A, Böhm M and Nickenig G: Circulating endothelial progenitor cells and cardiovascular outcomes. N Engl J Med. 353:999–1007. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Yao EH, Fukuda N, Matsumoto T, Katakawa M, Yamamoto C, Han Y, Ueno T, Kobayashi N and Matsumoto K: Effects of the antioxidative β-blocker celiprolol on endothelial progenitor cells in hypertensive rats. Am J Hypertens. 21:1062–1068. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Sen S, Merchan J, Dean J, Ii M, Gavin M, Silver M, Tkebuchava T, Yoon YS, Rasko JE and Aikawa R: Autologous transplantation of endothelial progenitor cells genetically modified by adeno-associated viral vector delivering insulin-like growth factor-1 gene after myocardial infarction. Hum Gene Ther. 21:1327–1334. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Dubois C, Liu X, Claus P, Marsboom G, Pokreisz P, Vandenwijngaert S, Dépelteau H, Streb W, Chaothawee L, Maes F, et al: Differential effects of progenitor cell populations on left ventricular remodeling and myocardial neovascularization after myocardial infarction. J Am Coll Cardiol. 55:2232–2243. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Higuchi T, Anton M, Dumler K, Seidl S, Pelisek J, Saraste A, Welling A, Hofmann F, Oostendorp RA, Gansbacher B, et al: Combined reporter gene PET and iron oxide MRI for monitoring survival and localization of transplanted cells in the rat heart. J Nucl Med. 50:1088–1094. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Yao Y, Li Y, Ma G, Liu N, Ju S, Jin J, Chen Z, Shen C and Teng G: In vivo magnetic resonance imaging of injected endothelial progenitor cells after myocardial infarction in rats. Mol Imaging Biol. 13:303–313. 2011. View Article : Google Scholar

18 

Kupatt C, Horstkotte J, Vlastos GA, Pfosser A, Lebherz C, Semisch M, Thalgott M, Büttner K, Browarzyk C, Mages J, et al: Embryonic endothelial progenitor cells expressing a broad range of proangiogenic and remodeling factors enhance vascularization and tissue recovery in acute and chronic ischemia. FASEB J. 19:1576–1578. 2005.PubMed/NCBI

19 

Smart N, Risebro CA, Clark JE, Ehler E, Miquerol L, Rossdeutsch A, Marber MS and Riley PR: Thymosin β4 facilitates epicardial neovascularization of the injured adult heart. Ann NY Acad Sci. 1194:97–104. 2010. View Article : Google Scholar

20 

Sosne G, Xu L, Prach L, Mrock LK, Kleinman HK, Letterio JJ, Hazlett LD and Kurpakus-Wheater M: Thymosin beta 4 stimulates laminin-5 production independent of TGF-beta. Exp Cell Res. 293:175–183. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Yan L, Guan W, Hu Y, Xiaofan Y, Songshan X, Liya N and Yongzhe C: Recombinant thymosin β4 accelerates skin wound healing by regulating ICAM-1, MMP-2 and LN-5. J Tissue Engineering and Reconstructive Surgery. 163:142–145. 2008.

22 

Bock-Marquette I, Saxena A, White MD, Dimaio JM and Srivastava D: Thymosin β4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature. 432:466–472. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Smart N, Risebro CA, Melville AA, Moses K, Schwartz RJ, Chien KR and Riley PR: Thymosin β4 induces adult epicardial progenitor mobilization and neovascularization. Nature. 445:177–182. 2007. View Article : Google Scholar

24 

Sosne G, Siddiqi A and Kurpakus-Wheater M: Thymosin-β4 inhibits corneal epithelial cell apoptosis after ethanol exposure in vitro. Invest Ophthalmol Vis Sci. 45:1095–1100. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Qiu FY, Song XX, Zheng H, Zhao YB and Fu GS: Thymosin β4 induces endothelial progenitor cell migration via I3K/Akt/eNOS signal transduction pathway. J Cardiovasc Pharmacol. 53:209–214. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Burlacu A, Grigorescu G, Rosca AM, Preda MB and Simionescu M: Factors secreted by mesenchymal stem cells and endothelial progenitor cells have complementary effects on angiogenesis in vitro. Stem Cells Dev. 22:643–653. 2013. View Article : Google Scholar :

27 

Chang ZT, Hong L, Wang H, Lai HL, Li LF and Yin QL: Application of peripheral-blood-derived endothelial progenitor cell for treating ischemia-reperfusion injury and infarction: A preclinical study in rat models. J Cardiothorac Surg. 8:332013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Quan Z, Wang Q, Zhou P, Wang G, Tan Y and Wang H: Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium. Int J Mol Med 39: 1347-1356, 2017.
APA
Quan, Z., Wang, Q., Zhou, P., Wang, G., Tan, Y., & Wang, H. (2017). Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium. International Journal of Molecular Medicine, 39, 1347-1356. https://doi.org/10.3892/ijmm.2017.2950
MLA
Quan, Z., Wang, Q., Zhou, P., Wang, G., Tan, Y., Wang, H."Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium". International Journal of Molecular Medicine 39.6 (2017): 1347-1356.
Chicago
Quan, Z., Wang, Q., Zhou, P., Wang, G., Tan, Y., Wang, H."Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium". International Journal of Molecular Medicine 39, no. 6 (2017): 1347-1356. https://doi.org/10.3892/ijmm.2017.2950
Copy and paste a formatted citation
x
Spandidos Publications style
Quan Z, Wang Q, Zhou P, Wang G, Tan Y and Wang H: Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium. Int J Mol Med 39: 1347-1356, 2017.
APA
Quan, Z., Wang, Q., Zhou, P., Wang, G., Tan, Y., & Wang, H. (2017). Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium. International Journal of Molecular Medicine, 39, 1347-1356. https://doi.org/10.3892/ijmm.2017.2950
MLA
Quan, Z., Wang, Q., Zhou, P., Wang, G., Tan, Y., Wang, H."Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium". International Journal of Molecular Medicine 39.6 (2017): 1347-1356.
Chicago
Quan, Z., Wang, Q., Zhou, P., Wang, G., Tan, Y., Wang, H."Thymosin β4 promotes the survival and angiogenesis of transplanted endothelial progenitor cells in the infarcted myocardium". International Journal of Molecular Medicine 39, no. 6 (2017): 1347-1356. https://doi.org/10.3892/ijmm.2017.2950
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