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International Journal of Molecular Medicine
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Article

Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism

  • Authors:
    • Zhirong Zhang
    • Weiji Yang
    • Rongbiao Ying
    • Ying Shi
    • Huifang Jiang
    • Danli Cai
    • Jing Kuang
    • Ruhui Yang
    • Lingcong Wang
  • View Affiliations / Copyright

    Affiliations: Department of ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China, Postgraduates Department of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Surgical Oncology, Tumor Hospital of Taizhou, Wenling, Zhejiang 317500, P.R. China, Department of Hematology, Tongde Hospital of Zhejiang, Hangzhou, Zhejiang 310012, P.R. China, College of Medicine and Health, Lishui University, Lishui, Zhejiang 323000, P.R. China
  • Pages: 1580-1588
    |
    Published online on: April 26, 2017
       https://doi.org/10.3892/ijmm.2017.2969
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Abstract

The present study aimed to explore the influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism (APE) in rats. Our previous study found that CX3CL1/CX3CR1 was increased in APE. However, the effect of this signaling pathway on APE remains unclear. CX3CL1-shRNA adenovirus and CX3CL1-overexpression vector were constructed. Male Sprague-Dawley rats were randomly divided into 9 groups (n=10): normal group (group N), sham operation group (group Sham), sham operation + aspirin group (group ASP), model group (group M), model + ASP group (group M+A), model + shRNA group (group M+SH), sham operation + CX3CL1-overexpression vector group (group Sham+Cx3), model + ASP + shRNA group (group M+A+SH), and model + ASP + CX3CL1-overexpression vector group (group M+A+CX3). Arterial pressure detection, hematoxylin and eosin staining, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and laser confocal scanning microscopy were applied. Aspirin significantly decreased pulmonary artery pressure, improve pathological changes in the embolism, and decreased the expression of CX3CL1/CX3CR1 and CX3CL1/NF-κB. Moreover, the adenovirus-overexpression CX3CL1 vector aggravated the inflammatory changes in APE, which were improved by aspirin. However, the intervention of the adenovirus CX3CL1 vector reduced the change, while its combination with aspirin significantly improved the change. In conclusion, aspirin improved pathological changes in rats with APE via the CX3CL1/CX3CR1 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Yang W, Ying R, Shi Y, Jiang H, Cai D, Kuang J, Yang R and Wang L: Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism. Int J Mol Med 39: 1580-1588, 2017.
APA
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D. ... Wang, L. (2017). Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism. International Journal of Molecular Medicine, 39, 1580-1588. https://doi.org/10.3892/ijmm.2017.2969
MLA
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D., Kuang, J., Yang, R., Wang, L."Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism". International Journal of Molecular Medicine 39.6 (2017): 1580-1588.
Chicago
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D., Kuang, J., Yang, R., Wang, L."Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism". International Journal of Molecular Medicine 39, no. 6 (2017): 1580-1588. https://doi.org/10.3892/ijmm.2017.2969
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Yang W, Ying R, Shi Y, Jiang H, Cai D, Kuang J, Yang R and Wang L: Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism. Int J Mol Med 39: 1580-1588, 2017.
APA
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D. ... Wang, L. (2017). Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism. International Journal of Molecular Medicine, 39, 1580-1588. https://doi.org/10.3892/ijmm.2017.2969
MLA
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D., Kuang, J., Yang, R., Wang, L."Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism". International Journal of Molecular Medicine 39.6 (2017): 1580-1588.
Chicago
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D., Kuang, J., Yang, R., Wang, L."Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism". International Journal of Molecular Medicine 39, no. 6 (2017): 1580-1588. https://doi.org/10.3892/ijmm.2017.2969
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