A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer

  • Authors:
    • Guangjie He
    • Lu Yang
    • Xinlai Qian
    • Jing Li
    • Zhiqing Yuan
    • Changzheng Li
  • View Affiliations

  • Published online on: May 3, 2017     https://doi.org/10.3892/ijmm.2017.2974
  • Pages: 1571-1579
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Abstract

Cervical cancer is one of the most common gynecological malignancies worldwide. At present, the methods used for cervical cancer screening have many disadvantages. Matrix metalloproteinase-2 (MMP-2) is low or absent in normal cervical epithelial cells while overexpressed in cervical intraepithelial neoplasia and cervical cancer, which provides the possibility for the detection of cervical cancer based on MMP-2. The development of a strategy with high sensitivity and specificity for cervical cancer detection would be expected to improve the cure rate. Connecting a fluorophore [7-(diethylamino)-2-oxo-2H-chromene-3-succinimidyl ester] with a quencher {4-[4-(dimethylamino)phenylazo]benzoic acid N-succinimidyl ester} via an MMP-2 substrate peptide GPLGVRGKGG, a new coumarin-based fluorescence resonance energy transfer probe targeting MMP-2 was prepared to examine cervical cancer by cell imaging. The cervical cancer cell lines showed green fluorescence with intensities in the order of CaSki > SiHa > C33A > HeLa, which indicated that the cervical cancer cell lines possessed significantly differential expression levels of MMP-2. Quantitative (real‑time) PCR, RT-PCR, and western blot experiments were consistent with such results. The fluorescence detection limit of the probe for MMP-2 was estimated to be 0.05 ng/ml. Therefore, this MMP-2 probe potentially provides a sensitive and specific visual method for cervical cancer screening, diagnosis and prognostic judgment.
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June-2017
Volume 39 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
He G, Yang L, Qian X, Li J, Yuan Z and Li C: A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer. Int J Mol Med 39: 1571-1579, 2017
APA
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., & Li, C. (2017). A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer. International Journal of Molecular Medicine, 39, 1571-1579. https://doi.org/10.3892/ijmm.2017.2974
MLA
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., Li, C."A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer". International Journal of Molecular Medicine 39.6 (2017): 1571-1579.
Chicago
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., Li, C."A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer". International Journal of Molecular Medicine 39, no. 6 (2017): 1571-1579. https://doi.org/10.3892/ijmm.2017.2974