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Article

A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer

  • Authors:
    • Guangjie He
    • Lu Yang
    • Xinlai Qian
    • Jing Li
    • Zhiqing Yuan
    • Changzheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, School of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
  • Pages: 1571-1579
    |
    Published online on: May 3, 2017
       https://doi.org/10.3892/ijmm.2017.2974
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Abstract

Cervical cancer is one of the most common gynecological malignancies worldwide. At present, the methods used for cervical cancer screening have many disadvantages. Matrix metalloproteinase-2 (MMP-2) is low or absent in normal cervical epithelial cells while overexpressed in cervical intraepithelial neoplasia and cervical cancer, which provides the possibility for the detection of cervical cancer based on MMP-2. The development of a strategy with high sensitivity and specificity for cervical cancer detection would be expected to improve the cure rate. Connecting a fluorophore [7-(diethylamino)-2-oxo-2H-chromene-3-succinimidyl ester] with a quencher {4-[4-(dimethylamino)phenylazo]benzoic acid N-succinimidyl ester} via an MMP-2 substrate peptide GPLGVRGKGG, a new coumarin-based fluorescence resonance energy transfer probe targeting MMP-2 was prepared to examine cervical cancer by cell imaging. The cervical cancer cell lines showed green fluorescence with intensities in the order of CaSki > SiHa > C33A > HeLa, which indicated that the cervical cancer cell lines possessed significantly differential expression levels of MMP-2. Quantitative (real‑time) PCR, RT-PCR, and western blot experiments were consistent with such results. The fluorescence detection limit of the probe for MMP-2 was estimated to be 0.05 ng/ml. Therefore, this MMP-2 probe potentially provides a sensitive and specific visual method for cervical cancer screening, diagnosis and prognostic judgment.
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View References

1 

Torre LA, Siegel RL, Ward EM and Jemal A: Global cancer incidence and mortality rates and trends-an update. Cancer Epidemiol Biomarkers Prev. 25:16–27. 2016. View Article : Google Scholar

2 

Wang T, Wu MH, Wu YM and Zhang WY: A population-based study of invasive cervical cancer patients in Beijing: 1993–2008. Chin Med J (Engl). 128:3298–3304. 2015. View Article : Google Scholar

3 

Sankaranarayanan R, Qiao YL and Keita N: The next steps in cervical screening. Wom Health Lond. 11:201–212. 2015. View Article : Google Scholar

4 

Catarino R, Petignat P, Dongui G and Vassilakos P: Cervical cancer screening in developing countries at a crossroad: Emerging technologies and policy choices. World J Clin Oncol. 6:281–290. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Yi DK, Sun IC, Ryu JH, Koo H, Park CW, Youn IC, Choi K, Kwon IC, Kim K and Ahn CH: Matrix metalloproteinase sensitive gold nanorod for simultaneous bioimaging and photothermal therapy of cancer. Bioconjug Chem. 21:2173–2177. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Wang Y, Shen P, Li C, Wang Y and Liu Z: Upconversion fluorescence resonance energy transfer based biosensor for ultrasensitive detection of matrix metalloproteinase-2 in blood. Anal Chem. 84:1466–1473. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Wang H, Udukala DN, Samarakoon TN, Basel MT, Kalita M, Abayaweera G, Manawadu H, Malalasekera A, Robinson C, Villanueva D, et al: Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases. Photochem Photobiol Sci. 13:231–240. 2014. View Article : Google Scholar

8 

Zhu L, Xie J, Swierczewska M, Zhang F, Quan Q, Ma Y, Fang X, Kim K, Lee S and Chen X: Real-time video imaging of protease expression in vivo. Theranostics. 1:18–27. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Madsen DH and Bugge TH: The source of matrix-degrading enzymes in human cancer: Problems of research reproducibility and possible solutions. J Cell Biol. 209:195–198. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Theocharis AD, Skandalis SS, Gialeli C and Karamanos NK: Extracellular matrix structure. Adv Drug Deliv Rev. 97:4–27. 2016. View Article : Google Scholar

11 

Lombard C, Saulnier J and Wallach J: Assays of matrix metalloproteinases (MMPs) activities: A review. Biochimie. 87:265–272. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Tveitaras MK, Skogstrand T, Leh S, Helle F, Iversen BM, Chatziantoniou C, Reed RK and Hultstrom M: Matrix metalloproteinase-2 knockout and heterozygote mice are protected from hydronephrosis and kidney fibrosis after unilateral ureteral obstruction. PLoS One. 10:e01433902015. View Article : Google Scholar : PubMed/NCBI

13 

Xu C, Wang C, Cai QF, Zhang Q, Weng L, Liu GM, Su WJ and Cao MJ: Matrix metalloproteinase 2 (MMP-2) plays a critical role in the softening of common carp muscle during chilled storage by degradation of type I and V collagens. J Agric Food Chem. 63:10948–10956. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Yi GZ, Feng WY, Zhou Q, Liu YW and Qi ST: The impact of MMP-2 and its specific inhibitor TIMP-2 expression on the WHO grade and prognosis of gliomas in Chinese population: A Meta-Analysis. Mol Neurobiol. 54:22–30. 2017. View Article : Google Scholar :

15 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Ghosh A, Moirangthem A, Dalui R, Ghosh T, Bandyopadhyay A, Dasgupta A, Banerjee U, Jana N and Basu A: Expression of matrix metalloproteinase-2 and 9 in cervical intraepithelial neoplasia and cervical carcinoma among different age groups of premenopausal and postmenopausal women. J Cancer Res Clin Oncol. 140:1585–1593. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wang L, Wang Q, Li HL and Han LY: Expression of MiR200a, miR93, metastasis-related gene RECK and MMP2/MMP9 in human cervical carcinoma - relationship with prognosis. Asian Pac J Cancer Prev. 14:2113–2118. 2013. View Article : Google Scholar

18 

Schropfer A, Kammerer U, Kapp M, Dietl J, Feix S and Anacker J: Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines. BMC Cancer. 10:5532010. View Article : Google Scholar : PubMed/NCBI

19 

Xie B, Zhang Z, Wang H, Chen Z, Wang Y, Liang H, Yang G, Yang X and Zhang H: Genetic polymorphisms in MMP2, 3, 7, and 9 genes and the susceptibility and clinical outcome of cervical cancer in a Chinese Han population. Tumour Biol. 37:4883–4888. 2016. View Article : Google Scholar

20 

Dutra KL, Cordeiro MM, Vieira DS and Rivero ER: Immunohistochemical expression of matrix metalloproteinases in ameloblastomas and pericoronal follicles. J Oral Pathol Med. 45:586–590. 2016. View Article : Google Scholar

21 

Gunawardena I, Arendse M, Jameson MB, Plank LD and Gregor RT: Prognostic molecular markers in head and neck squamous cell carcinoma in a New Zealand population: Matrix metalloproteinase-2 and sialyl Lewis x antigen. ANZ J Surg. 85:843–848. 2015. View Article : Google Scholar

22 

Lee S, Ryu JH, Park K, Lee A, Lee SY, Youn IC, Ahn CH, Yoon SM, Myung SJ, Moon DH, et al: Polymeric nanoparticle- based activatable near-infrared nanosensor for protease determination in vivo. Nano Lett. 9:4412–4416. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Liang GX, Pan HC, Li Y, Jiang LP, Zhang JR and Zhu JJ: Near infrared sensing based on fluorescence resonance energy transfer between Mn:CdTe quantum dots and Au nanorods. Biosens Bioelectron. 24:3693–3697. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Bremer C, Bredow S, Mahmood U, Weissleder R and Tung CH: Optical imaging of matrix metalloproteinase-2 activity in tumors: Feasibility study in a mouse model. Radiology. 221:523–529. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Zajac A, Song D, Qian W and Zhukov T: Protein microarrays and quantum dot probes for early cancer detection. Colloids Surf B Biointerfaces. 58:309–314. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Li X, Deng D, Xue J, Qu L, Achilefu S and Gu Y: Quantum dots based molecular beacons for in vitro and in vivo detection of MMP-2 on tumor. Biosens Bioelectron. 61:512–518. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Schaferling M: The art of fluorescence imaging with chemical sensors. Angew Chem Int Ed Engl. 51:3532–3554. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Knight CG, Willenbrock F and Murphy G: A novel coumarin- labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEBS Lett. 296:263–266. 1992. View Article : Google Scholar : PubMed/NCBI

29 

Aggarwal M, Sharma R, Kumar P, Parida M and Tomar S: Kinetic characterization of trans-proteolytic activity of Chikungunya virus capsid protease and development of a FRET-based HTS assay. Sci Rep. 5:147532015. View Article : Google Scholar : PubMed/NCBI

30 

Zhang M, Yin BC, Wang XF and Ye BC: Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity. Chem Commun (Camb). 47:2399–2401. 2011. View Article : Google Scholar

31 

Myochin T, Hanaoka K, Komatsu T, Terai T and Nagano T: Design strategy for a near-infrared fluorescence probe for matrix metalloproteinase utilizing highly cell permeable boron dipyr- romethene. J Am Chem Soc. 134:13730–13737. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Kim J, Cote LJ, Kim F and Huang J: Visualizing graphene based sheets by fluorescence quenching microscopy. J Am Chem Soc. 132:260–267. 2010. View Article : Google Scholar

33 

Feng D, Zhang Y, Feng T, Shi W, Li X and Ma H: A graphene oxide-peptide fluorescence sensor tailor-made for simple and sensitive detection of matrix metalloproteinase 2. Chem Commun (Camb). 47:10680–10682. 2011. View Article : Google Scholar

34 

Ma Y, Luo W, Quinn PJ, Liu Z and Hider RC: Design, synthesis, physicochemical properties, and evaluation of novel iron chelators with fluorescent sensors. J Med Chem. 47:6349–6362. 2004. View Article : Google Scholar : PubMed/NCBI

35 

He G, Guo D, He C, Zhang X, Zhao X and Duan C: A color- tunable europium complex emitting three primary colors and white light. Angew Chem Int Ed Engl. 48:6132–6135. 2009. View Article : Google Scholar

36 

Albers AE, Okreglak VS and Changng CJ: A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide. J Am Chem Soc. 128:9640–9641. 2006. View Article : Google Scholar : PubMed/NCBI

37 

Zhu L, Zhang F, Ma Y, Liu G, Kim K, Fang X, Lee S and Chen X: In vivo optical imaging of membrane-type matrix metalloproteinase (MT-MMP) activity. Mol Pharm. 8:2331–2338. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Fields GB: Using fluorogenic peptide substrates to assay matrix metalloproteinases. Methods Mol Biol. 622:393–433. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Netzel-Arnett S, Mallya SK, Nagase H, Birkedal-Hansen H and Van Wart HE: Continuously recording fluorescent assays optimized for five human matrix metalloproteinases. Anal Biochem. 195:86–92. 1991. View Article : Google Scholar : PubMed/NCBI

40 

Song E, Cheng D, Song Y, Jiang M, Yu J and Wang Y: A graphene oxide-based FRET sensor for rapid and sensitive detection of matrix metalloproteinase 2 in human serum sample. Biosens Bioelectron. 47:445–450. 2013. View Article : Google Scholar : PubMed/NCBI

41 

Udukala DN, Wang H, Wendel SO, Malalasekera AP, Samarakoon TN, Yapa AS, Abayaweera G, Basel MT, Maynez P, Ortega R, et al: Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection. Beilstein J Nanotechnol. 7:364–373. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
He G, Yang L, Qian X, Li J, Yuan Z and Li C: A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer. Int J Mol Med 39: 1571-1579, 2017.
APA
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., & Li, C. (2017). A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer. International Journal of Molecular Medicine, 39, 1571-1579. https://doi.org/10.3892/ijmm.2017.2974
MLA
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., Li, C."A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer". International Journal of Molecular Medicine 39.6 (2017): 1571-1579.
Chicago
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., Li, C."A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer". International Journal of Molecular Medicine 39, no. 6 (2017): 1571-1579. https://doi.org/10.3892/ijmm.2017.2974
Copy and paste a formatted citation
x
Spandidos Publications style
He G, Yang L, Qian X, Li J, Yuan Z and Li C: A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer. Int J Mol Med 39: 1571-1579, 2017.
APA
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., & Li, C. (2017). A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer. International Journal of Molecular Medicine, 39, 1571-1579. https://doi.org/10.3892/ijmm.2017.2974
MLA
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., Li, C."A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer". International Journal of Molecular Medicine 39.6 (2017): 1571-1579.
Chicago
He, G., Yang, L., Qian, X., Li, J., Yuan, Z., Li, C."A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer". International Journal of Molecular Medicine 39, no. 6 (2017): 1571-1579. https://doi.org/10.3892/ijmm.2017.2974
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