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International Journal of Molecular Medicine
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June-2017 Volume 39 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer

  • Authors:
    • Yi-Fei Chen
    • Shu-Ying Wang
    • You-Hui Yang
    • Jiang Zheng
    • Ting Liu
    • Li Wang
  • View Affiliations / Copyright

    Affiliations: The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, P.R. China, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
  • Pages: 1564-1570
    |
    Published online on: May 8, 2017
       https://doi.org/10.3892/ijmm.2017.2978
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Abstract

Late diagnosis and lack of specific therapeutic targets contribute to the low survival rate of patients with epithelial ovarian cancer (EOC), the most lethal gynecologic malignancy. Therefore, the screening of diagnostic markers and the identification of therapeutic targets are urgently required. Heat shock factor 1 (HSF1) has been demonstrated to be overexpressed in certain malignancies and to be involved in tumor initiation, development, transformation and metastasis. It is believed that HSF1 is a promising candidate for antitumor therapy. However, its expression pattern and function in ovarian cancer are far from being fully elucidated. Therefore, we examined the HSF1 expression in human EOC tissues, and evaluated its carcinogenesis-promoting activity in a xenograft tumor model. Examination of HSF1 expression in human EOC tissues was performed by immunohistochemical assay using ovarian tissue blots. Specific short hairpin RNA (shRNA) against HSF1 was employed to knockdown HSF1 in SKOV3 cells. Cell proliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay; cell cycle distribution and apoptosis were determined by flow cytometric analysis. In normal ovarian tissues, HSF1 was barely detected, whereas, high expression of HSF1 was found in malignant EOC tissues, including serous, mucinous, endometrioid, and clear cell EOC tissues. Suppressed proliferative activity and intensified apoptosis were observed in HSF1-knockdown SKOV3 cells. In nude mouse xenografts, downregulation of HSF1 was found to cause reduced carinogenesis, indicating the antitumor effect induced by modulation of HSF1 against EOC. Our findings suggest that HSF1 may be considered as a potential candidate diagnostic marker of human EOC, and that modulation of HSF1 could be a promising therapeutic strategy against human EOC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Wang S, Yang Y, Zheng J, Liu T and Wang L: Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer. Int J Mol Med 39: 1564-1570, 2017.
APA
Chen, Y., Wang, S., Yang, Y., Zheng, J., Liu, T., & Wang, L. (2017). Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer. International Journal of Molecular Medicine, 39, 1564-1570. https://doi.org/10.3892/ijmm.2017.2978
MLA
Chen, Y., Wang, S., Yang, Y., Zheng, J., Liu, T., Wang, L."Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer". International Journal of Molecular Medicine 39.6 (2017): 1564-1570.
Chicago
Chen, Y., Wang, S., Yang, Y., Zheng, J., Liu, T., Wang, L."Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer". International Journal of Molecular Medicine 39, no. 6 (2017): 1564-1570. https://doi.org/10.3892/ijmm.2017.2978
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Wang S, Yang Y, Zheng J, Liu T and Wang L: Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer. Int J Mol Med 39: 1564-1570, 2017.
APA
Chen, Y., Wang, S., Yang, Y., Zheng, J., Liu, T., & Wang, L. (2017). Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer. International Journal of Molecular Medicine, 39, 1564-1570. https://doi.org/10.3892/ijmm.2017.2978
MLA
Chen, Y., Wang, S., Yang, Y., Zheng, J., Liu, T., Wang, L."Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer". International Journal of Molecular Medicine 39.6 (2017): 1564-1570.
Chicago
Chen, Y., Wang, S., Yang, Y., Zheng, J., Liu, T., Wang, L."Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer". International Journal of Molecular Medicine 39, no. 6 (2017): 1564-1570. https://doi.org/10.3892/ijmm.2017.2978
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