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HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes

  • Authors:
    • Xiaohan Jin
    • Zhongwei Xu
    • Jin Cao
    • Rui Yan
    • Ruicheng Xu
    • Ruiqiong Ran
    • Yongqiang Ma
    • Wei Cai
    • Rong Fan
    • Yan Zhang
    • Xin Zhou
    • Yuming Li
  • View Affiliations / Copyright

    Affiliations: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China, Central Laboratory, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China, Department of Basic Medicine, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1409-1420
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    Published online on: May 8, 2017
       https://doi.org/10.3892/ijmm.2017.2979
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Abstract

Heme oxygenase-1 (HO-1) is an inducible and cytoprotective enzyme that provides a defense against oxidant damage. The present study screened 137 HO-1/interacting proteins using a profound co-immunoprecipitation (Co-IP) coupled with proteomics, and profiled the global HO-1 interactome network, including oxidative phosphorylation, endoplasmic reticulum and transport vesicle functions. Among these molecules, we observed that a novel interactor, emopamil-binding protein (EBP), is closely related to the cholesterol metabolism process. This study demonstrated that cholesterol promotes excessive oxidative stress and alters the energy metabolism in cardiomyocytes, further triggering numerous cardiovascular diseases. We observed that cholesterol caused the overexpression of EBP and HO-1 by the activation of AKT and Nrf2/mTOR pathways. In addition, HO-1 and EBP performed a myocardial protective function. The overexpression of HO-1 alleviated the cholesterol-induced excessive oxidative stress status by inhibition of the carbohydrate metabolism. Notably, we also confirmed that the loss of partial HO-1 activity aggravated the oxidative damage and cardiac systolic function induced by a high-fat diet in HO-1 heterozygous (HO-1+/-) mice. These findings indicate that the HO-1/EBP interaction plays a protective role in alleviating the dysfunction of oxidative stress and cardiac systolic function induced by cholesterol stimulation.
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Copy and paste a formatted citation
Spandidos Publications style
Jin X, Xu Z, Cao J, Yan R, Xu R, Ran R, Ma Y, Cai W, Fan R, Zhang Y, Zhang Y, et al: HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39: 1409-1420, 2017.
APA
Jin, X., Xu, Z., Cao, J., Yan, R., Xu, R., Ran, R. ... Li, Y. (2017). HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. International Journal of Molecular Medicine, 39, 1409-1420. https://doi.org/10.3892/ijmm.2017.2979
MLA
Jin, X., Xu, Z., Cao, J., Yan, R., Xu, R., Ran, R., Ma, Y., Cai, W., Fan, R., Zhang, Y., Zhou, X., Li, Y."HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes". International Journal of Molecular Medicine 39.6 (2017): 1409-1420.
Chicago
Jin, X., Xu, Z., Cao, J., Yan, R., Xu, R., Ran, R., Ma, Y., Cai, W., Fan, R., Zhang, Y., Zhou, X., Li, Y."HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes". International Journal of Molecular Medicine 39, no. 6 (2017): 1409-1420. https://doi.org/10.3892/ijmm.2017.2979
Copy and paste a formatted citation
x
Spandidos Publications style
Jin X, Xu Z, Cao J, Yan R, Xu R, Ran R, Ma Y, Cai W, Fan R, Zhang Y, Zhang Y, et al: HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39: 1409-1420, 2017.
APA
Jin, X., Xu, Z., Cao, J., Yan, R., Xu, R., Ran, R. ... Li, Y. (2017). HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. International Journal of Molecular Medicine, 39, 1409-1420. https://doi.org/10.3892/ijmm.2017.2979
MLA
Jin, X., Xu, Z., Cao, J., Yan, R., Xu, R., Ran, R., Ma, Y., Cai, W., Fan, R., Zhang, Y., Zhou, X., Li, Y."HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes". International Journal of Molecular Medicine 39.6 (2017): 1409-1420.
Chicago
Jin, X., Xu, Z., Cao, J., Yan, R., Xu, R., Ran, R., Ma, Y., Cai, W., Fan, R., Zhang, Y., Zhou, X., Li, Y."HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes". International Journal of Molecular Medicine 39, no. 6 (2017): 1409-1420. https://doi.org/10.3892/ijmm.2017.2979
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