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Article Open Access

miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1

  • Authors:
    • Jing Zhu
    • Zhejun Chen
    • Jinxing Tian
    • Zehui Meng
    • Mingda Ju
    • Gencheng Wu
    • Zhanzhuang Tian
  • View Affiliations / Copyright

    Affiliations: Department of Integrative Medicine and Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Institute of Acupuncture Research, WHO Collaborating Centre for Traditional Medicine, Fudan Institutes of Integrative Medicine, Fudan University, Shanghai 200032, P.R. China , Department of Anatomy, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 90-100
    |
    Published online on: May 9, 2017
       https://doi.org/10.3892/ijmm.2017.2981
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Abstract

Exposure to trauma is a potential contributor to anxiety; however, the molecular mechanisms responsible for trauma-induced anxiety require further clarification. In this study, in an aim to explore these mechanisms, we observed the changes in the hypothalamic pituitary adrenal (HPA) axis using a radioimmunoassay and the changes in anxiety-like behavior using the open field test and elevated plus maze test in a rat model following intervention with NBI‑27914, a specific corticotropin‑releasing hormone receptor 1 (CRHR1) antagonist. CRHR1 was found to be involved in trauma‑induced anxiety. We then applied bioinformatic analysis to screen microRNAs (miRNAs or miRs) that target CRHR1, and miR‑34b was determined to negatively regulate CRHR1 mRNA in primary hypothalamic neurons. The overexpression of miR‑34b in the paraventricular nucleus (PVN) by a miRNA agomir using a drug delivery system decreased the hyperactivity of the HPA axis and anxiety‑like behavior. Overall, the involvement of the HPA axis in trauma‑induced anxiety was demonstrated, and trauma-induced anxiety was attenuated by decreasing the hyperactivity of the HPA axis via miR‑34b by targeting CRHR1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Chen Z, Tian J, Meng Z, Ju M, Wu G and Tian Z: miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1. Int J Mol Med 40: 90-100, 2017.
APA
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., & Tian, Z. (2017). miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1. International Journal of Molecular Medicine, 40, 90-100. https://doi.org/10.3892/ijmm.2017.2981
MLA
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., Tian, Z."miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1". International Journal of Molecular Medicine 40.1 (2017): 90-100.
Chicago
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., Tian, Z."miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1". International Journal of Molecular Medicine 40, no. 1 (2017): 90-100. https://doi.org/10.3892/ijmm.2017.2981
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Chen Z, Tian J, Meng Z, Ju M, Wu G and Tian Z: miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1. Int J Mol Med 40: 90-100, 2017.
APA
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., & Tian, Z. (2017). miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1. International Journal of Molecular Medicine, 40, 90-100. https://doi.org/10.3892/ijmm.2017.2981
MLA
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., Tian, Z."miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1". International Journal of Molecular Medicine 40.1 (2017): 90-100.
Chicago
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., Tian, Z."miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1". International Journal of Molecular Medicine 40, no. 1 (2017): 90-100. https://doi.org/10.3892/ijmm.2017.2981
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