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MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo

  • Authors:
    • Mingxin Liu
    • Guizhou Tao
    • Qifeng Liu
    • Kun Liu
    • Xinchun Yang
  • View Affiliations / Copyright

    Affiliations: Cardiovascular Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China, Department of Cardiology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 57-64
    |
    Published online on: May 18, 2017
       https://doi.org/10.3892/ijmm.2017.2995
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Abstract

Atherosclerosis is a chronic arterial disease and the leading cause of stroke and myocardial infarction. Micro­RNAs (miRNAs or miRs) have been reported to act as essential modulators during the progression of atherosclerosis. Although miR-let-7g has been demonstrated to contribute to maintaining endothelial function and vascular homeostasis, it is not known whether miR-let-7g exerts a therapeutic effect on experimental atherosclerosis. The aim of this study was to investigate the effects of miR-let-7g on atherosclerosis in vivo and in vitro and to explore its underlying mechanisms. Data from our study showed that exogenous lectin‑like oxidized low‑density lipoprotein receptor‑1 (LOX-1 or OLR1) overexpression resulted in the significant promotion of proliferation and migration of human aortic smooth muscle cells (ASMCs), whereas such changes induced by LOX-1 were obviously suppressed by transfection of miR‑let‑7g. We later confirmed that LOX-1 is a potential target of miR-let-7g, and miR-let-7g markedly inhibited LOX-1 expression in ASMCs by directly binding to the 3' untranslated region of LOX-1. Furthermore, in a hyperlipidemic apolipoprotein E knockout (ApoE-/-) mouse model, intravenous delivery of miR-let-7g mimics obviously attenuated high-fat diet-induced neointima formation and atherosclerotic lesions, accompanied by the significant downregulation of LOX-1, which was consistent with the effect of miR-let-7g on ASMCs. Taken together, our data revealed that miR-let-7g exhibits anti-atherosclerotic activity, at least partially by targeting the LOX-1 signaling pathway. This study suggests that miR-let-7g may be a therapeutic candidate for treating atherosclerosis, and provides novel insight into miRNA-based therapy for this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Liu M, Tao G, Liu Q, Liu K and Yang X: MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo. Int J Mol Med 40: 57-64, 2017.
APA
Liu, M., Tao, G., Liu, Q., Liu, K., & Yang, X. (2017). MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo. International Journal of Molecular Medicine, 40, 57-64. https://doi.org/10.3892/ijmm.2017.2995
MLA
Liu, M., Tao, G., Liu, Q., Liu, K., Yang, X."MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo". International Journal of Molecular Medicine 40.1 (2017): 57-64.
Chicago
Liu, M., Tao, G., Liu, Q., Liu, K., Yang, X."MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo". International Journal of Molecular Medicine 40, no. 1 (2017): 57-64. https://doi.org/10.3892/ijmm.2017.2995
Copy and paste a formatted citation
x
Spandidos Publications style
Liu M, Tao G, Liu Q, Liu K and Yang X: MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo. Int J Mol Med 40: 57-64, 2017.
APA
Liu, M., Tao, G., Liu, Q., Liu, K., & Yang, X. (2017). MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo. International Journal of Molecular Medicine, 40, 57-64. https://doi.org/10.3892/ijmm.2017.2995
MLA
Liu, M., Tao, G., Liu, Q., Liu, K., Yang, X."MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo". International Journal of Molecular Medicine 40.1 (2017): 57-64.
Chicago
Liu, M., Tao, G., Liu, Q., Liu, K., Yang, X."MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo". International Journal of Molecular Medicine 40, no. 1 (2017): 57-64. https://doi.org/10.3892/ijmm.2017.2995
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