Open Access

Protective effect of Salvia miltiorrhiza Bunge on 5-fluorouracil-induced oral mucositis

  • Authors:
    • Do Rim Kim
    • Jinsung Kim
    • Ja Young Oh
    • Ha Young Kim
    • Young Joo Kim
    • Mun Seog Chang
  • View Affiliations

  • Published online on: May 23, 2017     https://doi.org/10.3892/ijmm.2017.2999
  • Pages: 39-46
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral mucositis is a common side-effect caused by chemotherapy or radiotherapy occurring in the majority of cancer patients and is characterized by inflammation and ulcers in the oral mucosa. In the present study, we examined the protective effects of Salvia miltiorrhiza Bunge (SM) on oral mucositis induced by 5-fluorouracil (5-FU) in human pharyngeal cells and golden Syrian hamsters. We investigated the proliferation and antioxidant abilities of SM using MTT, 2-diphenyl-1-picrylhydrazyl (DPPH) and reactive oxygen species (ROS) assays in vitro. Additionally, TUNEL assay was performed, and the expression levels of nuclear factor-κB (NF-κB), caspase-3 and proinflammatory cytokines were assessed by immunoblotting. The results showed that SM increased the cell proliferation rate in human pharyngeal cells up to 128.97±9.7% compared with this rate in the untreated cells and exerted protective effects on mucosal injury caused by 5-FU treatment. In addition, all concentrations of SM increased DPPH scavenging ability and blocked ROS generation in the treated cells. Taken together, following SM treatment, expression of NF-κB and cleaved caspase-3 were significantly decreased followed by inhibition of cell death. These data suggest that SM could be used for the prevention and treatment of oral mucositis caused by cancer therapies.

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July 2017
Volume 40 Issue 1

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Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Kim, D., Kim, J., Oh, J., Kim, H., Kim, Y., & Chang, M. (2017). Protective effect of Salvia miltiorrhiza Bunge on 5-fluorouracil-induced oral mucositis. International Journal of Molecular Medicine, 40, 39-46. https://doi.org/10.3892/ijmm.2017.2999
MLA
Kim, D., Kim, J., Oh, J., Kim, H., Kim, Y., Chang, M."Protective effect of Salvia miltiorrhiza Bunge on 5-fluorouracil-induced oral mucositis". International Journal of Molecular Medicine 40.1 (2017): 39-46.
Chicago
Kim, D., Kim, J., Oh, J., Kim, H., Kim, Y., Chang, M."Protective effect of Salvia miltiorrhiza Bunge on 5-fluorouracil-induced oral mucositis". International Journal of Molecular Medicine 40, no. 1 (2017): 39-46. https://doi.org/10.3892/ijmm.2017.2999