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International Journal of Molecular Medicine
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HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line

  • Authors:
    • Xiaofeng Zhu
    • Shuichuan Huang
    • Lili Zeng
    • Jieyi Ma
    • Suhong Sun
    • Feng Zeng
    • Fanli Kong
    • Xiaoming Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Breast and Thyroid Surgery, The Affiliated Hospital, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China, Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Pathology, The Affiliated Hospital, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China, Laboratory of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510000, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 411-417
    |
    Published online on: June 14, 2017
       https://doi.org/10.3892/ijmm.2017.3027
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Abstract

Epithelial‑mesenchymal transition (EMT) is a key mechanism underlying metastatic breast cancer. Reactive oxygen species (ROS) play an important role in EMT. Heme oxygenase‑1 (HMOX‑1) can reduce oxidative stress. However, the effect of HMOX‑1 on the EMT process in breast cancer cells is unknown. We treated the MCF‑7 breast cancer cell line with the HMOX‑1 inducer hemin and observed that hemin induced HMOX‑1 expression and inhibited migration, invasion and ROS generation in transforming growth factor‑β (TGF‑β)‑treated MCF‑7 cells using quantitative RT‑qPCR, western blotting, wound‑healing and cell invasion assays as well as fluorescent probe DCFDA. Hemin inhibited TGF‑β‑induced EMT in the MCF‑7 cells, whereas HMOX‑1 siRNA attenuated the suppressive effect of hemin as determined by the expression and cellular distribution of selected EMT markers. In summary, our results revealed that hemin treatment increased HMOX‑1 expression and inhibited TGF‑β‑induced EMT in MCF‑7 cells.
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View References

1 

Libson S and Lippman M: A review of clinical aspects of breast cancer. Int Rev Psychiatry. 26:4–15. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Criscitiello C, André F, Thompson AM, De Laurentiis M, Esposito A, Gelao L, Fumagalli L, Locatelli M, Minchella I, Orsi F, et al: Biopsy confirmation of metastatic sites in breast cancer patients: Clinical impact and future perspectives. Breast Cancer Res. 16:2052014. View Article : Google Scholar : PubMed/NCBI

3 

De Craene B and Berx G: Regulatory networks defining EMT during cancer initiation and progression. Nat Rev Cancer. 13:97–110. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Sharma RR, Pollock K, Hubel A and McKenna D: Mesenchymal stem or stromal cells: A review of clinical applications and manufacturing practices. Transfusion. 54:1418–1437. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Kupcova Skalnikova H: Proteomic techniques for characterisation of mesenchymal stem cell secretome. Biochimie. 95:2196–2211. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Akhurst RJ and Hata A: Targeting the TGFβ signalling pathway in disease. Nat Rev Drug Discov. 11:790–811. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Li B, Wen G, Zhao Y, Tong J and Hei TK: The role of TGFBI in mesothelioma and breast cancer: Association with tumor suppression. BMC Cancer. 12:2392012. View Article : Google Scholar : PubMed/NCBI

8 

Brown DI and Griendling KK: Regulation of signal transduction by reactive oxygen species in the cardiovascular system. Circ Res. 116:531–549. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Krstić J, Trivanović D, Mojsilović S and Santibanez JF: Transforming growth factor-beta and oxidative stress interplay: Implications in tumorigenesis and cancer progression. Oxid Med Cell Longev. 2015:6545942015. View Article : Google Scholar

10 

Jozkowicz A, Was H and Dulak J: Heme oxygenase-1 in tumors: Is it a false friend? Antioxid Redox Signal. 9:2099–2117. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Gueron G, Giudice J, Valacco P, Paez A, Elguero B, Toscani M, Jaworski F, Leskow FC, Cotignola J, Marti M, et al: Heme-oxygenase-1 implications in cell morphology and the adhesive behavior of prostate cancer cells. Oncotarget. 5:4087–4102. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Cherrington JM, Strawn LM and Shawver LK: New paradigms for the treatment of cancer: The role of anti-angiogenesis agents. Adv Cancer Res. 79:1–38. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Chen HW, Chao CY, Lin LL, Lu CY, Liu KL, Lii CK and Li CC: Inhibition of matrix metalloproteinase-9 expression by docosahexaenoic acid mediated by heme oxygenase 1 in 12-O-tetradecanoylphorbol-13-acetate-induced MCF-7 human breast cancer cells. Arch Toxicol. 87:857–869. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Zavadil J, Cermak L, Soto-Nieves N and Böttinger EP: Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition. EMBO J. 23:1155–1165. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Vincent T, Neve EP, Johnson JR, Kukalev A, Rojo F, Albanell J, Pietras K, Virtanen I, Philipson L, Leopold PL, et al: A SNAIL1-SMAD3/4 transcriptional repressor complex promotes TGF-beta mediated epithelial-mesenchymal transition. Nat Cell Biol. 11:943–950. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Zhang KH, Tian HY, Gao X, Lei WW, Hu Y, Wang DM, Pan XC, Yu ML, Xu GJ, Zhao FK, et al: Ferritin heavy chain-mediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 69:5340–5348. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Lim SO, Gu JM, Kim MS, Kim HS, Park YN, Park CK, Cho JW, Park YM and Jung G: Epigenetic changes induced by reactive oxygen species in hepatocellular carcinoma: Methylation of the E-cadherin promoter. Gastroenterology. 135:2128–2140. 2140.e1–2140.e8. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Lochter A, Galosy S, Muschler J, Freedman N, Werb Z and Bissell MJ: Matrix metalloproteinase stromelysin-1 triggers a cascade of molecular alterations that leads to stable epithelial-to-mesenchymal conversion and a premalignant phenotype in mammary epithelial cells. J Cell Biol. 139:1861–1872. 1997. View Article : Google Scholar

19 

Baum B and Georgiou M: Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling. J Cell Biol. 192:907–917. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Herszterg S, Leibfried A, Bosveld F, Martin C and Bellaiche Y: Interplay between the dividing cell and its neighbors regulates adherens junction formation during cytokinesis in epithelial tissue. Dev Cell. 24:256–270. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Tomita K, van Bokhoven A, van Leenders GJ, Ruijter ET, Jansen CF, Bussemakers MJ and Schalken JA: Cadherin switching in human prostate cancer progression. Cancer Res. 60:3650–3654. 2000.PubMed/NCBI

22 

Sawada K, Mitra AK, Radjabi AR, Bhaskar V, Kistner EO, Tretiakova M, Jagadeeswaran S, Montag A, Becker A, Kenny HA, et al: Loss of E-cadherin promotes ovarian cancer metastasis via alpha 5-integrin, which is a therapeutic target. Cancer Res. 68:2329–2339. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Tang B, Peng ZH, Yu PW, Yu G and Qian F: Expression and significance of Cx43 and E-cadherin in gastric cancer and metastatic lymph nodes. Med Oncol. 28:502–508. 2011. View Article : Google Scholar

24 

Chao YL, Shepard CR and Wells A: Breast carcinoma cells re-express E-cadherin during mesenchymal to epithelial reverting transition. Mol Cancer. 9:1792010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu X, Huang S, Zeng L, Ma J, Sun S, Zeng F, Kong F and Cheng X: HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line. Int J Mol Med 40: 411-417, 2017.
APA
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F. ... Cheng, X. (2017). HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line. International Journal of Molecular Medicine, 40, 411-417. https://doi.org/10.3892/ijmm.2017.3027
MLA
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F., Kong, F., Cheng, X."HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line". International Journal of Molecular Medicine 40.2 (2017): 411-417.
Chicago
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F., Kong, F., Cheng, X."HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line". International Journal of Molecular Medicine 40, no. 2 (2017): 411-417. https://doi.org/10.3892/ijmm.2017.3027
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu X, Huang S, Zeng L, Ma J, Sun S, Zeng F, Kong F and Cheng X: HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line. Int J Mol Med 40: 411-417, 2017.
APA
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F. ... Cheng, X. (2017). HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line. International Journal of Molecular Medicine, 40, 411-417. https://doi.org/10.3892/ijmm.2017.3027
MLA
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F., Kong, F., Cheng, X."HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line". International Journal of Molecular Medicine 40.2 (2017): 411-417.
Chicago
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F., Kong, F., Cheng, X."HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line". International Journal of Molecular Medicine 40, no. 2 (2017): 411-417. https://doi.org/10.3892/ijmm.2017.3027
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