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JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts

  • Authors:
    • Qin Song
    • Yuxin Xie
    • Qiheng Gou
    • Xiaoqiang Guo
    • Qian Yao
    • Xiaojun Gou
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy and Bioengineering, Chengdu University, Chengdu, Sichuan 610106, P.R. China, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 465-473
    |
    Published online on: June 26, 2017
       https://doi.org/10.3892/ijmm.2017.3040
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Abstract

Periplaneta americana extracts (PAEs) play a crucial role in skin wound healing. However, their molecular effects and signaling pathways in regenerating tissues and cells are not clear. In this study, we refined the PAE from Periplaneta americana to investigate the mechanisms underlying skin wound healing. The human keratinocyte line HaCaT was selected and a mouse model of deep second-degree thermal burn was established for in vitro and in vivo studies, respectively. PAE treatment induced the proliferation and migration of HaCaT cells and wound healing in the burn model. Furthermore, the effects of PAE on wound healing were found to depend on the Janus-activated kinase/signal transducer and activator of transcription 3 (JAK/STAT3) pathway and Smad3 activities, according to western blot analysis and immunohistochemical (IHC) assays in vitro and in vivo. Pretreatment with a STAT3 inhibitor blocked the cell proliferation and migration induced by PAE. The results indicate the wound-healing function of PAE via enhanced JAK/STAT3 signaling and Smad3 activities. Our studies provide a theoretical basis underlying the role of PAE in cutaneous wound healing.
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1 

Luo TS, Gao MT, Ma FF, Liu GM and Zhang CG: Research advances in pharmacological action and clinical application of Periplaneta americana. Agric Sci Technol. 13:888–892. 2012.

2 

Chen XH, Ran XZ, Sun RS, Shi CM, Su Y, Guo CH and Cheng TM: Protective effect of an extract from Periplaneta americana on hematopoiesis in irradiated rats. Int J Radiat Biol. 85:607–613. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Yan MC, Yang H and Yang RM: Observation of the clinical effects of W11-a12. Yunnan Med. 3:138–140. 1987.

4 

Pan XL: The observation and nurse care with Kangfuxin local medicine - change in treating diabetes acromelic gangrene. J Nurse Train. 11:26–27. 1996.

5 

Wang HL and Liu J: Clinical observation of patients burned at III degree in small proportion of total surface area treated with Kangfuxin. J Binzhou Med Coll. 22:3681999.

6 

Lin Q, Cao D, Yang YQ, et al: Study on action of Kangfuxin solution on experimental gastric ulcer. Chin Trad Pat Med. 23:122–124. 2001.

7 

Du GM and Li WL: The clinical and laboratory studies about effects of Kangfuxin on superoxide dismutase and cell immune function of old persons. Yunnan J Chin Trad Med. 10:32–33. 1989.

8 

Man HX, Huang L, Na KG, et al: Research progress of chemical component and biological activity in medicinal Periplaneta americana. Anti-infect Pharmacol. 11:403–407. 2014.

9 

Wang ZY, Huang XH, Xie YK, Chen S and Wang S: Influence of Kangfuxin liquid on the wound healing of experimental animal burns and scalds. J Tradit Chin Med. 52:1316–1317. 2011.

10 

Shu CX, Cheng TM and Yan GM: Effects of whole body irradiation on several components of skin wound extracellular matrix and the repair-promoting action of W11-a12. Chin J Traumatol. 11:604–607. 2001.

11 

Shu CX, Ye BL, Cheng TM and Xiao JS: Effects of irradiation and W11-a12 on anion-selective channel of mouse peritoneal macrophage. Acta Acade Med Milita Tertiae. 23:290–292. 2001.

12 

Chen XH, Cheng TM and Ai GP: Effects of systemic irradiation and W11-a12 on neutrophils in wounds. Acta Acade Med Milita Tertiae. 23:287–289. 2001.

13 

Chen XH, Sun RS, Cheng TM, et al: Neutrophil apoptosis in wound of irradiated rats and effects of W11-a12 in accelerating wound healing. Chin J Clin Rehabil. 9:108–110. 2005.

14 

Amadeu TP, Seabra AB, de Oliveira MG and Monte-Alto-Costa A: Nitric oxide donor improves healing if applied on inflammatory and proliferative phase. J Surg Res. 149:84–93. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Li PN, Li H, Zhong LX, Sun Y, Yu LJ, Wu ML, Zhang LL, Kong QY, Wang SY and Lv DC: Molecular events underlying maggot extract promoted rat in vivo and human in vitro skin wound healing. Wound Repair Regen. 23:65–73. 2015. View Article : Google Scholar

16 

Pakyari M, Farrokhi A, Maharlooei MK and Ghahary A: Critical role of transforming growth factor beta in different phases of wound healing. Adv Wound Care (New Rochelle). 2:215–224. 2013. View Article : Google Scholar

17 

Ren X, Ge M, Qin X, Xu P, Zhu P, Dang Y, Gu J and Ye X: S100a8/NF-κB signal pathway is involved in the 800-nm diode laser-induced skin collagen remodeling. Lasers Med Sci. 31:673–678. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Shi Y, Shu B, Yang R, Xu Y, Xing B, Liu J, Chen L, Qi S, Liu X, Wang P, et al: Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately. Stem Cell Res Ther. 6:1202015. View Article : Google Scholar : PubMed/NCBI

19 

Tokumaru S, Sayama K, Yamasaki K, Shirakata Y, Hanakawa Y, Yahata Y, Dai X, Tohyama M, Yang L, Yoshimura A, et al: SOCS3/CIS3 negative regulation of STAT3 in HGF-induced keratinocyte migration. Biochem Biophys Res Commun. 327:100–105. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Yasukawa H, Ohishi M, Mori H, Murakami M, Chinen T, Aki D, Hanada T, Takeda K, Akira S, Hoshijima M, et al: IL-6 induces an anti-inflammatory response in the absence of SOCS3 in macrophages. Nat Immunol. 4:551–556. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Lejeune D, Dumoutier L, Constantinescu S, Kruijer W, Schuringa JJ and Renauld JC: Interleukin-22 (IL-22) activates the JAK/STAT, ERK, JNK, and p38 MAP kinase pathways in a rat hepatoma cell line. Pathways that are shared with and distinct from IL-10. J Biol Chem. 277:33676–33682. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Wolk K, Witte E, Witte K, Warszawska K and Sabat R: Biology of interleukin-22. Semin Immunopathol. 32:17–31. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Yu R, Ding Y, Zhu L, Qu Y, Zhang C, Liu L and Chen L: IL-22 mediates the oral mucosal wound healing via STAT3 in keratinocytes. Arch Oral Biol. 72:14–20. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Sano S, Itami S, Takeda K, Tarutani M, Yamaguchi Y, Miura H, Yoshikawa K, Akira S and Takeda J: Keratinocyte-specific ablation of Stat3 exhibits impaired skin remodeling, but does not affect skin morphogenesis. EMBO J. 18:4657–4668. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Penn JW, Grobbelaar AO and Rolfe KJ: The role of the TGF-β family in wound healing, burns and scarring: A review. Int J Burns Trauma. 2:18–28. 2012.

26 

Hong HJ, Jin SE, Park JS, Ahn WS and Kim CK: Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex. Biomaterials. 29:4831–4837. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Biernacka A, Dobaczewski M and Frangogiannis NG: TGF-β signaling in fibrosis. Growth Factors. 29:196–202. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Werner S, Krieg T and Smola H: Keratinocyte-fibroblast interactions in wound healing. J Invest Dermatol. 127:998–1008. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Tavares Pereira DS, Lima-Ribeiro MH, de Pontes-Filho NT, Carneiro-Leão AM and Correia MT: Development of animal model for studying deep second-degree thermal burns. J Biomed Biotechnol. 2012:4608412012. View Article : Google Scholar :

30 

Zhang J, La X, Fan L, Li P, Yu Y, Huang Y, Ding J and Xing Y: Immunosuppressive effects of mesenchymal stem cell transplantation in rat burn models. Int J Clin Exp Pathol. 8:5129–5136. 2015.PubMed/NCBI

31 

Diegelmann RF and Evans MC: Wound healing: An overview of acute, fibrotic and delayed healing. Front Biosci. 9:283–289. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Hata S, Okamura K, Hatta M, Ishikawa H and Yamazaki J: Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin. J Pharmacol Sci. 124:230–243. 2014. View Article : Google Scholar

33 

Yamaoka H, Sumiyoshi H, Higashi K, Nakao S, Minakawa K, Sumida K, Saito K, Ikoma N, Mabuchi T, Ozawa A, et al: A novel small compound accelerates dermal wound healing by modifying infiltration, proliferation and migration of distinct cellular components in mice. J Dermatol Sci. 74:204–213. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Levy DE and Darnell JE Jr: Stats: Transcriptional control and biological impact. Nat Rev Mol Cell Biol. 3:651–662. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Zhu BM, Ishida Y, Robinson GW, Pacher-Zavisin M, Yoshimura A, Murphy PM and Hennighausen L: SOCS3 negatively regulates the gp130-STAT3 pathway in mouse skin wound healing. J Invest Dermatol. 128:1821–1829. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Murray PJ: The JAK-STAT signaling pathway: Input and output integration. J Immunol. 178:2623–2629. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Tokumaru S, Sayama K, Shirakata Y, Komatsuzawa H, Ouhara K, Hanakawa Y, Yahata Y, Dai X, Tohyama M, Nagai H, et al: Induction of keratinocyte migration via transactivation of the epidermal growth factor receptor by the antimicrobial peptide LL-37. J Immunol. 175:4662–4668. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Avitabile S, Odorisio T, Madonna S, Eyerich S, Guerra L, Eyerich K, Zambruno G, Cavani A and Cianfarani F: Interleukin-22 promotes wound repair in diabetes by improving keratinocyte pro-healing functions. J Invest Dermatol. 135:2862–2870. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Nelson AM, Katseff AS, Ratliff TS and Garza LA: Interleukin 6 and STAT3 regulate p63 isoform expression in keratinocytes during regeneration. Exp Dermatol. 25:155–157. 2016. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Song Q, Xie Y, Gou Q, Guo X, Yao Q and Gou X: JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts. Int J Mol Med 40: 465-473, 2017.
APA
Song, Q., Xie, Y., Gou, Q., Guo, X., Yao, Q., & Gou, X. (2017). JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts. International Journal of Molecular Medicine, 40, 465-473. https://doi.org/10.3892/ijmm.2017.3040
MLA
Song, Q., Xie, Y., Gou, Q., Guo, X., Yao, Q., Gou, X."JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts". International Journal of Molecular Medicine 40.2 (2017): 465-473.
Chicago
Song, Q., Xie, Y., Gou, Q., Guo, X., Yao, Q., Gou, X."JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts". International Journal of Molecular Medicine 40, no. 2 (2017): 465-473. https://doi.org/10.3892/ijmm.2017.3040
Copy and paste a formatted citation
x
Spandidos Publications style
Song Q, Xie Y, Gou Q, Guo X, Yao Q and Gou X: JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts. Int J Mol Med 40: 465-473, 2017.
APA
Song, Q., Xie, Y., Gou, Q., Guo, X., Yao, Q., & Gou, X. (2017). JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts. International Journal of Molecular Medicine, 40, 465-473. https://doi.org/10.3892/ijmm.2017.3040
MLA
Song, Q., Xie, Y., Gou, Q., Guo, X., Yao, Q., Gou, X."JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts". International Journal of Molecular Medicine 40.2 (2017): 465-473.
Chicago
Song, Q., Xie, Y., Gou, Q., Guo, X., Yao, Q., Gou, X."JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts". International Journal of Molecular Medicine 40, no. 2 (2017): 465-473. https://doi.org/10.3892/ijmm.2017.3040
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