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Article

Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200

  • Authors:
    • Nan Ge
    • Chunxia Mao
    • Qingbo Yang
    • Bin Han
    • Yongjie Wang
    • Linhao Xu
    • Xiuzhi Yang
    • Wenjie Jiao
    • Chuan Li
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Hematology, Τhe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Operation Room, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
  • Pages: 2221-2229
    |
    Published online on: March 5, 2019
       https://doi.org/10.3892/ijmm.2019.4124
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Abstract

The present study evaluated the association between single nucleotide polymorphism (SNP) rs3746444 and the risk of non‑small cell lung carcinoma (NSCLC) in a Chinese population. Computational analyses and luciferase assays were performed to investigate the regulatory relationship between miR‑499a and CD200. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot assays were performed to examine the effect of rs3746444 on the expression of miR‑499a and CD200. The results demonstrated a significant difference in the smoking history of patients carrying malignant pulmonary nodules and those carrying benign pulmonary nodules. Furthermore, CD200 was demonstrated to be a direct target of miR‑499a, and a miR‑499a binding site was located in the 3'UTR of CD200. Notably, the levels of miR‑499a in malignant pulmonary nodules were higher compared with benign pulmonary nodules, while the levels of CD200 were higher in benign pulmonary nodules compared with malignant pulmonary nodules. In addition, the subjects carrying the AA genotype of SNP rs3746444 exhibited upregulated miR‑499a expression and reduced CD200 expression, compared with the subjects carrying AG and GG genotypes. These findings indicate that the SNP rs3746444 in miR‑499a could affect the prognosis of NSCLC patients by regulating the expression of CD200.
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Copy and paste a formatted citation
Spandidos Publications style
Ge N, Mao C, Yang Q, Han B, Wang Y, Xu L, Yang X, Jiao W and Li C: Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200. Int J Mol Med 43: 2221-2229, 2019.
APA
Ge, N., Mao, C., Yang, Q., Han, B., Wang, Y., Xu, L. ... Li, C. (2019). Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200. International Journal of Molecular Medicine, 43, 2221-2229. https://doi.org/10.3892/ijmm.2019.4124
MLA
Ge, N., Mao, C., Yang, Q., Han, B., Wang, Y., Xu, L., Yang, X., Jiao, W., Li, C."Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200". International Journal of Molecular Medicine 43.5 (2019): 2221-2229.
Chicago
Ge, N., Mao, C., Yang, Q., Han, B., Wang, Y., Xu, L., Yang, X., Jiao, W., Li, C."Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200". International Journal of Molecular Medicine 43, no. 5 (2019): 2221-2229. https://doi.org/10.3892/ijmm.2019.4124
Copy and paste a formatted citation
x
Spandidos Publications style
Ge N, Mao C, Yang Q, Han B, Wang Y, Xu L, Yang X, Jiao W and Li C: Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200. Int J Mol Med 43: 2221-2229, 2019.
APA
Ge, N., Mao, C., Yang, Q., Han, B., Wang, Y., Xu, L. ... Li, C. (2019). Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200. International Journal of Molecular Medicine, 43, 2221-2229. https://doi.org/10.3892/ijmm.2019.4124
MLA
Ge, N., Mao, C., Yang, Q., Han, B., Wang, Y., Xu, L., Yang, X., Jiao, W., Li, C."Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200". International Journal of Molecular Medicine 43.5 (2019): 2221-2229.
Chicago
Ge, N., Mao, C., Yang, Q., Han, B., Wang, Y., Xu, L., Yang, X., Jiao, W., Li, C."Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200". International Journal of Molecular Medicine 43, no. 5 (2019): 2221-2229. https://doi.org/10.3892/ijmm.2019.4124
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