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Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice

  • Authors:
    • Nan Miao
    • Yuanbo Zhan
    • Yingying Xu
    • Haoze Yuan
    • Chunlin Qin
    • Feng Lin
    • Xiaohua Xie
    • Sen Mu
    • Mengtong Yuan
    • Haibin Mu
    • Shouli Guo
    • Ying Li
    • Bin Zhang
  • View Affiliations / Copyright

    Affiliations: Institute of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Periodontology and Oral Mucosa, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, TX 75246, USA, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Miao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2103-2117
    |
    Published online on: March 6, 2019
       https://doi.org/10.3892/ijmm.2019.4126
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Abstract

Family with sequence similarity 20‑member C (FAM20C), a recently characterized Golgi kinase, performs numerous biological functions by phosphorylating more than 100 secreted proteins. However, the role of FAM20C in the salivary glands remains undefined. The present study demonstrated that FAM20C is mainly located in the cytoplasm of duct epithelial cells in the salivary glands. Fam20cf/f; Mmtv‑Cre mice were created in which Fam20c was inactivated in the salivary gland cells and observed that the number of ducts and the ductal cross‑sectional area increased significantly, while the number of acinar cells was reduced. The granular convoluted tubules (GCTs) exhibited an accumulation of aberrant secretory granules, along with a reduced expression and altered distribution patterns of β nerve growth factor, α‑amylase and bone morphogenetic protein (BMP) 4. This abnormality suggested that the GCT cells were immature and exhibited defects in developmental and secretory functions. In accordance with the morphological alterations and the reduced number of acinar cells, FAM20C deficiency in the salivary glands significantly decreased the salivary flow rate. The Na+, Cl– and K+ concentrations in the saliva were all significantly increased due to dysfunction of the ducts. Furthermore, Fam20c deficiency significantly increased BMP2 and BMP7 expression, decreased BMP4 expression, and attenuated the canonical and noncanonical BMP signaling pathways in the salivary glands. Collectively, the results of the present study demonstrate that FAM20C is a key regulator of acinar and duct structure and duct maturation and provide a novel avenue for investigating novel therapeutic targets for oral diseases including xerostomia.
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Copy and paste a formatted citation
Spandidos Publications style
Miao N, Zhan Y, Xu Y, Yuan H, Qin C, Lin F, Xie X, Mu S, Yuan M, Mu H, Mu H, et al: Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice. Int J Mol Med 43: 2103-2117, 2019.
APA
Miao, N., Zhan, Y., Xu, Y., Yuan, H., Qin, C., Lin, F. ... Zhang, B. (2019). Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice. International Journal of Molecular Medicine, 43, 2103-2117. https://doi.org/10.3892/ijmm.2019.4126
MLA
Miao, N., Zhan, Y., Xu, Y., Yuan, H., Qin, C., Lin, F., Xie, X., Mu, S., Yuan, M., Mu, H., Guo, S., Li, Y., Zhang, B."Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice". International Journal of Molecular Medicine 43.5 (2019): 2103-2117.
Chicago
Miao, N., Zhan, Y., Xu, Y., Yuan, H., Qin, C., Lin, F., Xie, X., Mu, S., Yuan, M., Mu, H., Guo, S., Li, Y., Zhang, B."Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice". International Journal of Molecular Medicine 43, no. 5 (2019): 2103-2117. https://doi.org/10.3892/ijmm.2019.4126
Copy and paste a formatted citation
x
Spandidos Publications style
Miao N, Zhan Y, Xu Y, Yuan H, Qin C, Lin F, Xie X, Mu S, Yuan M, Mu H, Mu H, et al: Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice. Int J Mol Med 43: 2103-2117, 2019.
APA
Miao, N., Zhan, Y., Xu, Y., Yuan, H., Qin, C., Lin, F. ... Zhang, B. (2019). Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice. International Journal of Molecular Medicine, 43, 2103-2117. https://doi.org/10.3892/ijmm.2019.4126
MLA
Miao, N., Zhan, Y., Xu, Y., Yuan, H., Qin, C., Lin, F., Xie, X., Mu, S., Yuan, M., Mu, H., Guo, S., Li, Y., Zhang, B."Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice". International Journal of Molecular Medicine 43.5 (2019): 2103-2117.
Chicago
Miao, N., Zhan, Y., Xu, Y., Yuan, H., Qin, C., Lin, F., Xie, X., Mu, S., Yuan, M., Mu, H., Guo, S., Li, Y., Zhang, B."Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice". International Journal of Molecular Medicine 43, no. 5 (2019): 2103-2117. https://doi.org/10.3892/ijmm.2019.4126
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