Peimine suppresses interleukin‑1β‑induced inflammation via MAPK downregulation in chondrocytes

Chen,Kun; Lv,Zheng‑Tao; Zhou,Chen‑He; Liang,Shuang; Huang,Wen; Wang,Zheng‑Gang; Zhu,Wen‑Tao; Wang,Yu‑Ting; Jing,Xing‑Zhi; Lin,Hui; Guo,Feng‑Jing; Cheng,Peng; Chen,An‑Min

doi:10.3892/ijmm.2019.4141

Peimine suppresses interleukin‑1β‑induced inflammation via MAPK downregulation in chondrocytes

Authors:
- Kun Chen
- Zheng‑Tao Lv
- Chen‑He Zhou
- Shuang Liang
- Wen Huang
- Zheng‑Gang Wang
- Wen‑Tao Zhu
- Yu‑Ting Wang
- Xing‑Zhi Jing
- Hui Lin
- Feng‑Jing Guo
- Peng Cheng
- An‑Min Chen
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Affiliations: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Pathology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, P.R. China, Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
Published online on: March 21, 2019 https://doi.org/10.3892/ijmm.2019.4141
Pages: 2241-2251

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Abstract

Osteoarthritis (OA) is the most common type of degenerative joint disease and secreted inflammatory molecules serve a pivotal role in it. Peimine has been reported to have anti‑inflammatory activity. In order to investigate the potential therapeutic role of Peimine in OA, mouse articular chondrocytes were treated with IL‑1β and different doses of Peimine in vitro. The data revealed that Peimine not only suppressed IL‑1β‑induced production of nitric oxide (NO) and prostaglandin E2, but also reduced the protein levels of inducible NO synthase (iNOS) and cyclooxygenase‑2 (COX‑2). In addition, Peimine inhibited the IL‑1β‑induced mRNA expression of matrix metalloproteinase (MMP)‑1, MMP‑3, MMP‑9, MMP‑13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)‑4 and ADAMTS‑5. Furthermore, Peimine inhibited IL‑1β‑induced activation of the mitogen‑activated protein kinase (MAPK) pathway. The protective effect of Peimine on IL‑1β‑treated chondrocytes was attenuated following activation of the MAPK pathway, as demonstrated by the increased expression levels of MMP‑3, MMP‑13, ADAMTS‑5, iNOS and COX‑2 compared with the Peimine group. The in vivo data suggested that Peimine limited the development of OA in the mouse model. In general, the data indicate that Peimine suppresses IL‑1β‑induced inflammation in mouse chondrocytes by inhibiting the MAPK pathway, suggesting a promising therapeutic role for Peimine in the treatment of OA.

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