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Article

Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells

  • Authors:
    • Phil‑Dong Moon
    • Na‑Ra Han
    • Jin Soo Lee
    • Hyung‑Min Kim
    • Hyun‑Ja Jeong
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea, Department of Food Science and Technology and Research Institute for Basic Science, Hoseo University, Asan, Chungnam 31499, Republic of Korea
  • Pages: 2252-2258
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    Published online on: March 22, 2019
       https://doi.org/10.3892/ijmm.2019.4144
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Abstract

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic disorders, including atopic dermatitis and asthma. Ursolic acid (UA) has various pharmacological properties, such as antioxidant, anti‑inflammatory and anticancer. However, the effect of UA on TSLP regulation has not been fully elucidated. The aim of the present study was to analyze how UA regulates the production of TSLP in the human mast cell line HMC‑1. Enzyme‑linked immunosorbent assay, quantitative polymerase chain reaction analysis, western blotting, caspase‑1 assay and fluorescent measurements of intracellular calcium levels were conducted to analyze the regulatory effects of UA. The results revealed that UA inhibited TSLP production and mRNA expression. In addition, UA reduced the activation of nuclear factor‑κB and degradation of IκBα. Caspase‑1 activity was increased by exposure to phorbol myristate acetate plus calcium ionophore, whereas it was reduced by UA. Finally, UA treatment prevented an increase in intracellular calcium levels. These results indicated that UA may be a useful agent for the treatment and/or prevention of atopic and inflammatory diseases, and its effects are likely mediated by TSLP downregulation.
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Copy and paste a formatted citation
Spandidos Publications style
Moon PD, Han NR, Lee JS, Kim HM and Jeong HJ: Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells. Int J Mol Med 43: 2252-2258, 2019.
APA
Moon, P., Han, N., Lee, J.S., Kim, H., & Jeong, H. (2019). Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells. International Journal of Molecular Medicine, 43, 2252-2258. https://doi.org/10.3892/ijmm.2019.4144
MLA
Moon, P., Han, N., Lee, J. S., Kim, H., Jeong, H."Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells". International Journal of Molecular Medicine 43.5 (2019): 2252-2258.
Chicago
Moon, P., Han, N., Lee, J. S., Kim, H., Jeong, H."Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells". International Journal of Molecular Medicine 43, no. 5 (2019): 2252-2258. https://doi.org/10.3892/ijmm.2019.4144
Copy and paste a formatted citation
x
Spandidos Publications style
Moon PD, Han NR, Lee JS, Kim HM and Jeong HJ: Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells. Int J Mol Med 43: 2252-2258, 2019.
APA
Moon, P., Han, N., Lee, J.S., Kim, H., & Jeong, H. (2019). Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells. International Journal of Molecular Medicine, 43, 2252-2258. https://doi.org/10.3892/ijmm.2019.4144
MLA
Moon, P., Han, N., Lee, J. S., Kim, H., Jeong, H."Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells". International Journal of Molecular Medicine 43.5 (2019): 2252-2258.
Chicago
Moon, P., Han, N., Lee, J. S., Kim, H., Jeong, H."Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells". International Journal of Molecular Medicine 43, no. 5 (2019): 2252-2258. https://doi.org/10.3892/ijmm.2019.4144
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