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Article Open Access

Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear

  • Authors:
    • Fumika Hotta
    • Hiroshi Eguchi
    • Haruyuki Nakayama‑Imaohji
    • Tomomi Kuwahara
    • Ayano Tada
    • Hirofumi Yagi
    • Yoshikazu Shimomura
    • Shunji Kusaka
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Kindai University Faculty of Medicine, Osakasayama, Osaka 589‑8511, Japan, Department of Microbiology, Faculty of Medicine, Kagawa University, Miki, Kagawa 761‑0793, Japan, Department of Ophthalmology, Fuchu Eye Center, Izumi, Osaka 594‑0076, Japan
    Copyright: © Hotta et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1367-1376
    |
    Published online on: July 17, 2020
       https://doi.org/10.3892/ijmm.2020.4678
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Abstract

The present study elucidated the pathogenesis of allergic symptoms (AS) related to contact lens (CL) wear by assaying CL care solutions in lens storage cases and tears from subjects with AS using molecular biology techniques. A total of 15 CL storage cases were collected from subjects with AS (n=9) and healthy, asymptomatic control CL wearers (n=6). Bacterial populations in CL care solutions and tears were assayed by culture and 16S rDNA sequencing. Histamine levels in tears were measured by high‑performance liquid chromatography. Western blot analysis was performed to identify the bacteria recognized by tear IgE from subjects with AS. No significant differences were found in the culture results between the subjects with AS and asymptomatic subjects. Histamine was detected in 2 subjects with AS. Meta‑16S rDNA sequencing identified a cluster of 4 subjects with AS that were distinguished from others by principal coordinate analysis. Detailed population analysis revealed that the abundance of Gram‑positive bacteria in the microbiomes of CL care solutions used by the subjects with AS were higher than those of asymptomatic subjects (42.24±9.47 vs. 16.85±22.76% abundance). Among these, Streptococcus was the dominant genus (12.1‑18.3% abundance). Tear microbiome analysis revealed that the abundance of Streptococcus in the subjects with AS was significantly higher than that in other subjects (19.02±5.50 vs. 3.08±3.35%, P<0.01). Western blot analysis demonstrated that the tear IgE in all subjects with AS reacted with Streptococcus (100%), but not with Staphylococcus. On the whole, these results provide novel insight into the pathogenesis of AS and identify Streptococcus as an important factor in AS associated with CL wear.
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1 

Skotnitsky CC, Naduvilath TJ, Sweeney DF and Sankaridurg PR: Two presentations of contact lens-induced papillary conjunctivitis (CLPC) in hydrogel lens wear: Local and general. Optom Vis Sci. 83:27–36. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Solomon A: Allergic manifestations of contact lens wearing. Curr Opin Allergy Clin Immunol. 16:492–497. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Tan ME, Demirci G, Pearce D, Jalbert I, Sankaridurg P and Willcox MD: Contact lens-induced papillary conjunctivitis is associated with increased albumin deposits on extended wear hydrogel lenses. Adv Exp Med Biol. 506:951–955. 2002. View Article : Google Scholar

4 

Tagliaferri A, Love TE and Szczotka-Flynn LB: Risk factors for contact lens-induced papillary conjunctivitis associated with silicone hydrogel contact lens wear. Eye Contact Lens. 40:117–122. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Shin H, Price K, Albert L, Dodick J, Park L and Dominguez-Bello MG: Changes in the eye microbiota associated with contact lens wearing. MBio. 7:e001982016. View Article : Google Scholar : PubMed/NCBI

6 

Amann RI, Ludwig W and Schleifer KH: Phylogenetic identification and in situ detection of indivisual microbial cells without cultivation. Microbiol Rev. 59:143–169. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Human Microbiome Project Consortium: Structure, function and diversity of the healthy human microbiome. Nature. 486:207–214. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Ahmed S, Macfarlane GT, Fite A, McBain AJ, Gilbert P and Macfarlane S: Mucosa-associated bacterial diversity in relation to human terminal ileum and colonic biopsy samples. Appl Environ Microbiol. 73:7435–7442. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Grice EA, Kong HH, Renaud G, Young AC; NISC Comparative Sequencing Program; Bouffard GG, Blakesley RW, Wolfsberg TG, Turner ML and Segre JA: A diversity profile of the human skin microbiota. Genome Res. 18:1043–1050. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Dong Q, Brulc JM, Iovieno A, Bates B, Garoutte A, Miller D, Revanna KV, Gao X, Antonopoulos DA, Slepak VZ and Shestopalov VI: Diversity of bacteria at healthy human conjunctiva. Invest Ophthalmol Vis Sci. 52:5408–5413. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Eguchi H, Hotta F, Kuwahara T, Imaohji H, Miyazaki C, Hirose M, Kusaka S, Fukuda M and Shimomura Y: Diagnostic approach to ocular infections using various techniques from conventional culture to next-generation sequencing analysis. Cornea. 36(Suppl 1): S46–S52. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Huang Y, Yang B and Li W: Defining the normal core micro-biome of conjunctival microbial communities. Clin Microbiol Infect. 22:643.e7–643.e12. 2016. View Article : Google Scholar

13 

Bakhtiar SM, LeBlanc JG, Salvucci E, Ali A, Martin R, Langella P, Chatel JM, Miyoshi A, Bermúdez-Humarán LG and Azevedo V: Implications of the human microbiome in inflammatory bowel diseases. FEMS Microbiol Lett. 342:10–17. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER and Gordon JI: An obesity-associated gut microbiome with increased capacity for energy harvest. Nature. 444:1027–1031. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Chiacchierini E, Restuccia D and Vinci G: Evaluation of two different extraction methods for chromatographic determination of bioactive amines in tomato products. Talanta. 69:548–555. 2006. View Article : Google Scholar

16 

Morita H, Kuwahara T, Ohshima K, Sasamoto H, Itoh K, Hattori M, Hayashi T and Takami H: An improved DNA isolation method for metagenomic analysis of the microbial flora of the human intestine. Microbes Environ. 22:214–222. 2007. View Article : Google Scholar

17 

Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M and Glöckner FO: Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 41:e12013. View Article : Google Scholar :

18 

Aronesty E: Comparison of sequencing utility programs. Open Bioinformatics J. 7:1–8. 2013. View Article : Google Scholar

19 

Navas-Molina JA, Peralta-Sánchez JM, González A, McMurdie PJ, Vázquez-Baeza Y, Xu Z, Ursell LK, Lauber C, Zhou H, Song SJ, et al: Advancing our understanding of the human microbiome using QIIME. Methods Enzymol. 531:371–444. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Edgar RC: Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 26:2460–2461. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Kanda Y: Investigation of the freely available easy-to-use soft-ware 'EZR' for medical statistics. Bone Marrow Transplant. 48:452–458. 2013. View Article : Google Scholar

22 

Dantam J, McCanna DJ, Subbaraman LN, Papinski D, Lakkis C, Mirza A, Berntsen DA, Morgan P, Nichols JJ and Jones LW: Microbial contamination of contact lens storage cases during daily wear use. Optom Vis Sci. 93:925–932. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Boost MV and Cho P: Microbial flora of tears of orthokeratology patients, and microbial contamination of contact lenses and contact lens accessories. Optom Vis Sci. 82:451–458. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Yung MS, Boost M, Cho P and Yap M: Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic Physiol Opt. 27:11–21. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Willcox MD, Power KN, Stapleton F, Leitch C, Harmis N and Sweeney DF: Potential sources of bacteria that are isolated from contact lenses during wear. Optom Vis Sci. 74:1030–1038. 1997. View Article : Google Scholar

26 

Wu YT, Zhu H, Harmis NY, Iskandar SY, Willcox M and Stapleton F: Profile and frequency of microbial contamination of contact lens cases. Optom Vis Sci. 87:E152–E158. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Willcox MD, Carnt N, Diec J, Naduvilath T, Evans V, Stapleton F, Iskandar S, Harmis N, de la Jara PL and Holden BA: Contact lens case contamination during daily wear of silicone hydrogels. Optom Vis Sci. 87:456–464. 2010.PubMed/NCBI

28 

McLaughlin-Borlace L, Stapleton F, Matheson M and Dart JK: Bacterial biofilm on contact lenses and lens storage cases in wearers with microbial keratitis. J Appl Microbiol. 84:827–838. 1998. View Article : Google Scholar : PubMed/NCBI

29 

Staley JT and Konopka A: Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats. Annu Rev Microbiol. 39:321–346. 1985. View Article : Google Scholar : PubMed/NCBI

30 

Eguchi H, Hotta F, Kuwahara T, Nakayama-Imaohji H, Kusaka S and Shimomura Y: Acute keratoconjunctivitis due to contamination of contact lens care solution with histamine-producing Raoultella species. A case report. Medicine (Baltimore). 96:e93102017. View Article : Google Scholar

31 

Nichols KK: Patient-reported symptoms in dry eye disease. Ocul Surf. 4:137–145. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Belmonte C: Pain, dryness, and itch sensations in eye surface disorders are defined by a balance between inflammation and sensory nerve injury. Cornea. 38(Suppl 1): S11–S24. 2019. View Article : Google Scholar : PubMed/NCBI

33 

Stapleton F, Stretton S, Sankaridurg PR, Chandoha H and Shovlin J: Hypersensitivity responses and contact lens wear. Cont Lens Anterior Eye. 26:57–69. 2003. View Article : Google Scholar

34 

Zhao Z, Fu H, Skotnitsky CC, Sankaridurg PR and Willcox MD: IgE antibody on worn highly oxygen-permeable silicone hydrogel contact lenses from patients with contact lens-induced papillary conjunctivitis (CLPC). Eye Contact Lens. 34:117–121. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Barishak Y, Zavaro A, Samra Z and Sompolinsky D: An immunological study of papillary conjunctivitis due to contact lenses. Curr Eye Res. 3:1161–1168. 1984. View Article : Google Scholar : PubMed/NCBI

36 

Bisgaard H, Hermansen MN, Buchvald F, Loland L, Halkjaer LB, Bønnelykke K, Brasholt M, Heltberg A, Vissing NH, Thorsen SV, et al: Childhood asthma after bacterial colonization of the airway in neonates. N Engl J Med. 357:1487–1495. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Jounio U, Juvonen R, Bloigu A, Silvennoinen-Kassinen S, Kaijalainen T, Kauma H, Peitso A, Saukkoriipi A, Vainio O, Harju T and Leinonen M: Pneumococcal carriage is more common in asthmatic than in non-asthmatic young men. Clin Respir J. 4:222–229. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Teo SM, Mok D, Pham K, Kusel M, Serralha M, Troy N, Holt BJ, Hales BJ, Walker ML, Hollams E, et al: The infant airway micro-biome in health and disease impacts later asthma development. Cell Host Microbe. 17:704–715. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Zhang Q, Cox M, Liang Z, Brinkmann F, Cardenas PA, Duff R, Bhavsar P, Cookson W, Moffatt M and Chung KF: Airway microbiota in severe asthma and relationship to asthma severity and phenotypes. PLoS One. 11:e01527242016. View Article : Google Scholar : PubMed/NCBI

40 

Kloepfer KM, Lee WM, Pappas TE, Kang TJ, Vrtis RF, Evans MD, Gangnon RE, Bochkov YA, Jackson DJ, Lemanske RF Jr, et al: Detection of pathogenic bacteria during rhinovirus infection is associated with increased respiratory symptoms and exacerbations of asthma. J Allergy Clin Immunol. 133:1301–1307. e1–e3. 2014. View Article : Google Scholar :

41 

Green BJ, Wiriyachaiporn S, Grainge C, Rogers GB, Kehagia V, Lau L, Carroll MP, Bruce KD and Howarth PH: Potentially pathogenic airway bacteria and neutrophilic inflammation in treatment resistant severe asthma. PLoS One. 9:e1006452014. View Article : Google Scholar : PubMed/NCBI

42 

Weisenseel P and Prinz JC: Incidental detection of S. pyogenes-DNA in psoriatic skin by PCR. Arch Dermatol Res. 296:573–576. 2005. View Article : Google Scholar : PubMed/NCBI

43 

Valdimarsson H, Thorleifsdottir RH, Sigurdardottir SL, Gudjonsson JE and Johnston A: Psoriasis-as an autoimmune disease caused by molecular mimicry. Trends Immunol. 30:494–501. 2009. View Article : Google Scholar : PubMed/NCBI

44 

Prinz JC: Psoriasis vulgaris-a sterile antibacterial skin reaction mediated by cross-reactive T cells? An immunological view of the pathophysiology of psoriasis. Clin Exp Dermatol. 26:326–332. 2001. View Article : Google Scholar : PubMed/NCBI

45 

Gudjonsson JE, Thorarinsson AM, Sigurgeirsson B, Kristinsson KG and Valdimarsson H: Streptococcal throat infections and exacerbation of chronic plaque psoriasis: A prospective study. Br J Dermatol. 149:530–534. 2003. View Article : Google Scholar : PubMed/NCBI

46 

Gray TB, Cursons RT, Sherwan JF and Rose PR: Acanthamoeba, bacterial, and fungal contamination of contact lens storage cases. Br J Ophthalmol. 79:601–605. 1995. View Article : Google Scholar : PubMed/NCBI

47 

Devonshire P, Munro FA, Abernethy C and Clark BJ: Microbial contamination of contact lens cases in the west of Scotland. Br J Ophthalmol. 77:41–45. 1993. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hotta F, Eguchi H, Nakayama‑Imaohji H, Kuwahara T, Tada A, Yagi H, Shimomura Y and Kusaka S: Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. Int J Mol Med 46: 1367-1376, 2020.
APA
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H. ... Kusaka, S. (2020). Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. International Journal of Molecular Medicine, 46, 1367-1376. https://doi.org/10.3892/ijmm.2020.4678
MLA
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H., Shimomura, Y., Kusaka, S."Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear". International Journal of Molecular Medicine 46.4 (2020): 1367-1376.
Chicago
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H., Shimomura, Y., Kusaka, S."Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear". International Journal of Molecular Medicine 46, no. 4 (2020): 1367-1376. https://doi.org/10.3892/ijmm.2020.4678
Copy and paste a formatted citation
x
Spandidos Publications style
Hotta F, Eguchi H, Nakayama‑Imaohji H, Kuwahara T, Tada A, Yagi H, Shimomura Y and Kusaka S: Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. Int J Mol Med 46: 1367-1376, 2020.
APA
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H. ... Kusaka, S. (2020). Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. International Journal of Molecular Medicine, 46, 1367-1376. https://doi.org/10.3892/ijmm.2020.4678
MLA
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H., Shimomura, Y., Kusaka, S."Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear". International Journal of Molecular Medicine 46.4 (2020): 1367-1376.
Chicago
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H., Shimomura, Y., Kusaka, S."Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear". International Journal of Molecular Medicine 46, no. 4 (2020): 1367-1376. https://doi.org/10.3892/ijmm.2020.4678
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