Open Access

Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear

  • Authors:
    • Fumika Hotta
    • Hiroshi Eguchi
    • Haruyuki Nakayama‑Imaohji
    • Tomomi Kuwahara
    • Ayano Tada
    • Hirofumi Yagi
    • Yoshikazu Shimomura
    • Shunji Kusaka
  • View Affiliations

  • Published online on: July 17, 2020     https://doi.org/10.3892/ijmm.2020.4678
  • Pages: 1367-1376
  • Copyright: © Hotta et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study elucidated the pathogenesis of allergic symptoms (AS) related to contact lens (CL) wear by assaying CL care solutions in lens storage cases and tears from subjects with AS using molecular biology techniques. A total of 15 CL storage cases were collected from subjects with AS (n=9) and healthy, asymptomatic control CL wearers (n=6). Bacterial populations in CL care solutions and tears were assayed by culture and 16S rDNA sequencing. Histamine levels in tears were measured by high‑performance liquid chromatography. Western blot analysis was performed to identify the bacteria recognized by tear IgE from subjects with AS. No significant differences were found in the culture results between the subjects with AS and asymptomatic subjects. Histamine was detected in 2 subjects with AS. Meta‑16S rDNA sequencing identified a cluster of 4 subjects with AS that were distinguished from others by principal coordinate analysis. Detailed population analysis revealed that the abundance of Gram‑positive bacteria in the microbiomes of CL care solutions used by the subjects with AS were higher than those of asymptomatic subjects (42.24±9.47 vs. 16.85±22.76% abundance). Among these, Streptococcus was the dominant genus (12.1‑18.3% abundance). Tear microbiome analysis revealed that the abundance of Streptococcus in the subjects with AS was significantly higher than that in other subjects (19.02±5.50 vs. 3.08±3.35%, P<0.01). Western blot analysis demonstrated that the tear IgE in all subjects with AS reacted with Streptococcus (100%), but not with Staphylococcus. On the whole, these results provide novel insight into the pathogenesis of AS and identify Streptococcus as an important factor in AS associated with CL wear.
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October-2020
Volume 46 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hotta F, Eguchi H, Nakayama‑Imaohji H, Kuwahara T, Tada A, Yagi H, Shimomura Y and Kusaka S: Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. Int J Mol Med 46: 1367-1376, 2020
APA
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H. ... Kusaka, S. (2020). Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. International Journal of Molecular Medicine, 46, 1367-1376. https://doi.org/10.3892/ijmm.2020.4678
MLA
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H., Shimomura, Y., Kusaka, S."Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear". International Journal of Molecular Medicine 46.4 (2020): 1367-1376.
Chicago
Hotta, F., Eguchi, H., Nakayama‑Imaohji, H., Kuwahara, T., Tada, A., Yagi, H., Shimomura, Y., Kusaka, S."Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear". International Journal of Molecular Medicine 46, no. 4 (2020): 1367-1376. https://doi.org/10.3892/ijmm.2020.4678