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Article Open Access

Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway

  • Authors:
    • Shumin Lv
    • Hongwen Cai
    • Yifei Xu
    • Jin Dai
    • Xiqing Rong
    • Lanzhi Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China, Department of Emergency Internal Medicine, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China
    Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1347-1358
    |
    Published online on: August 11, 2020
       https://doi.org/10.3892/ijmm.2020.4701
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Abstract

Thymosin‑β 4 (Tβ4) has been reported to exert a pro‑angogenic effect on endothelial cells. However, little is known on the role and underlying mechanisms of Tβ4 on critical limb ischemia (CLI). The present study aimed therefore to investigate the mechanisms and pro‑angiogenic effects of Tβ4 in CLI mice. Tβ4 overexpression lentiviral vector was first transfected into HUVEC and CLI mice model, and inhibitors of Notch pathway (DAPT) and NF‑κB pathway (BMS) were also applied to HUVEC and CLI mice. Subsequently, MTT, tube formation and wound healing assays were used to determine the cell viability, angiogenesis and migratory ablity of HUVEC, respectively. Western blotting, reverse transcription, quantitative PCR, immunofluorescence and immunohistochemistry were used to detect the expression of the angiogenesis‑related factors angiopoietin‑2 (Ang2), TEK receptor tyrosine kinase 2 (tie2), vascular endothelial growth factor A (VEGFA), CD31 and α‑smooth muscle actin (α‑SMA) and the Notch/NF‑κB pathways‑related factors NOTCH1 intracellular domain (N1ICD), Notch receptor 3 (Notch3), NF‑κB and p65 in HUVEC or CLI mice muscle tissues. The results demonstrated that Tβ4 not only enhanced the cell viability, angiogenesis and migratory ability of HUVEC but also promoted the expression of Ang2, tie2, VEGFA, N1ICD, Notch3, NF‑κB, and phosphorylated (p)‑p65 in HUVEC. In addition, Tβ4 promoted the expression of CD31, α‑SMA Ang2, tie2, VEGFA, N1ICD and p‑p65 in CLI mice muscle tissues. Treatment with DAPT and BMS had opposite effects of Tβ4, whereas Tβ4 reversed the effect of DAPT and BMS. The findings from the present study suggested that Tβ4 may promote angiogenesis in CLI mice via regulation of Notch/NF‑κB pathways.
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1 

Conte SM and Vale PR: Peripheral arterial disease. Heart Lung Circ. 27:427–432. 2018. View Article : Google Scholar

2 

Nehler MR, Duval S, Diao L, Annex BH, Hiatt WR, Rogers K, Zakharyan A and Hirsch AT: Epidemiology of peripheral arterial disease and critical limb ischemia in an insured national population. J Vasc Surg. 60:686–695 e682. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Dua A and Lee CJ: Epidemiology of peripheral arterial disease and critical limb ischemia. Tech Vasc Interv Radiol. 19:91–95. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Albrecht-Schgoer K, Barthelmes J, Schgoer W, Theurl M, Nardin I, Lener D, Gutmann C, Dünnhaupt S, Bernkop-Schnürch A and Kirchmair R: Nanoparticular delivery system for a secretoneurin derivative induces angiogenesis in a hind limb ischemia model. J Control Release. 250:1–8. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Constantinescu IM, Bolfa P, Constantinescu D, Mironiuc AI and Gherman CD: Treatment with sildenafil and donepezil improves angiogenesis in experimentally induced critical limb ischemia. Biomed Res Int. 2017:95323812017. View Article : Google Scholar : PubMed/NCBI

6 

Ishibashi T and Ryan SJ: Maturation of newly-formed subretinal vessels. EXS. 61:59–63. 1992.PubMed/NCBI

7 

Zhao C, Wang X, Zhao Y, Li Z, Lin S, Wei Y and Yang H: A novel xenograft model in zebrafish for high-resolution investigating dynamics of neovascularization in tumors. PLoS One. 6:e217682011. View Article : Google Scholar : PubMed/NCBI

8 

Sajib S, Zahra FT, Lionakis MS, German NA and Mikelis CM: mechanisms of angiogenesis in microbe-regulated inflammatory and neoplastic conditions. Angiogenesis. 21:1–14. 2018. View Article : Google Scholar

9 

Vasilopoulou E, Riley PR and Long DA: Thymosin-β4: A key modifier of renal disease. Expert Opin Biol Ther. 18:185–192. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Lv S, Cheng G, Zhou Y and Xu G: Thymosin β4 induces angiogenesis through Notch signaling in endothelial cells. Mol Cell Biochem. 381:283–290. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Sanders MC, Goldstein AL and Wang YL: Thymosin beta 4 (Fx peptide) is a potent regulator of actin polymerization in living cells. Proc Natl Acad Sci USA. 89:4678–4682. 1992. View Article : Google Scholar : PubMed/NCBI

12 

Kobayashi T, Okada F, Fujii N, Tomita N, Ito S, Tazawa H, Aoyama T, Choi SK, Shibata T, Fujita H and Hosokawa M: Thymosin-beta4 regulates motility and metastasis of malignant mouse fibrosarcoma cells. Am J Pathol. 160:869–882. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Renga G, Oikonomou V, Stincardini C, Pariano M, Borghi M, Costantini C, Bartoli A, Garaci E, Goldstein AL and Romani L: Thymosin β4 limits inflammation through autophagy. Expert Opin Biol Ther. 18(Suppl 1): S171–S175. 2018. View Article : Google Scholar

14 

Kleinman HK and Sosne G: Thymosin β4 promotes dermal healing. Vitam Horm. 102:251–275. 2016. View Article : Google Scholar

15 

Trenkwalder T, Deindl E, Bongiovanni D, Lee S, Schunkert H, Kupatt C and Hinkel R: Thymosin-β4-mediated therapeutic neovascularization: Role of the PI3K/AKT pathway. Expert Opin Biol Ther. 15(Suppl 1): S175–S185. 2015. View Article : Google Scholar

16 

Quan Z, Wang QL, Zhou P, Wang GD, Tan YZ and Wang HJ: Thymosin β4 promotes the survival and angiogenesis of trans-planted endothelial progenitor cells in the infarcted myocardium. Int J Mol Med. 39:1347–1356. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Zhao Y, Song J, Bi X, Gao J, Shen Z, Zhu J and Fu G: Thymosin β4 promotes endothelial progenitor cell angiogenesis via a vascular endothelial growth factor-dependent mechanism. Mol Med Rep. 18:2314–2320. 2018.PubMed/NCBI

18 

Zhao J, Liang Y, Song F, Xu S, Nian L, Zhou X and Wang S: TSG attenuates LPC-induced endothelial cells inflammatory damage through notch signaling inhibition. IUBMB Life. 68:37–50. 2016. View Article : Google Scholar

19 

MacMaster JF, Dambach DM, Lee DB, Berry KK, Qiu Y, Zusi FC and Burke JR: An inhibitor of IkappaB kinase, BMS-345541, blocks endothelial cell adhesion molecule expression and reduces the severity of dextran sulfate sodium-induced colitis in mice. Inflamm Res. 52:508–511. 2003. View Article : Google Scholar

20 

Grimaldo S, Tian F and Li LY: Sensitization of endothelial cells to VEGI-induced apoptosis by inhibiting the NF-kappaB pathway. Apoptosis. 14:788–795. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Burke JR, Pattoli MA, Gregor KR, Brassil PJ, Macmaster JF, McIntyre KW, Yang X, Iotzova VS, Clarke W, Strnad J, et al: BMS-345541 is a highly selective inhibitor of I kappa B kinase that binds at an allosteric site of the enzyme and blocks NF-kappa B-dependent transcription in mice. J Biol Chem. 278:1450–1456. 2003. View Article : Google Scholar

22 

Peng X, Zhou J, Li B, Zhang T, Zuo Y and Gu X: Notch1 and PI3K/Akt signaling blockers DAPT and LY294002 coordinately inhibit metastasis of gastric cancer through mutual enhancement. Cancer Chemother Pharmacol. 85:309–320. 2020. View Article : Google Scholar

23 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Farber A and Eberhardt RT: The current state of critical limb ischemia: A systematic review. JAMA Surg. 151:1070–1077. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Falluji N and Mukherjee D: Critical and acute limb ischemia: An overview. Angiology. 65:137–146. 2014. View Article : Google Scholar

26 

Liew A, Bhattacharya V, Shaw J and Stansby G: Cell therapy for critical limb ischemia: A meta-analysis of randomized controlled trials. Angiology. 67:444–455. 2016. View Article : Google Scholar

27 

Sosne G, Qiu P, Goldstein AL and Wheater M: Biological activities of thymosin beta4 defined by active sites in short peptide sequences. FASEB J. 24:2144–2151. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Yang WS, Kang S, Sung J and Kleinman HK: Thymosin β4: Potential to treat epidermolysis bullosa and other severe dermal injuries. Eur J Dermatol. 29:459–467. 2019. View Article : Google Scholar : PubMed/NCBI

29 

Zhang Z, Liu S and Huang S: Effects of thymosin β4 on neuronal apoptosis in a rat model of cerebral ischemiareperfusion injury. Mol Med Rep. 20:4186–4192. 2019.PubMed/NCBI

30 

Bjørklund G, Dadar M, Aaseth J and Chirumbolo S: Thymosin β4: A multi-faceted tissue repair stimulating protein in heart injury. Curr Med Chem. July 31–2019.Epub ahead of print. View Article : Google Scholar

31 

Zhang Y, Kontos CD, Annex BH and Popel AS: Angiopoietin-tie signaling pathway in endothelial cells: A computational model. iScience. 20:497–511. 2019. View Article : Google Scholar : PubMed/NCBI

32 

Saharinen P, Eklund L and Alitalo K: Therapeutic targeting of the angiopoietin-TIE pathway. Nat Rev Drug Discov. 16:635–661. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Thurston G and Daly C: The complex role of angiopoietin-2 in the angiopoietin-tie signaling pathway. Cold Spring Harb Perspect Med. 2:a0065502012. View Article : Google Scholar : PubMed/NCBI

34 

Perez-Moral N, Needs PW, moyle CWA and Kroon PA: Hydrophobic interactions drive binding between vascular endothelial growth factor-A (VEGFA) and polyphenolic inhibitors. Molecules. 24:27852019. View Article : Google Scholar :

35 

Bhisitkul RB: Vascular endothelial growth factor biology: Clinical implications for ocular treatments. Br J Ophthalmol. 90:1542–1547. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Shukla K, Sonowal H, Saxena A and Ramana KV: Didymin by suppressing NF-κB activation prevents VEGF-induced angiogenesis in vitro and in vivo. Vascul Pharmacol. 115:18–25. 2019. View Article : Google Scholar : PubMed/NCBI

37 

Huang DY, Dai ZR, Li WM, Wang RG and Yang SM: Inhibition of EGF expression and NF-κB activity by treatment with quercetin leads to suppression of angiogenesis in nasopharyngeal carcinoma. Saudi J Biol Sci. 25:826–831. 2018. View Article : Google Scholar : PubMed/NCBI

38 

Si W, Xie W, Deng W, Xiao Y, Karnik SS, Xu C, Chen Q and Wang QK: Angiotensin II increases angiogenesis by NF-κB-mediated transcriptional activation of angiogenic factor AGGF1. FASEB J. 32:5051–5062. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Li L, Tang P, Zhou Z, Wang Q, Xu T, Zhao S, Huang Y, Kong F, Liu W, Cheng L, et al: GIT1 regulates angiogenic factor secretion in bone marrow mesenchymal stem cells via NF-κB/notch signalling to promote angiogenesis. Cell Prolif. 52:e126892019. View Article : Google Scholar

40 

Xu D, Xia N, Hou K, Li F, Chen S, Hu Y, Fang W and Li Y: Clematichinenoside facilitates recovery of neurological and motor function in rats after cerebral ischemic injury through inhibiting notch/NF-κB pathway. J Stroke Cerebrovasc Dis. 28:1042882019. View Article : Google Scholar

41 

Saito T and Tanaka S: Molecular mechanisms underlying osteoarthritis development: Notch and NF-κB. Arthritis Res Ther. 19:942017. View Article : Google Scholar

42 

Chen Y, Zhao B, Zhu Y, Zhao H and Ma C: HIF-1-VEGF-notch mediates angiogenesis in temporomandibular joint osteoarthritis. Am J Transl Res. 11:2969–2982. 2019.PubMed/NCBI

43 

Luo Z, Shang X, Zhang H, Wang G, Massey PA, Barton SR, Kevil CG and Dong Y: Notch signaling in osteogenesis, osteoclastogenesis, and angiogenesis. Am J Pathol. 189:1495–1500. 2019. View Article : Google Scholar : PubMed/NCBI

44 

Huang F, Yao Y, Wu J, Liu Q, zhang J, Pu X, zhang Q and Xia L: Curcumin inhibits gastric cancer-derived mesenchymal stem cells mediated angiogenesis by regulating NF-κB/VEGF signaling. Am J Transl Res. 9:5538–5547. 2017.

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Copy and paste a formatted citation
Spandidos Publications style
Lv S, Cai H, Xu Y, Dai J, Rong X and Zheng L: Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway. Int J Mol Med 46: 1347-1358, 2020.
APA
Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X., & Zheng, L. (2020). Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway. International Journal of Molecular Medicine, 46, 1347-1358. https://doi.org/10.3892/ijmm.2020.4701
MLA
Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X., Zheng, L."Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway". International Journal of Molecular Medicine 46.4 (2020): 1347-1358.
Chicago
Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X., Zheng, L."Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway". International Journal of Molecular Medicine 46, no. 4 (2020): 1347-1358. https://doi.org/10.3892/ijmm.2020.4701
Copy and paste a formatted citation
x
Spandidos Publications style
Lv S, Cai H, Xu Y, Dai J, Rong X and Zheng L: Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway. Int J Mol Med 46: 1347-1358, 2020.
APA
Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X., & Zheng, L. (2020). Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway. International Journal of Molecular Medicine, 46, 1347-1358. https://doi.org/10.3892/ijmm.2020.4701
MLA
Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X., Zheng, L."Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway". International Journal of Molecular Medicine 46.4 (2020): 1347-1358.
Chicago
Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X., Zheng, L."Thymosin‑β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathway". International Journal of Molecular Medicine 46, no. 4 (2020): 1347-1358. https://doi.org/10.3892/ijmm.2020.4701
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