Silencing circRNA_001937 may inhibit cutaneous squamous cell carcinoma proliferation and induce apoptosis by preventing the sponging of the miRNA‑597‑3p/FOSL2 pathway

Gao,Ling; Jin,Hong‑Juan; Zhang,Duo; Lin,Quan

doi:10.3892/ijmm.2020.4723

Silencing circRNA_001937 may inhibit cutaneous squamous cell carcinoma proliferation and induce apoptosis by preventing the sponging of the miRNA‑597‑3p/FOSL2 pathway

Authors:
- Ling Gao
- Hong‑Juan Jin
- Duo Zhang
- Quan Lin
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Affiliations: Department of Radiation Oncology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Plastic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: September 10, 2020 https://doi.org/10.3892/ijmm.2020.4723
Pages: 1653-1660
Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) are reported to be aberrantly expressed and perform different functions in numerous types of tumor; however, their expression levels in cutaneous squamous cell carcinoma (CSCC) remain largely unclear. Thus, the purpose of the present study was to investigate the function of circRNA_001937 in CSCC. Differential circRNA expression profiles of CSCC were analyzed using the Arraystar Human circRNAs chip and reverse transcription‑quantitative PCR (RT‑qPCR); and the effects of circRNA_001937 on cell behavior, in particular its regulation over the microRNA (miRNA)‑597‑3p/Fos‑related antigen 2 (FOSL2) pathway, was investigated using a dual‑luciferase reporter assay, and verified using RT‑qPCR and western blotting. circRNA_001937 expression levels were significantly increased in CSCC tissues and cell lines compared with the corresponding adjacent tissues and control cells (P<0.05). The genetic silencing of circRNA_001937 with small interfering RNA significantly inhibited cell proliferation, and induced cell apoptosis (P<0.05). circRNA_001937 was observed to directly bind to miRNA‑597‑3p and serve as a sponge, which indirectly increased the expression levels of FOSL2, a miRNA‑597‑3p target gene. In conclusion, circRNA_001937 expression was increased in CSCC and silencing circRNA_001937 gene expression may inhibit CSCC progression by sponging the miRNA‑597‑3p/FOSL2 pathway.

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