Alteration of enzymes and their application to nucleic acid amplification (Review)
- Kiyoshi Yasukawa
- Itaru Yanagihara
- Shinsuke Fujiwara
Affiliations: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606‑8502, Japan, Department of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi, Osaka 594‑1101, Japan, Department of Bioscience, School of Science and Technology, Kwansei‑Gakuin University, Sanda, Hyogo 669‑1337, Japan
- Published online on: September 15, 2020 https://doi.org/10.3892/ijmm.2020.4726
Copyright: © Yasukawa
et al. This is an open access article distributed under the
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Since the discovery of polymerase chain reaction (PCR) in 1985, several methods have been developed to achieve nucleic acid amplification, and are currently used in various fields including clinical diagnosis and life science research. Thus, a wealth of information has accumulated regarding nucleic acid‑related enzymes. In this review, some nucleic acid‑related enzymes were selected and the recent advances in their modification along with their application to nucleic acid amplification were described. The discussion also focused on optimization of the corresponding reaction conditions. Using newly developed enzymes under well‑optimized reaction conditions, the sensitivity, specificity, and fidelity of nucleic acid tests can be improved successfully.