Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation

  • Authors:
    • Su Bin Cho
    • Won Sik Eum
    • Min Jea Shin
    • Hyeon Ji Yeo
    • Eun Ji Yeo
    • Yeon Joo Choi
    • Hyun Jung Kwon
    • Sung‑Woo Cho
    • Jinseu Park
    • Kyu Hyung Han
    • Keun Wook Lee
    • Jong Kook Park
    • Duk‑Soo Kim
    • Dae Won Kim
    • Soo Young Choi
  • View Affiliations

  • Published online on: December 8, 2020     https://doi.org/10.3892/ijmm.2020.4812
  • Pages: 751-760
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Abstract

Aldose reductase (AR) is known to detoxify aldehydes and prevent oxidative stress. Although AR exerts antioxidant effects, the role of AR in Parkinson's disease (PD) remains unclear. The objective of the present study was to investigate the protective effects of AR protein against 1‑methyl‑4‑phenylpyridinium (MPP+)‑induced SH‑SY5Y cell death and 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine (MPTP)‑induced PD in a mouse model using the cell permeable Tat‑AR fusion protein. The results revealed that when Tat‑AR protein was transduced into SH‑SY5Y cells, it markedly protected the cells against MPP+‑induced death and DNA fragmentation. It also reduced the activation of mitogen-activated protein kinase (MAPKs) and regulated the expression levels of Bcl‑2, Bax and caspase‑3. Immunohistochemical analysis revealed that when Tat‑AR protein was transduced into the substantia nigra (SN) of mice with PD, it markedly inhibited dopaminergic neuronal cell death. Therefore, Tat‑AR may be useful as a therapeutic protein for PD.
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February-2021
Volume 47 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cho SB, Eum WS, Shin MJ, Yeo HJ, Yeo EJ, Choi YJ, Kwon HJ, Cho SW, Park J, Han KH, Han KH, et al: Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation. Int J Mol Med 47: 751-760, 2021
APA
Cho, S.B., Eum, W.S., Shin, M.J., Yeo, H.J., Yeo, E.J., Choi, Y.J. ... Choi, S.Y. (2021). Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation. International Journal of Molecular Medicine, 47, 751-760. https://doi.org/10.3892/ijmm.2020.4812
MLA
Cho, S. B., Eum, W. S., Shin, M. J., Yeo, H. J., Yeo, E. J., Choi, Y. J., Kwon, H. J., Cho, S., Park, J., Han, K. H., Lee, K. W., Park, J. K., Kim, D., Kim, D. W., Choi, S. Y."Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation". International Journal of Molecular Medicine 47.2 (2021): 751-760.
Chicago
Cho, S. B., Eum, W. S., Shin, M. J., Yeo, H. J., Yeo, E. J., Choi, Y. J., Kwon, H. J., Cho, S., Park, J., Han, K. H., Lee, K. W., Park, J. K., Kim, D., Kim, D. W., Choi, S. Y."Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation". International Journal of Molecular Medicine 47, no. 2 (2021): 751-760. https://doi.org/10.3892/ijmm.2020.4812