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RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway

  • Authors:
    • Yu Guo
    • Huan Zhu
    • Xiangchen Li
    • Caiyun Ma
    • Yanan Li
    • Tingting Sun
    • Yuanyuan Wang
    • Chunjing Wang
    • Weijun Guan
    • Changqing Liu
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, School of Life Sciences, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Institute of Beijing Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 148
    |
    Published online on: June 11, 2021
       https://doi.org/10.3892/ijmm.2021.4981
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Abstract

Previous reports have demonstrated that RepSox can function as a replacement for cMyc and Sox2 in the reprogramming of cells into induced pluripotent stem cells (iPSCs), as well as increasing the levels of bone morphogenetic protein (BMP)‑3 and inducing the phosphorylation of Smad1 in mouse embryonic stem cells. In the present study, it was demonstrated that RepSox caused the visible morphological transformation of sheep fibroblasts; however, no significant alterations in cell proliferation, apoptosis or chromosome aberrations were observed. Moreover, RepSox increased the plasticity of long‑term cryopreserved sheep fibroblasts, and further promoted differentiation into adipocytes. RepSox treatment led to a notable decrease in the expression of components of the transforming growth factor (TGF)‑β signaling pathway, particularly Smad2/3 phosphorylation. RepSox also activated the BMP pathway, promoted the reprogramming of cells from fibroblasts into adipocytes and induced mesenchymal‑epithelial transition. It is worth noting that RepSox notably increased the expression of octamer‑binding transcription factor 4 and L‑Myc, whereas Sox2 and Nanog expression were not detected. The results of high‑throughput RNA sequencing revealed that the levels of differentially expressed genes (DEGs) involved in various metabolic processes were markedly upregulated in the RepSox‑treated fibroblasts, while the DEGs in the majority of signaling pathways were markedly downregulated. On the whole, the present study demonstrates that RepSox can promote the plasticity of sheep fibroblasts and facilitates the differentiation of adipocytes via increasing BMP expression and inhibiting the activation of the TGF‑β signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Zhu H, Li X, Ma C, Li Y, Sun T, Wang Y, Wang C, Guan W, Liu C, Liu C, et al: RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. Int J Mol Med 48: 148, 2021.
APA
Guo, Y., Zhu, H., Li, X., Ma, C., Li, Y., Sun, T. ... Liu, C. (2021). RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. International Journal of Molecular Medicine, 48, 148. https://doi.org/10.3892/ijmm.2021.4981
MLA
Guo, Y., Zhu, H., Li, X., Ma, C., Li, Y., Sun, T., Wang, Y., Wang, C., Guan, W., Liu, C."RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway". International Journal of Molecular Medicine 48.2 (2021): 148.
Chicago
Guo, Y., Zhu, H., Li, X., Ma, C., Li, Y., Sun, T., Wang, Y., Wang, C., Guan, W., Liu, C."RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway". International Journal of Molecular Medicine 48, no. 2 (2021): 148. https://doi.org/10.3892/ijmm.2021.4981
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Zhu H, Li X, Ma C, Li Y, Sun T, Wang Y, Wang C, Guan W, Liu C, Liu C, et al: RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. Int J Mol Med 48: 148, 2021.
APA
Guo, Y., Zhu, H., Li, X., Ma, C., Li, Y., Sun, T. ... Liu, C. (2021). RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. International Journal of Molecular Medicine, 48, 148. https://doi.org/10.3892/ijmm.2021.4981
MLA
Guo, Y., Zhu, H., Li, X., Ma, C., Li, Y., Sun, T., Wang, Y., Wang, C., Guan, W., Liu, C."RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway". International Journal of Molecular Medicine 48.2 (2021): 148.
Chicago
Guo, Y., Zhu, H., Li, X., Ma, C., Li, Y., Sun, T., Wang, Y., Wang, C., Guan, W., Liu, C."RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway". International Journal of Molecular Medicine 48, no. 2 (2021): 148. https://doi.org/10.3892/ijmm.2021.4981
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