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miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway

  • Authors:
    • Yan-Lu Liu
    • Wen-Hao Yang
    • Bao-Yi Chen
    • Juan Nie
    • Zi-Ren Su
    • Jing-Na Zheng
    • Shi-Ting Gong
    • Jian-Nan Chen
    • Dongbo Jiang
    • Yucui Li
  • View Affiliations / Copyright

    Affiliations: School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510006, P.R. China, Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 157
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    Published online on: June 25, 2021
       https://doi.org/10.3892/ijmm.2021.4990
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Abstract

MicroRNA (miR)‑29b is a key tumor regulator. It can inhibit tumor cell proliferation, induce apoptosis, suppress tumor invasion and migration, thus delaying tumor progression. Our previous studies revealed an increased level of miR‑29b in hepatoma 22 (H22) cells in ascites tumor‑bearing mice. The present study investigated the effect of miR‑29b on proliferation and apoptosis of hepatocellular carcinoma ascites H22 cells and its association with the transforming growth factor‑β1 (TGF‑β1) signaling pathway and p53‑mediated apoptotic pathway. Briefly, H22 cells were transfected with miR‑29b‑3p (hereinafter referred to as miR‑29b) mimic or miR‑29b inhibitor. MTS cell proliferation assay and flow cytometry were used to analyze cell viability and apoptosis. The expression change of the TGF‑β1 signaling pathway and p53‑mediated apoptotic pathway were detected by reverse transcription‑quantitative PCR, western blotting and immunofluorescence. Furthermore, cells were treated with exogenous TGF‑β1 and TGF‑β1 small interfering RNA to evaluate the crosstalk between TGF‑β1 and p53 under miR‑29b regulation. The overexpression of miR‑29b decreased cell viability, increased cell apoptosis, activated the TGF‑β1 signaling pathway and p53‑mediated apoptotic pathway. Conversely, these effects were reversed by the miR‑29b inhibitor. Moreover, the effect of miR‑29b mimic was further increased after treating cells with exogenous TGF‑β1. The activation of the TGF‑β1 signaling pathway and p53‑mediated apoptotic pathway induced by miR‑29b overexpression were reversed by TGF‑β1 inhibition. In summary, these data indicated that miR‑29b has an important role in proliferation and apoptosis of H22 cells by regulating the TGF‑β1 signaling pathway, the p53‑dependent apoptotic pathway, and the crosstalk between TGF‑β1 and p53.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Yang W, Chen B, Nie J, Su Z, Zheng J, Gong S, Chen J, Jiang D, Li Y, Li Y, et al: miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway. Int J Mol Med 48: 157, 2021.
APA
Liu, Y., Yang, W., Chen, B., Nie, J., Su, Z., Zheng, J. ... Li, Y. (2021). miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway. International Journal of Molecular Medicine, 48, 157. https://doi.org/10.3892/ijmm.2021.4990
MLA
Liu, Y., Yang, W., Chen, B., Nie, J., Su, Z., Zheng, J., Gong, S., Chen, J., Jiang, D., Li, Y."miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway". International Journal of Molecular Medicine 48.2 (2021): 157.
Chicago
Liu, Y., Yang, W., Chen, B., Nie, J., Su, Z., Zheng, J., Gong, S., Chen, J., Jiang, D., Li, Y."miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway". International Journal of Molecular Medicine 48, no. 2 (2021): 157. https://doi.org/10.3892/ijmm.2021.4990
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Yang W, Chen B, Nie J, Su Z, Zheng J, Gong S, Chen J, Jiang D, Li Y, Li Y, et al: miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway. Int J Mol Med 48: 157, 2021.
APA
Liu, Y., Yang, W., Chen, B., Nie, J., Su, Z., Zheng, J. ... Li, Y. (2021). miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway. International Journal of Molecular Medicine, 48, 157. https://doi.org/10.3892/ijmm.2021.4990
MLA
Liu, Y., Yang, W., Chen, B., Nie, J., Su, Z., Zheng, J., Gong, S., Chen, J., Jiang, D., Li, Y."miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway". International Journal of Molecular Medicine 48.2 (2021): 157.
Chicago
Liu, Y., Yang, W., Chen, B., Nie, J., Su, Z., Zheng, J., Gong, S., Chen, J., Jiang, D., Li, Y."miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway". International Journal of Molecular Medicine 48, no. 2 (2021): 157. https://doi.org/10.3892/ijmm.2021.4990
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