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Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling in vivo and in vitro

  • Authors:
    • Cong Cong
    • Xiaohong Yuan
    • Ying Hu
    • Wenjing Chen
    • Yong Wang
    • Lei Tao
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China, Department of Traumatic Orthopaedics, The Third Affiliated Hospital of Shandong First Medical University (Affiliated Hospital of Shandong Academy of Medical Sciences), Jinan, Shandong 250031, P.R. China, Department of Science and Technology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China, Department of Nephrology, The Third Affiliated Hospital of Shandong First Medical University (Affiliated Hospital of Shandong Academy of Medical Sciences), Jinan, Shandong 250031, P.R. China
    Copyright: © Cong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 161
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    Published online on: July 2, 2021
       https://doi.org/10.3892/ijmm.2021.4994
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Abstract

The present study investigated the function of sinigrin in angiotensin II (Ang II)‑induced renal damage. The results demonstrated that systolic blood pressure (SBP) and diastolic blood pressure (DBP) were increased in Ang II‑challenged rats, and sinigrin treatment inhibited their increase. The levels of blood urea nitrogen (BUN) and serum creatinine (SCR) were increased by Ang II in the rats, and these were reversed by sinigrin in a dose‑dependent manner. In addition, the Ang II‑induced elevation of urinary protein levels was inhibited by sinigrin treatment. Glomerular basement membrane thickness and ECM degradation markers, such as collagen I, collagen IV and fibronectin, were suppressed by sinigrin in the Ang II‑challenged rats. Moreover, the levels of inflammatory regulators, including tumor necrosis factor‑α (TNF‑α), interleukin‑6 (IL‑6) and monocyte chemoattractant protein‑1 (MCP‑1), were reduced following sinigrin treatment of the Ang II‑challenged rats and in Ang II‑exposed proximal tubule epithelial cells. Furthermore, the superoxide dismutase (SOD) and catalase (CAT) levels were downregulated, whereas the malondialdehyde (MDA) levels were upregulated by Ang II; these effects were reversed by sinigrin treatment in vivo and in vitro. Mechanistically, sinigrin inhibited the Ang II‑induced phosphorylation of ERK, p65 and IκBα. Thus, sinigrin attenuated Ang II‑induced renal injury by inactivating ERK and NF‑κB signaling. Sinigrin may thus prove to be a potential candidate for the treatment of hypertension‑induced kidney damage.
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Copy and paste a formatted citation
Spandidos Publications style
Cong C, Yuan X, Hu Y, Chen W, Wang Y and Tao L: Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>. Int J Mol Med 48: 161, 2021.
APA
Cong, C., Yuan, X., Hu, Y., Chen, W., Wang, Y., & Tao, L. (2021). Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>. International Journal of Molecular Medicine, 48, 161. https://doi.org/10.3892/ijmm.2021.4994
MLA
Cong, C., Yuan, X., Hu, Y., Chen, W., Wang, Y., Tao, L."Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>". International Journal of Molecular Medicine 48.2 (2021): 161.
Chicago
Cong, C., Yuan, X., Hu, Y., Chen, W., Wang, Y., Tao, L."Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>". International Journal of Molecular Medicine 48, no. 2 (2021): 161. https://doi.org/10.3892/ijmm.2021.4994
Copy and paste a formatted citation
x
Spandidos Publications style
Cong C, Yuan X, Hu Y, Chen W, Wang Y and Tao L: Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>. Int J Mol Med 48: 161, 2021.
APA
Cong, C., Yuan, X., Hu, Y., Chen, W., Wang, Y., & Tao, L. (2021). Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>. International Journal of Molecular Medicine, 48, 161. https://doi.org/10.3892/ijmm.2021.4994
MLA
Cong, C., Yuan, X., Hu, Y., Chen, W., Wang, Y., Tao, L."Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>". International Journal of Molecular Medicine 48.2 (2021): 161.
Chicago
Cong, C., Yuan, X., Hu, Y., Chen, W., Wang, Y., Tao, L."Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling <em>in vivo</em> and <em>in vitro</em>". International Journal of Molecular Medicine 48, no. 2 (2021): 161. https://doi.org/10.3892/ijmm.2021.4994
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