Open Access

Proteasome inhibitors decrease paclitaxel‑induced cell death in nasopharyngeal carcinoma with the accumulation of CDK1/cyclin B1

  • Authors:
    • Ling Hu
    • Xi Pan
    • Jinyue Hu
    • Hong Zeng
    • Xueting Liu
    • Manli Jiang
    • Binyuan Jiang
  • View Affiliations

  • Published online on: August 20, 2021     https://doi.org/10.3892/ijmm.2021.5026
  • Article Number: 193
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Southeast Asia is a region with high incidence of nasopharyngeal carcinoma (NPC). Paclitaxel is the mainstay for the treatment of advanced nasopharyngeal cancer. The present study investigated the effect of proteasome inhibitors on the therapeutic effect of paclitaxel and its related mechanism. The present data from Cell Counting Kit‑8 and flow cytometry assays demonstrated that appropriate concentrations of proteasome inhibitors (30 nM PS341 or 700 nM MG132) reduced the lethal effect of paclitaxel on the nasopharyngeal cancer cells. While 400 nM paclitaxel effectively inhibited cell division and induced cell death, proteasome inhibitors (PS341 30 nM or MG132 700 nM) could reverse these effects. Additionally, the western blotting results demonstrated accumulation of cell cycle regulation protein CDK1 and cyclin B1 in proteasome inhibitor‑treated cells. In addition, proteasome inhibitors combined with paclitaxel led to decreased MCL1 apoptosis regulator, BCL2 family member/Caspase‑9/poly (ADP‑ribose) polymerase apoptosis signaling triggered by CDK1/cyclin B1. Therefore, dysfunction of CDK1/cyclin B1 could be defining the loss of paclitaxel lethality against cancer cells, a phenomenon affirmed by the CDK1 inhibitor Ro3306. Overall, the present results demonstrated that a combination of paclitaxel with proteasome inhibitors or CDK1 inhibitors is antagonistic to effective clinical management of NPC.
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October-2021
Volume 48 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hu L, Pan X, Hu J, Zeng H, Liu X, Jiang M and Jiang B: Proteasome inhibitors decrease paclitaxel‑induced cell death in nasopharyngeal carcinoma with the accumulation of CDK1/cyclin B1. Int J Mol Med 48: 193, 2021
APA
Hu, L., Pan, X., Hu, J., Zeng, H., Liu, X., Jiang, M., & Jiang, B. (2021). Proteasome inhibitors decrease paclitaxel‑induced cell death in nasopharyngeal carcinoma with the accumulation of CDK1/cyclin B1. International Journal of Molecular Medicine, 48, 193. https://doi.org/10.3892/ijmm.2021.5026
MLA
Hu, L., Pan, X., Hu, J., Zeng, H., Liu, X., Jiang, M., Jiang, B."Proteasome inhibitors decrease paclitaxel‑induced cell death in nasopharyngeal carcinoma with the accumulation of CDK1/cyclin B1". International Journal of Molecular Medicine 48.4 (2021): 193.
Chicago
Hu, L., Pan, X., Hu, J., Zeng, H., Liu, X., Jiang, M., Jiang, B."Proteasome inhibitors decrease paclitaxel‑induced cell death in nasopharyngeal carcinoma with the accumulation of CDK1/cyclin B1". International Journal of Molecular Medicine 48, no. 4 (2021): 193. https://doi.org/10.3892/ijmm.2021.5026