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Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression

  • Authors:
    • Qihua Gu
    • Fangmin Chen
    • Ni Chen
    • Jing Wang
    • Zhao Li
    • Xinhao Deng
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Xiangya Hospital Affiliated to Central South University, Changsha, Hunan 410008, P.R. China, Department of Pathology, Xiangya Hospital Affiliated to Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 220
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    Published online on: October 20, 2021
       https://doi.org/10.3892/ijmm.2021.5053
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Abstract

Epigallocatechin‑3‑gallate (EGCG) has been demonstrated to exhibit anticancer effects; however, the mechanisms behind these are not yet clear. The objective of the present study was to assess the effect of EGCG on smoking‑induced, precancerous, bronchial epithelial cell lesions and determine a potential protective mechanism. Human bronchial epithelial (HBE) cells were treated with cigarette smoke extract (CSE). Benzopyrene‑DNA adducts were detected by immunofluorescence cytochemistry. Changes to microRNA (miRNA) expression levels were detected via microarray. The effects of EGCG on smoke‑induced benzopyrene‑DNA adduct formation and the subsequent change in miRNA expression were analyzed. Subsequently, the protective effect of EGCG on smoke inhalation‑induced precancerous lesions was investigated. The expression levels of miRNA target genes were also analyzed. After CSE treatment, benzopyrene‑DNA adducts appeared in HBE cells, along with a resultant change in miRNA expression. EGCG inhibited the effects of CSE exposure; benzopyrene‑DNA adduct formation was reduced and miRNA expression changes were suppressed. In vivo, EGCG significantly reduced benzopyrene‑DNA adduct formation and the subsequent development of precancerous lesions in rat lungs induced by cigarette smoke inhalation. Moreover, EGCG downregulated CYP1A1 overexpression, a target gene of multiple smoking‑induced miRNAs, in rat lungs. EGCG may reduce the risk of lung cancer by downregulating the expression of the key gene CYP1A1, preventing the formation of smoking‑induced benzopyrene‑DNA adducts and alleviating smoking‑induced bronchial epithelial dysplasia and heterogeneity.
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Spandidos Publications style
Gu Q, Chen F, Chen N, Wang J, Li Z and Deng X: Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression. Int J Mol Med 48: 220, 2021.
APA
Gu, Q., Chen, F., Chen, N., Wang, J., Li, Z., & Deng, X. (2021). Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression. International Journal of Molecular Medicine, 48, 220. https://doi.org/10.3892/ijmm.2021.5053
MLA
Gu, Q., Chen, F., Chen, N., Wang, J., Li, Z., Deng, X."Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression". International Journal of Molecular Medicine 48.6 (2021): 220.
Chicago
Gu, Q., Chen, F., Chen, N., Wang, J., Li, Z., Deng, X."Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression". International Journal of Molecular Medicine 48, no. 6 (2021): 220. https://doi.org/10.3892/ijmm.2021.5053
Copy and paste a formatted citation
x
Spandidos Publications style
Gu Q, Chen F, Chen N, Wang J, Li Z and Deng X: Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression. Int J Mol Med 48: 220, 2021.
APA
Gu, Q., Chen, F., Chen, N., Wang, J., Li, Z., & Deng, X. (2021). Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression. International Journal of Molecular Medicine, 48, 220. https://doi.org/10.3892/ijmm.2021.5053
MLA
Gu, Q., Chen, F., Chen, N., Wang, J., Li, Z., Deng, X."Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression". International Journal of Molecular Medicine 48.6 (2021): 220.
Chicago
Gu, Q., Chen, F., Chen, N., Wang, J., Li, Z., Deng, X."Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression". International Journal of Molecular Medicine 48, no. 6 (2021): 220. https://doi.org/10.3892/ijmm.2021.5053
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