Extracellular vesicles from tonsil‑derived mesenchymal stromal cells show anti‑tumor effect via miR‑199a‑3p

Choi,Da-Won; Cho,Kyung-Ah; Kim,Jungwoo; Lee,Hyun-Ji; Kim,Yu-Hee; Park,Jang-Won; Woo,So-Youn

doi:10.3892/ijmm.2021.5054

Extracellular vesicles from tonsil‑derived mesenchymal stromal cells show anti‑tumor effect via miR‑199a‑3p

Authors:
- Da-Won Choi
- Kyung-Ah Cho
- Jungwoo Kim
- Hyun-Ji Lee
- Yu-Hee Kim
- Jang-Won Park
- So-Youn Woo
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Affiliations: Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 07804, Republic of Korea, Department of Orthopaedic Surgery, College of Medicine, Ewha Womans University, Seoul 07804, Republic of Korea
Published online on: October 21, 2021 https://doi.org/10.3892/ijmm.2021.5054
Article Number: 221
Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mesenchymal stem cells (MSCs) are mesoderm‑originated adult SCs that possess multidirectional differentiation potential. MSCs migrate to injured tissue and secrete a range of paracrine factors that induce regeneration in damaged tissue and exert immune modulation. Because tumor progression is dependent on cross‑talk between the tumor and its microenvironment, MSCs also produce extracellular vesicles (EVs) that mediate information transfer in the tumor microenvironment. However, the effect of MSC‑derived EVs on tumor development and progression is still controversial. To date, tonsil‑derived MSCs (T‑MSCs) have been shown to possess all the defined characteristics of MSCs and show distinctive features of differential potential and immune modulation. To observe the effect of soluble mediators from T‑MSCs on tumor growth, human liver cancer cell line (HepG2) cells were injected into nude mice and HepG2 cell scratch migration assay was performed using conditioned medium (CM) of T‑MSCs. T‑MSC CM inhibited tumor growth and progression and it was hypothesized that EVs from T‑MSCs could inhibit tumor progression. microRNA (miRNA or miR) sequencing using five different origins of T‑MSC‑derived EVs was performed and highly expressed miRNAs, such as miR‑199a‑3p, miR‑214‑3p, miR‑199a‑5p and miR‑199b‑5p, were selected. T‑MSCs inhibited tumor growth and HepG2 cell migration, potentially via miR‑199a‑3p targeting CD151, integrin α3 and 6 in HepG2 cells.

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