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Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways

  • Authors:
    • Yaqing Zhang
    • Yonghua Li
    • Qilong Wang
    • Dongyu Zheng
    • Xue Feng
    • Wei Zhao
    • Linlin Cai
    • Qingqing Zhang
    • Haitao Xu
    • Hailong Fu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Changzheng Hospital, Naval Medical University, Shanghai 200003, P.R. China, Department of Liver Surgery and Liver Transplantation Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Department of Obstetrics and Gynecology, Inner Mongolia Autonomous Region Corps Hospital of Chinese People's Armed Police Force, Hohhot, Inner Mongolia Autonomous Region 010040, P.R. China, Department of Anesthesiology, Wuxi People's Hospital, Wuxi, Jiangsu 214023, P.R. China, Department of Anesthesiology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 13
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    Published online on: December 3, 2021
       https://doi.org/10.3892/ijmm.2021.5068
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Abstract

Exosomes are an emerging therapeutic tool for the treatment of tissue injuries. In the present study, the protective effect of isolated exosomes from adipose‑derived stem cells (ADSCs‑exo) against hepatic ischemia‑reperfusion (I/R) injury was explored. Hepatic I/R injury was achieved by inducing ischemia for 60 min followed by reperfusion for 2 and 6 h. Pre‑treatment with ADSCs‑exo revealed a significant reduction in necrosis and apoptosis in liver tissue induced by I/R injury. Hypoxic oxidative stress was managed by exosome‑mediated reduced reactive oxygen species and increased superoxide dismutase that in turn protected mitochondrial damage and apoptosis. Reduction in inflammatory mediators such as IL‑1β and TNF‑α was also observed and protection of hepatocytes from I/R injury was evidenced by a significant decrease in biochemical markers of liver damage (alanine transaminase, aspartate transaminase and lactate dehydrogenase). Exosomal prostaglandin E2 (PGE2)‑mediated ERK1/2 and GSK‑3β phosphorylation were revealed to increase Bcl‑2 and decrease Bax expression with mitochondrial permeability transition pore‑inhibition which may be considered a prime mechanism of exosome‑mediated hepatoprotection. In conclusion, our results indicated that ADSCs‑exo pre‑treatment is effective in protecting liver I/R injury.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Li Y, Wang Q, Zheng D, Feng X, Zhao W, Cai L, Zhang Q, Xu H, Fu H, Fu H, et al: Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways. Int J Mol Med 49: 13, 2022.
APA
Zhang, Y., Li, Y., Wang, Q., Zheng, D., Feng, X., Zhao, W. ... Fu, H. (2022). Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways. International Journal of Molecular Medicine, 49, 13. https://doi.org/10.3892/ijmm.2021.5068
MLA
Zhang, Y., Li, Y., Wang, Q., Zheng, D., Feng, X., Zhao, W., Cai, L., Zhang, Q., Xu, H., Fu, H."Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways". International Journal of Molecular Medicine 49.2 (2022): 13.
Chicago
Zhang, Y., Li, Y., Wang, Q., Zheng, D., Feng, X., Zhao, W., Cai, L., Zhang, Q., Xu, H., Fu, H."Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways". International Journal of Molecular Medicine 49, no. 2 (2022): 13. https://doi.org/10.3892/ijmm.2021.5068
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x
Spandidos Publications style
Zhang Y, Li Y, Wang Q, Zheng D, Feng X, Zhao W, Cai L, Zhang Q, Xu H, Fu H, Fu H, et al: Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways. Int J Mol Med 49: 13, 2022.
APA
Zhang, Y., Li, Y., Wang, Q., Zheng, D., Feng, X., Zhao, W. ... Fu, H. (2022). Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways. International Journal of Molecular Medicine, 49, 13. https://doi.org/10.3892/ijmm.2021.5068
MLA
Zhang, Y., Li, Y., Wang, Q., Zheng, D., Feng, X., Zhao, W., Cai, L., Zhang, Q., Xu, H., Fu, H."Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways". International Journal of Molecular Medicine 49.2 (2022): 13.
Chicago
Zhang, Y., Li, Y., Wang, Q., Zheng, D., Feng, X., Zhao, W., Cai, L., Zhang, Q., Xu, H., Fu, H."Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways". International Journal of Molecular Medicine 49, no. 2 (2022): 13. https://doi.org/10.3892/ijmm.2021.5068
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